Constitutive production of c-di-GMP is associated with mutations in a variant of Pseudomonas aeruginosa with altered membrane composition.

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Hdl Handle:
http://hdl.handle.net/10033/558286
Title:
Constitutive production of c-di-GMP is associated with mutations in a variant of Pseudomonas aeruginosa with altered membrane composition.
Authors:
Blanka, Andrea; Düvel, Juliane; Dötsch, Andreas; Klinkert, Birgit; Abraham, Wolf-Rainer ( 0000-0002-2850-2649 ) ; Kaever, Volkhard; Ritter, Christiane; Narberhaus, Franz; Häussler, Susanne
Abstract:
Most bacteria can form multicellular communities called biofilms on biotic and abiotic surfaces. This multicellular response to surface contact correlates with an increased resistance to various adverse environmental conditions, including those encountered during infections of the human host and exposure to antimicrobial compounds. Biofilm formation occurs when freely swimming (planktonic) cells encounter a surface, which stimulates the chemosensory-like, surface-sensing system Wsp and leads to generation of the intracellular second messenger 3',5'-cyclic-di-guanosine monophosphate (c-di-GMP). We identified adaptive mutations in a clinical small colony variant (SCV) of Pseudomonas aeruginosa and correlated their presence with self-aggregating growth behavior and an enhanced capacity to form biofilms. We present evidence that a point mutation in the 5' untranslated region of the accBC gene cluster, which encodes components of an enzyme responsible for fatty acid biosynthesis, was responsible for a stabilized mRNA structure that resulted in reduced translational efficiency and an increase in the proportion of short-chain fatty acids in the plasma membrane. We propose a model in which these changes in P. aeruginosa serve as a signal for the Wsp system to constitutively produce increased amounts of c-di-GMP and thus play a role in the regulation of adhesion-stimulated bacterial responses.
Affiliation:
Institute for Molecular Bacteriology, TWINCORE,30625 Hannover, Germany.
Citation:
Constitutive production of c-di-GMP is associated with mutations in a variant of Pseudomonas aeruginosa with altered membrane composition. 2015, 8 (372):ra36 Sci Signal
Journal:
Science signaling
Issue Date:
2015
URI:
http://hdl.handle.net/10033/558286
DOI:
10.1126/scisignal.2005943
PubMed ID:
25872871
Type:
Article
Language:
en
ISSN:
1937-9145
Appears in Collections:
publications of the departmentment of molecular bacteriology(MOBA)

Full metadata record

DC FieldValue Language
dc.contributor.authorBlanka, Andreaen
dc.contributor.authorDüvel, Julianeen
dc.contributor.authorDötsch, Andreasen
dc.contributor.authorKlinkert, Birgiten
dc.contributor.authorAbraham, Wolf-Raineren
dc.contributor.authorKaever, Volkharden
dc.contributor.authorRitter, Christianeen
dc.contributor.authorNarberhaus, Franzen
dc.contributor.authorHäussler, Susanneen
dc.date.accessioned2015-06-19T13:28:02Zen
dc.date.available2015-06-19T13:28:02Zen
dc.date.issued2015en
dc.identifier.citationConstitutive production of c-di-GMP is associated with mutations in a variant of Pseudomonas aeruginosa with altered membrane composition. 2015, 8 (372):ra36 Sci Signalen
dc.identifier.issn1937-9145en
dc.identifier.pmid25872871en
dc.identifier.doi10.1126/scisignal.2005943en
dc.identifier.urihttp://hdl.handle.net/10033/558286en
dc.description.abstractMost bacteria can form multicellular communities called biofilms on biotic and abiotic surfaces. This multicellular response to surface contact correlates with an increased resistance to various adverse environmental conditions, including those encountered during infections of the human host and exposure to antimicrobial compounds. Biofilm formation occurs when freely swimming (planktonic) cells encounter a surface, which stimulates the chemosensory-like, surface-sensing system Wsp and leads to generation of the intracellular second messenger 3',5'-cyclic-di-guanosine monophosphate (c-di-GMP). We identified adaptive mutations in a clinical small colony variant (SCV) of Pseudomonas aeruginosa and correlated their presence with self-aggregating growth behavior and an enhanced capacity to form biofilms. We present evidence that a point mutation in the 5' untranslated region of the accBC gene cluster, which encodes components of an enzyme responsible for fatty acid biosynthesis, was responsible for a stabilized mRNA structure that resulted in reduced translational efficiency and an increase in the proportion of short-chain fatty acids in the plasma membrane. We propose a model in which these changes in P. aeruginosa serve as a signal for the Wsp system to constitutively produce increased amounts of c-di-GMP and thus play a role in the regulation of adhesion-stimulated bacterial responses.en
dc.language.isoenen
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/260276en
dc.rightsopenAccessen
dc.titleConstitutive production of c-di-GMP is associated with mutations in a variant of Pseudomonas aeruginosa with altered membrane composition.en
dc.typeArticleen
dc.contributor.departmentInstitute for Molecular Bacteriology, TWINCORE,30625 Hannover, Germany.en
dc.identifier.journalScience signalingen
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