2.50
Hdl Handle:
http://hdl.handle.net/10033/558416
Title:
Biogenesis pathways of RNA guides in archaeal and bacterial CRISPR-Cas adaptive immunity.
Authors:
Charpentier, Emmanuelle; Richter, Hagen; van der Oost, John; White, Malcolm F
Abstract:
CRISPR-Cas is an RNA-mediated adaptive immune system that defends bacteria and archaea against mobile genetic elements. Short mature CRISPR RNAs (crRNAs) are key elements in the interference step of the immune pathway. A CRISPR array composed of a series of repeats interspaced by spacer sequences acquired from invading mobile genomes is transcribed as a precursor crRNA (pre-crRNA) molecule. This pre-crRNA undergoes one or two maturation steps to generate the mature crRNAs that guide CRISPR-associated (Cas) protein(s) to cognate invading genomes for their destruction. Different types of CRISPR-Cas systems have evolved distinct crRNA biogenesis pathways that implicate highly sophisticated processing mechanisms. In Types I and III CRISPR-Cas systems, a specific endoribonuclease of the Cas6 family, either standalone or in a complex with other Cas proteins, cleaves the pre-crRNA within the repeat regions. In Type II systems, the trans-acting small RNA (tracrRNA) base pairs with each repeat of the pre-crRNA to form a dual-RNA that is cleaved by the housekeeping RNase III in the presence of the protein Cas9. In this review, we present a detailed comparative analysis of pre-crRNA recognition and cleavage mechanisms involved in the biogenesis of guide crRNAs in the three CRISPR-Cas types.
Affiliation:
Helmholtz Centre for Infection Research, Inhoffenstraße 7, 38124 Braunschweig, Germany.
Citation:
Biogenesis pathways of RNA guides in archaeal and bacterial CRISPR-Cas adaptive immunity. 2015, 39 (3):428-441 FEMS Microbiol. Rev.
Journal:
FEMS microbiology reviews
Issue Date:
May-2015
URI:
http://hdl.handle.net/10033/558416
DOI:
10.1093/femsre/fuv023
PubMed ID:
25994611
Type:
Article
ISSN:
1574-6976
Appears in Collections:
publications of the department Regulation of infection

Full metadata record

DC FieldValue Language
dc.contributor.authorCharpentier, Emmanuelleen
dc.contributor.authorRichter, Hagenen
dc.contributor.authorvan der Oost, Johnen
dc.contributor.authorWhite, Malcolm Fen
dc.date.accessioned2015-06-22T13:36:21Zen
dc.date.available2015-06-22T13:36:21Zen
dc.date.issued2015-05en
dc.identifier.citationBiogenesis pathways of RNA guides in archaeal and bacterial CRISPR-Cas adaptive immunity. 2015, 39 (3):428-441 FEMS Microbiol. Rev.en
dc.identifier.issn1574-6976en
dc.identifier.pmid25994611en
dc.identifier.doi10.1093/femsre/fuv023en
dc.identifier.urihttp://hdl.handle.net/10033/558416en
dc.description.abstractCRISPR-Cas is an RNA-mediated adaptive immune system that defends bacteria and archaea against mobile genetic elements. Short mature CRISPR RNAs (crRNAs) are key elements in the interference step of the immune pathway. A CRISPR array composed of a series of repeats interspaced by spacer sequences acquired from invading mobile genomes is transcribed as a precursor crRNA (pre-crRNA) molecule. This pre-crRNA undergoes one or two maturation steps to generate the mature crRNAs that guide CRISPR-associated (Cas) protein(s) to cognate invading genomes for their destruction. Different types of CRISPR-Cas systems have evolved distinct crRNA biogenesis pathways that implicate highly sophisticated processing mechanisms. In Types I and III CRISPR-Cas systems, a specific endoribonuclease of the Cas6 family, either standalone or in a complex with other Cas proteins, cleaves the pre-crRNA within the repeat regions. In Type II systems, the trans-acting small RNA (tracrRNA) base pairs with each repeat of the pre-crRNA to form a dual-RNA that is cleaved by the housekeeping RNase III in the presence of the protein Cas9. In this review, we present a detailed comparative analysis of pre-crRNA recognition and cleavage mechanisms involved in the biogenesis of guide crRNAs in the three CRISPR-Cas types.en
dc.languageENGen
dc.titleBiogenesis pathways of RNA guides in archaeal and bacterial CRISPR-Cas adaptive immunity.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for Infection Research, Inhoffenstraße 7, 38124 Braunschweig, Germany.en
dc.identifier.journalFEMS microbiology reviewsen

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