Differentially oriented populations of actin filaments generated in lamellipodia collaborate in pushing and pausing at the cell front.

2.50
Hdl Handle:
http://hdl.handle.net/10033/48244
Title:
Differentially oriented populations of actin filaments generated in lamellipodia collaborate in pushing and pausing at the cell front.
Authors:
Koestler, Stefan A; Auinger, Sonja; Vinzenz, Marlene; Rottner, Klemens; Small, J Victor
Abstract:
Eukaryotic cells advance in phases of protrusion, pause and withdrawal. Protrusion occurs in lamellipodia, which are composed of diagonal networks of actin filaments, and withdrawal terminates with the formation of actin bundles parallel to the cell edge. Using correlated live-cell imaging and electron microscopy, we have shown that actin filaments in protruding lamellipodia subtend angles from 15-90 degrees to the front, and that transitions from protrusion to pause are associated with a proportional increase in filaments oriented more parallel to the cell edge. Microspike bundles of actin filaments also showed a wide angular distribution and correspondingly variable bilateral polymerization rates along the cell front. We propose that the angular shift of filaments in lamellipodia serves in adapting to slower protrusion rates while maintaining the filament densities required for structural support; further, we suggest that single filaments and microspike bundles contribute to the construction of the lamella behind and to the formation of the cell edge when protrusion ceases. Our findings provide an explanation for the variable turnover dynamics of actin filaments in lamellipodia observed by fluorescence speckle microscopy and are inconsistent with a current model of lamellipodia structure that features actin filaments branching at 70 degrees in a dendritic array.
Affiliation:
Institute of Molecular Biotechnology, Austrian Academy of Sciences, Dr. Bohr-Gasse 3, 1030, Vienna, Austria.
Citation:
Differentially oriented populations of actin filaments generated in lamellipodia collaborate in pushing and pausing at the cell front. 2008, 10 (3):306-13 Nat. Cell Biol.
Journal:
Nature cell biology
Issue Date:
Mar-2008
URI:
http://hdl.handle.net/10033/48244
DOI:
10.1038/ncb1692
PubMed ID:
18278037
Type:
Article
Language:
en
ISSN:
1476-4679
Appears in Collections:
Publications of RG Cytoskeleton Dynamics (CYD)

Full metadata record

DC FieldValue Language
dc.contributor.authorKoestler, Stefan A-
dc.contributor.authorAuinger, Sonja-
dc.contributor.authorVinzenz, Marlene-
dc.contributor.authorRottner, Klemens-
dc.contributor.authorSmall, J Victor-
dc.date.accessioned2009-01-30T14:11:51Z-
dc.date.available2009-01-30T14:11:51Z-
dc.date.issued2008-03-
dc.identifier.citationDifferentially oriented populations of actin filaments generated in lamellipodia collaborate in pushing and pausing at the cell front. 2008, 10 (3):306-13 Nat. Cell Biol.en
dc.identifier.issn1476-4679-
dc.identifier.pmid18278037-
dc.identifier.doi10.1038/ncb1692-
dc.identifier.urihttp://hdl.handle.net/10033/48244-
dc.description.abstractEukaryotic cells advance in phases of protrusion, pause and withdrawal. Protrusion occurs in lamellipodia, which are composed of diagonal networks of actin filaments, and withdrawal terminates with the formation of actin bundles parallel to the cell edge. Using correlated live-cell imaging and electron microscopy, we have shown that actin filaments in protruding lamellipodia subtend angles from 15-90 degrees to the front, and that transitions from protrusion to pause are associated with a proportional increase in filaments oriented more parallel to the cell edge. Microspike bundles of actin filaments also showed a wide angular distribution and correspondingly variable bilateral polymerization rates along the cell front. We propose that the angular shift of filaments in lamellipodia serves in adapting to slower protrusion rates while maintaining the filament densities required for structural support; further, we suggest that single filaments and microspike bundles contribute to the construction of the lamella behind and to the formation of the cell edge when protrusion ceases. Our findings provide an explanation for the variable turnover dynamics of actin filaments in lamellipodia observed by fluorescence speckle microscopy and are inconsistent with a current model of lamellipodia structure that features actin filaments branching at 70 degrees in a dendritic array.en
dc.language.isoenen
dc.subject.meshActinsen
dc.subject.meshAnimalsen
dc.subject.meshCell Movementen
dc.subject.meshCell Polarityen
dc.subject.meshDendritesen
dc.subject.meshGene Expression Regulationen
dc.subject.meshMelanoma, Experimentalen
dc.subject.meshMiceen
dc.subject.meshMicrofilamentsen
dc.subject.meshMicroscopy, Electronen
dc.subject.meshMicroscopy, Fluorescenceen
dc.subject.meshModels, Biologicalen
dc.subject.meshPseudopodiaen
dc.subject.meshTransfectionen
dc.titleDifferentially oriented populations of actin filaments generated in lamellipodia collaborate in pushing and pausing at the cell front.en
dc.typeArticleen
dc.contributor.departmentInstitute of Molecular Biotechnology, Austrian Academy of Sciences, Dr. Bohr-Gasse 3, 1030, Vienna, Austria.en
dc.identifier.journalNature cell biologyen

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