2.50
Hdl Handle:
http://hdl.handle.net/10033/528130
Title:
Towards the generation of B-cell receptor retrogenic mice.
Authors:
Freitag, Jenny; Heink, Sylvia; Roth, Edith; Wittmann, Jürgen; Jäck, Hans-Martin; Kamradt, Thomas
Abstract:
Transgenic expression of B- and T-cell receptors (BCRs and TCRs, respectively) has been a standard tool to study lymphocyte development and function in vivo. The generation of transgenic mice is time-consuming and, therefore, a faster method to study the biology of defined lymphocyte receptors in vivo would be highly welcome. Using 2A peptide-linked multicistronic retroviral vectors to transduce stem cells, TCRs can be expressed rapidly in mice of any background. We aimed at adopting this retrogenic technology to the in vivo expression of BCRs. Using a well characterised BCR specific for hen egg lysozyme (HEL), we achieved surface expression of the retrogenically encoded BCR in a Rag-deficient pro B-cell line in vitro. In vivo, retrogenic BCRs were detectable only intracellularly but not on the surface of B cells from wild type or Rag2-deficient mice. This data, together with the fact that no BCR retrogenic mouse model has been published in the 7 years since the method was originally published for TCRs, strongly suggests that achieving BCR-expression in vivo with retrogenic technology is highly challenging if not impossible.
Affiliation:
Institute for Infection Immunology, Centre for Experimental and Clinical Infection Research, a joint venture between the Medical, School Hannover (MHH) and the Helmholtz Centre for Infection Research (HZI), Hannover, Germany.
Citation:
Towards the generation of B-cell receptor retrogenic mice. 2014, 9 (10):e109199 PLoS ONE
Journal:
PloS one
Issue Date:
2014
URI:
http://hdl.handle.net/10033/528130
DOI:
10.1371/journal.pone.0109199
PubMed ID:
25296340
Type:
Article
Language:
en
ISSN:
1932-6203
Appears in Collections:
publications of the TwinCore unit Infection immunology

Full metadata record

DC FieldValue Language
dc.contributor.authorFreitag, Jennyen
dc.contributor.authorHeink, Sylviaen
dc.contributor.authorRoth, Edithen
dc.contributor.authorWittmann, Jürgenen
dc.contributor.authorJäck, Hans-Martinen
dc.contributor.authorKamradt, Thomasen
dc.date.accessioned2015-04-10T12:52:31Zen
dc.date.available2015-04-10T12:52:31Zen
dc.date.issued2014en
dc.identifier.citationTowards the generation of B-cell receptor retrogenic mice. 2014, 9 (10):e109199 PLoS ONEen
dc.identifier.issn1932-6203en
dc.identifier.pmid25296340en
dc.identifier.doi10.1371/journal.pone.0109199en
dc.identifier.urihttp://hdl.handle.net/10033/528130en
dc.description.abstractTransgenic expression of B- and T-cell receptors (BCRs and TCRs, respectively) has been a standard tool to study lymphocyte development and function in vivo. The generation of transgenic mice is time-consuming and, therefore, a faster method to study the biology of defined lymphocyte receptors in vivo would be highly welcome. Using 2A peptide-linked multicistronic retroviral vectors to transduce stem cells, TCRs can be expressed rapidly in mice of any background. We aimed at adopting this retrogenic technology to the in vivo expression of BCRs. Using a well characterised BCR specific for hen egg lysozyme (HEL), we achieved surface expression of the retrogenically encoded BCR in a Rag-deficient pro B-cell line in vitro. In vivo, retrogenic BCRs were detectable only intracellularly but not on the surface of B cells from wild type or Rag2-deficient mice. This data, together with the fact that no BCR retrogenic mouse model has been published in the 7 years since the method was originally published for TCRs, strongly suggests that achieving BCR-expression in vivo with retrogenic technology is highly challenging if not impossible.en
dc.language.isoenen
dc.titleTowards the generation of B-cell receptor retrogenic mice.en
dc.typeArticleen
dc.contributor.departmentInstitute for Infection Immunology, Centre for Experimental and Clinical Infection Research, a joint venture between the Medical, School Hannover (MHH) and the Helmholtz Centre for Infection Research (HZI), Hannover, Germany.en
dc.identifier.journalPloS oneen
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