Protein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overview.

2.50
Hdl Handle:
http://hdl.handle.net/10033/47714
Title:
Protein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overview.
Authors:
Gasser, Brigitte; Saloheimo, Markku; Rinas, Ursula; Dragosits, Martin; Rodríguez-Carmona, Escarlata; Baumann, Kristin; Giuliani, Maria; Parrilli, Ermenegilda; Branduardi, Paola; Lang, Christine; Porro, Danilo; Ferrer, Pau; Tutino, Maria Luisa; Mattanovich, Diethard; Villaverde, Antonio
Abstract:
ABSTRACT: Different species of microorganisms including yeasts, filamentous fungi and bacteria have been used in the past 25 years for the controlled production of foreign proteins of scientific, pharmacological or industrial interest. A major obstacle for protein production processes and a limit to overall success has been the abundance of misfolded polypeptides, which fail to reach their native conformation. The presence of misfolded or folding-reluctant protein species causes considerable stress in host cells. The characterization of such adverse conditions and the elicited cell responses have permitted to better understand the physiology and molecular biology of conformational stress. Therefore, microbial cell factories for recombinant protein production are depicted here as a source of knowledge that has considerably helped to picture the extremely rich landscape of in vivo protein folding, and the main cellular players of this complex process are described for the most important cell factories used for biotechnological purposes.
Affiliation:
University of Natural Resources and Applied Life Sciences Vienna, Department of Biotechnology, Vienna, Austria. diethard.mattanovich@boku.ac.at.
Citation:
Protein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overview. 2008, 7:11 Microb. Cell Fact.
Journal:
Microbial cell factories
Issue Date:
2008
URI:
http://hdl.handle.net/10033/47714
DOI:
10.1186/1475-2859-7-11
PubMed ID:
18394160
Additional Links:
http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=18394160
Type:
Article
Language:
en
ISSN:
1475-2859
Appears in Collections:
Publications from Division of Molekulare Struktur Biologie (MOSB)

Full metadata record

DC FieldValue Language
dc.contributor.authorGasser, Brigitte-
dc.contributor.authorSaloheimo, Markku-
dc.contributor.authorRinas, Ursula-
dc.contributor.authorDragosits, Martin-
dc.contributor.authorRodríguez-Carmona, Escarlata-
dc.contributor.authorBaumann, Kristin-
dc.contributor.authorGiuliani, Maria-
dc.contributor.authorParrilli, Ermenegilda-
dc.contributor.authorBranduardi, Paola-
dc.contributor.authorLang, Christine-
dc.contributor.authorPorro, Danilo-
dc.contributor.authorFerrer, Pau-
dc.contributor.authorTutino, Maria Luisa-
dc.contributor.authorMattanovich, Diethard-
dc.contributor.authorVillaverde, Antonio-
dc.date.accessioned2009-01-19T15:22:12Z-
dc.date.available2009-01-19T15:22:12Z-
dc.date.issued2008-
dc.identifier.citationProtein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overview. 2008, 7:11 Microb. Cell Fact.en
dc.identifier.issn1475-2859-
dc.identifier.pmid18394160-
dc.identifier.doi10.1186/1475-2859-7-11-
dc.identifier.urihttp://hdl.handle.net/10033/47714-
dc.description.abstractABSTRACT: Different species of microorganisms including yeasts, filamentous fungi and bacteria have been used in the past 25 years for the controlled production of foreign proteins of scientific, pharmacological or industrial interest. A major obstacle for protein production processes and a limit to overall success has been the abundance of misfolded polypeptides, which fail to reach their native conformation. The presence of misfolded or folding-reluctant protein species causes considerable stress in host cells. The characterization of such adverse conditions and the elicited cell responses have permitted to better understand the physiology and molecular biology of conformational stress. Therefore, microbial cell factories for recombinant protein production are depicted here as a source of knowledge that has considerably helped to picture the extremely rich landscape of in vivo protein folding, and the main cellular players of this complex process are described for the most important cell factories used for biotechnological purposes.en
dc.language.isoenen
dc.relation.urlhttp://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=18394160en
dc.titleProtein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overview.en
dc.typeArticleen
dc.contributor.departmentUniversity of Natural Resources and Applied Life Sciences Vienna, Department of Biotechnology, Vienna, Austria. diethard.mattanovich@boku.ac.at.en
dc.identifier.journalMicrobial cell factoriesen

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