2.50
Hdl Handle:
http://hdl.handle.net/10033/36032
Title:
Isolation of alkali-tolerant benzene-degrading bacteria from a contaminated aquifer.
Authors:
Fahy, A; Ball, A S; Lethbridge, G; Timmis, K N; McGenity, T J
Abstract:
AIMS: To isolate benzene-degrading strains from neutral and alkaline groundwaters contaminated by benzene, toluene, ethylbenzene, xylenes (BTEX) from the SIReN aquifer, UK, and to test their effective pH range and ability to degrade TEX. METHODS AND RESULTS: The 14 isolates studied had an optimum pH for growth of 8, and could degrade benzene to below detection level (1 microg l(-1)). Five Rhodococcus erythropolis strains were able to metabolize benzene up to pH 9, two distinct R. erythropolis strains to pH 10, and one Arthrobacter strain to pH 8.5. These Actinobacteria also degraded benzene at least down to pH 5.5. Six other isolates, a Hydrogenophaga and five Pseudomonas strains, had a narrower pH tolerance for benzene degradation (pH 6 to 8.5), and could metabolize toluene; in addition, the Hydrogenophaga and two Pseudomonas strains utilized o-, m- or p-xylenes. None of these strains degraded ethylbenzene. CONCLUSIONS: Phylogenetically distinct isolates, able to degrade BTX compounds, were obtained, and some degraded benzene at high pH. SIGNIFICANCE AND IMPACT OF THE STUDY: High pH has previously been found to inhibit in situ degradation of benzene, a widespread, carcinogenic groundwater contaminant. These benzene-degrading organisms therefore have potential applications in the remediation or natural attenuation of alkaline waters.
Affiliation:
Department of Biological Sciences, University of Essex, Wivenhoe Park, Colchester, UK. afahy@essex.ac.uk
Citation:
Isolation of alkali-tolerant benzene-degrading bacteria from a contaminated aquifer. 2008, 47 (1):60-6 Lett. Appl. Microbiol.
Journal:
Letters in applied microbiology
Issue Date:
Jul-2008
URI:
http://hdl.handle.net/10033/36032
DOI:
10.1111/j.1472-765X.2008.02386.x
PubMed ID:
18544140
Type:
Article
Language:
en
ISSN:
1472-765X
Appears in Collections:
Publications of RG Environmental Microbiology (UMW)

Full metadata record

DC FieldValue Language
dc.contributor.authorFahy, A-
dc.contributor.authorBall, A S-
dc.contributor.authorLethbridge, G-
dc.contributor.authorTimmis, K N-
dc.contributor.authorMcGenity, T J-
dc.date.accessioned2008-08-21T08:37:58Z-
dc.date.available2008-08-21T08:37:58Z-
dc.date.issued2008-07-
dc.identifier.citationIsolation of alkali-tolerant benzene-degrading bacteria from a contaminated aquifer. 2008, 47 (1):60-6 Lett. Appl. Microbiol.en
dc.identifier.issn1472-765X-
dc.identifier.pmid18544140-
dc.identifier.doi10.1111/j.1472-765X.2008.02386.x-
dc.identifier.urihttp://hdl.handle.net/10033/36032-
dc.description.abstractAIMS: To isolate benzene-degrading strains from neutral and alkaline groundwaters contaminated by benzene, toluene, ethylbenzene, xylenes (BTEX) from the SIReN aquifer, UK, and to test their effective pH range and ability to degrade TEX. METHODS AND RESULTS: The 14 isolates studied had an optimum pH for growth of 8, and could degrade benzene to below detection level (1 microg l(-1)). Five Rhodococcus erythropolis strains were able to metabolize benzene up to pH 9, two distinct R. erythropolis strains to pH 10, and one Arthrobacter strain to pH 8.5. These Actinobacteria also degraded benzene at least down to pH 5.5. Six other isolates, a Hydrogenophaga and five Pseudomonas strains, had a narrower pH tolerance for benzene degradation (pH 6 to 8.5), and could metabolize toluene; in addition, the Hydrogenophaga and two Pseudomonas strains utilized o-, m- or p-xylenes. None of these strains degraded ethylbenzene. CONCLUSIONS: Phylogenetically distinct isolates, able to degrade BTX compounds, were obtained, and some degraded benzene at high pH. SIGNIFICANCE AND IMPACT OF THE STUDY: High pH has previously been found to inhibit in situ degradation of benzene, a widespread, carcinogenic groundwater contaminant. These benzene-degrading organisms therefore have potential applications in the remediation or natural attenuation of alkaline waters.en
dc.language.isoenen
dc.subject.meshAlkaliesen
dc.subject.meshBacteriaen
dc.subject.meshBacteria, Aerobicen
dc.subject.meshBenzeneen
dc.subject.meshBiodegradation, Environmentalen
dc.subject.meshWater Microbiologyen
dc.subject.meshXylenesen
dc.titleIsolation of alkali-tolerant benzene-degrading bacteria from a contaminated aquifer.en
dc.typeArticleen
dc.contributor.departmentDepartment of Biological Sciences, University of Essex, Wivenhoe Park, Colchester, UK. afahy@essex.ac.uken
dc.identifier.journalLetters in applied microbiologyen
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