2.50
Hdl Handle:
http://hdl.handle.net/10033/47810
Title:
Marine biofilm bacteria evade eukaryotic predation by targeted chemical defense.
Authors:
Matz, Carsten; Webb, Jeremy S; Schupp, Peter J; Phang, Shui Yen; Penesyan, Anahit; Egan, Suhelen; Steinberg, Peter; Kjelleberg, Staffan
Abstract:
Many plants and animals are defended from predation or herbivory by inhibitory secondary metabolites, which in the marine environment are very common among sessile organisms. Among bacteria, where there is the greatest metabolic potential, little is known about chemical defenses against bacterivorous consumers. An emerging hypothesis is that sessile bacterial communities organized as biofilms serve as bacterial refuge from predation. By testing growth and survival of two common bacterivorous nanoflagellates, we find evidence that chemically mediated resistance against protozoan predators is common among biofilm populations in a diverse set of marine bacteria. Using bioassay-guided chemical and genetic analysis, we identified one of the most effective antiprotozoal compounds as violacein, an alkaloid that we demonstrate is produced predominately within biofilm cells. Nanomolar concentrations of violacein inhibit protozoan feeding by inducing a conserved eukaryotic cell death program. Such biofilm-specific chemical defenses could contribute to the successful persistence of biofilm bacteria in various environments and provide the ecological and evolutionary context for a number of eukaryote-targeting bacterial metabolites.
Affiliation:
School of Biotechnology and Biomolecular Sciences and Centre for Marine Bio-Innovation, University of New South Wales, Sydney, Australia.
Citation:
Marine biofilm bacteria evade eukaryotic predation by targeted chemical defense. 2008, 3 (7):e2744 PLoS ONE
Journal:
PLoS ONE
Issue Date:
2008
URI:
http://hdl.handle.net/10033/47810
DOI:
10.1371/journal.pone.0002744
PubMed ID:
18648491
Additional Links:
http://www.plosone.org/article/info:doi/10.1371/journal.pone.0002744
Type:
Article
Language:
en
ISSN:
1932-6203
Appears in Collections:
Publications of Dept. Cell Biology (ZB)

Full metadata record

DC FieldValue Language
dc.contributor.authorMatz, Carsten-
dc.contributor.authorWebb, Jeremy S-
dc.contributor.authorSchupp, Peter J-
dc.contributor.authorPhang, Shui Yen-
dc.contributor.authorPenesyan, Anahit-
dc.contributor.authorEgan, Suhelen-
dc.contributor.authorSteinberg, Peter-
dc.contributor.authorKjelleberg, Staffan-
dc.date.accessioned2009-01-21T15:32:37Z-
dc.date.available2009-01-21T15:32:37Z-
dc.date.issued2008-
dc.identifier.citationMarine biofilm bacteria evade eukaryotic predation by targeted chemical defense. 2008, 3 (7):e2744 PLoS ONEen
dc.identifier.issn1932-6203-
dc.identifier.pmid18648491-
dc.identifier.doi10.1371/journal.pone.0002744-
dc.identifier.urihttp://hdl.handle.net/10033/47810-
dc.description.abstractMany plants and animals are defended from predation or herbivory by inhibitory secondary metabolites, which in the marine environment are very common among sessile organisms. Among bacteria, where there is the greatest metabolic potential, little is known about chemical defenses against bacterivorous consumers. An emerging hypothesis is that sessile bacterial communities organized as biofilms serve as bacterial refuge from predation. By testing growth and survival of two common bacterivorous nanoflagellates, we find evidence that chemically mediated resistance against protozoan predators is common among biofilm populations in a diverse set of marine bacteria. Using bioassay-guided chemical and genetic analysis, we identified one of the most effective antiprotozoal compounds as violacein, an alkaloid that we demonstrate is produced predominately within biofilm cells. Nanomolar concentrations of violacein inhibit protozoan feeding by inducing a conserved eukaryotic cell death program. Such biofilm-specific chemical defenses could contribute to the successful persistence of biofilm bacteria in various environments and provide the ecological and evolutionary context for a number of eukaryote-targeting bacterial metabolites.en
dc.language.isoenen
dc.relation.urlhttp://www.plosone.org/article/info:doi/10.1371/journal.pone.0002744en
dc.subject.meshAlgae, Greenen
dc.subject.meshAntiprotozoal Agentsen
dc.subject.meshApoptosisen
dc.subject.meshBacteriaen
dc.subject.meshBacterial Physiological Phenomenaen
dc.subject.meshBiofilmsen
dc.subject.meshBiological Assayen
dc.subject.meshCatalysisen
dc.subject.meshEcologyen
dc.subject.meshEcosystemen
dc.subject.meshKineticsen
dc.subject.meshModels, Geneticen
dc.subject.meshPlanktonen
dc.subject.meshWater Microbiologyen
dc.titleMarine biofilm bacteria evade eukaryotic predation by targeted chemical defense.en
dc.typeArticleen
dc.contributor.departmentSchool of Biotechnology and Biomolecular Sciences and Centre for Marine Bio-Innovation, University of New South Wales, Sydney, Australia.en
dc.identifier.journalPLoS ONEen

Related articles on PubMed

This item is licensed under a Creative Commons License
Creative Commons
All Items in HZI are protected by copyright, with all rights reserved, unless otherwise indicated.