2.50
Hdl Handle:
http://hdl.handle.net/10033/552680
Title:
Stable mammalian producer cell lines for structural biology.
Authors:
Büssow, Konrad ( 0000-0003-2031-5942 )
Abstract:
The mammalian cell lines HEK293 and CHO have become important expression hosts in structural biology. Generating stable mammalian cell lines remains essential for studying the function and structure of recombinant proteins, despite the emergence of highly efficient transient transfection protocols. Production with stable cell lines can be scaled up easily and high volumetric product yield can be achieved. Protein structure reports of the past two years that used stable cell lines were surveyed for this review. Well-established techniques and novel approaches for generating stable cell lines and stable cell pools are presented, including cell sorting, site-specific recombination, transposons, the Lentivirus system and phage integrases. Host cell line optimization by endoglycosidase overexpression and sequence-specific genome engineering is highlighted.
Affiliation:
Helmholtz Centre for Infection Research, Structure and Function of Proteins, Inhoffenstr. 7, 38124 Braunschweig, Germany.
Citation:
Stable mammalian producer cell lines for structural biology. 2015, 32:81-90 Curr. Opin. Struct. Biol.
Journal:
Current opinion in structural biology
Issue Date:
21-Mar-2015
URI:
http://hdl.handle.net/10033/552680
DOI:
10.1016/j.sbi.2015.03.002
PubMed ID:
25804355
Type:
Article
ISSN:
1879-033X
Appears in Collections:
publications of the research group recombinant protein expression (RPEX)

Full metadata record

DC FieldValue Language
dc.contributor.authorBüssow, Konraden
dc.date.accessioned2015-05-12T14:03:28Zen
dc.date.available2015-05-12T14:03:28Zen
dc.date.issued2015-03-21en
dc.identifier.citationStable mammalian producer cell lines for structural biology. 2015, 32:81-90 Curr. Opin. Struct. Biol.en
dc.identifier.issn1879-033Xen
dc.identifier.pmid25804355en
dc.identifier.doi10.1016/j.sbi.2015.03.002en
dc.identifier.urihttp://hdl.handle.net/10033/552680en
dc.description.abstractThe mammalian cell lines HEK293 and CHO have become important expression hosts in structural biology. Generating stable mammalian cell lines remains essential for studying the function and structure of recombinant proteins, despite the emergence of highly efficient transient transfection protocols. Production with stable cell lines can be scaled up easily and high volumetric product yield can be achieved. Protein structure reports of the past two years that used stable cell lines were surveyed for this review. Well-established techniques and novel approaches for generating stable cell lines and stable cell pools are presented, including cell sorting, site-specific recombination, transposons, the Lentivirus system and phage integrases. Host cell line optimization by endoglycosidase overexpression and sequence-specific genome engineering is highlighted.en
dc.languageENGen
dc.titleStable mammalian producer cell lines for structural biology.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for Infection Research, Structure and Function of Proteins, Inhoffenstr. 7, 38124 Braunschweig, Germany.en
dc.identifier.journalCurrent opinion in structural biologyen

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