2.50
Hdl Handle:
http://hdl.handle.net/10033/553081
Title:
A rapid method for breath analysis in cystic fibrosis patients.
Authors:
Kramer, R; Sauer-Heilborn, A; Welte, T; Guzman, C A; Höfle, M G; Abraham, W-R ( 0000-0002-2850-2649 )
Abstract:
For easy handling and speed of lung diseases diagnostics, approaches based on volatile organic compounds (VOCs), including those emitted by pathogenic microorganisms, are considered but currently require considerable sampling efforts. We tested whether easy-to-handle and fast detection of lung infections is possible using solid-phase microextraction (SPME) of 100 ml of exhaled breath. An analytical procedure for the detection of VOCs from the headspace of epithelial lung cells infected with four human pathogens was developed. The feasibility of this method was tested in a cystic fibrosis (CF) outpatient clinic in vivo. Exhaled breath was extracted by SPME and analyzed by gas chromatography-mass spectrometry (GC-MS). The compositions of VOCs released in the infection model were characteristic for all individual pathogens tested. Exhaled breath of CF patients allowed clear distinction of CF patients and controls by their VOC compositions using multivariate analyses. Interestingly, the major specific VOCs detected in the exhaled breath of infected CF patients in vivo differed from those monitored during bacterial in vitro growth. SPME extraction of VOCs from 100 ml of human breath allowed the distinction between CF patients and healthy probands. Our results highlight the importance of assessing the entire pattern of VOCs instead of selected biomarkers for diagnostic purposes, as well as the need to use clinical samples to identify reliable biomarkers. This study provides the proof-of-concept for the approach using the composition of exhaled VOCs in human breath for the rapid identification of infectious agents in patients with lower respiratory tract infections.
Affiliation:
Helmholtz Centre for Infection Research, Inhoffenstraße 7, 38124 Braunschweig, Germany.
Citation:
A rapid method for breath analysis in cystic fibrosis patients. 2015, 34 (4):745-51 Eur. J. Clin. Microbiol. Infect. Dis.
Journal:
European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology
Issue Date:
Apr-2015
URI:
http://hdl.handle.net/10033/553081
DOI:
10.1007/s10096-014-2286-5
PubMed ID:
25431363
Type:
Article
Language:
en
ISSN:
1435-4373
Appears in Collections:
publications of the research group chemical microbiology (CMIK)

Full metadata record

DC FieldValue Language
dc.contributor.authorKramer, Ren
dc.contributor.authorSauer-Heilborn, Aen
dc.contributor.authorWelte, Ten
dc.contributor.authorGuzman, C Aen
dc.contributor.authorHöfle, M Gen
dc.contributor.authorAbraham, W-Ren
dc.date.accessioned2015-05-18T12:06:00Zen
dc.date.available2015-05-18T12:06:00Zen
dc.date.issued2015-04en
dc.identifier.citationA rapid method for breath analysis in cystic fibrosis patients. 2015, 34 (4):745-51 Eur. J. Clin. Microbiol. Infect. Dis.en
dc.identifier.issn1435-4373en
dc.identifier.pmid25431363en
dc.identifier.doi10.1007/s10096-014-2286-5en
dc.identifier.urihttp://hdl.handle.net/10033/553081en
dc.description.abstractFor easy handling and speed of lung diseases diagnostics, approaches based on volatile organic compounds (VOCs), including those emitted by pathogenic microorganisms, are considered but currently require considerable sampling efforts. We tested whether easy-to-handle and fast detection of lung infections is possible using solid-phase microextraction (SPME) of 100 ml of exhaled breath. An analytical procedure for the detection of VOCs from the headspace of epithelial lung cells infected with four human pathogens was developed. The feasibility of this method was tested in a cystic fibrosis (CF) outpatient clinic in vivo. Exhaled breath was extracted by SPME and analyzed by gas chromatography-mass spectrometry (GC-MS). The compositions of VOCs released in the infection model were characteristic for all individual pathogens tested. Exhaled breath of CF patients allowed clear distinction of CF patients and controls by their VOC compositions using multivariate analyses. Interestingly, the major specific VOCs detected in the exhaled breath of infected CF patients in vivo differed from those monitored during bacterial in vitro growth. SPME extraction of VOCs from 100 ml of human breath allowed the distinction between CF patients and healthy probands. Our results highlight the importance of assessing the entire pattern of VOCs instead of selected biomarkers for diagnostic purposes, as well as the need to use clinical samples to identify reliable biomarkers. This study provides the proof-of-concept for the approach using the composition of exhaled VOCs in human breath for the rapid identification of infectious agents in patients with lower respiratory tract infections.en
dc.language.isoenen
dc.titleA rapid method for breath analysis in cystic fibrosis patients.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for Infection Research, Inhoffenstraße 7, 38124 Braunschweig, Germany.en
dc.identifier.journalEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiologyen

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