2.50
Hdl Handle:
http://hdl.handle.net/10033/558451
Title:
Spatial distribution of Legionella pneumophila MLVA-genotypes in a drinking water system.
Authors:
Rodríguez-Martínez, Sarah; Sharaby, Yehonatan; Pecellín, Marina; Brettar, Ingrid; Höfle, Manfred; Halpern, Malka
Abstract:
Bacteria of the genus Legionella cause water-based infections, resulting in severe pneumonia. To improve our knowledge about Legionella spp. ecology, its prevalence and its relationships with environmental factors were studied. Seasonal samples were taken from both water and biofilm at seven sampling points of a small drinking water distribution system in Israel. Representative isolates were obtained from each sample and identified to the species level. Legionella pneumophila was further determined to the serotype and genotype level. High resolution genotyping of L. pneumophila isolates was achieved by Multiple-Locus Variable number of tandem repeat Analysis (MLVA). Within the studied water system, Legionella plate counts were higher in summer and highly variable even between adjacent sampling points. Legionella was present in six out of the seven selected sampling points, with counts ranging from 1.0 × 10(1) to 5.8 × 10(3) cfu/l. Water counts were significantly higher in points where Legionella was present in biofilms. The main fraction of the isolated Legionella was L. pneumophila serogroup 1. Serogroup 3 and Legionella sainthelensis were also isolated. Legionella counts were positively correlated with heterotrophic plate counts at 37 °C and negatively correlated with chlorine. Five MLVA-genotypes of L. pneumophila were identified at different buildings of the sampled area. The presence of a specific genotype, "MLVA-genotype 4", consistently co-occurred with high Legionella counts and seemed to "trigger" high Legionella counts in cold water. Our hypothesis is that both the presence of L. pneumophila in biofilm and the presence of specific genotypes, may indicate and/or even lead to high Legionella concentration in water. This observation deserves further studies in a broad range of drinking water systems to assess its potential for general use in drinking water monitoring and management.
Affiliation:
Helmholtz Centre for Infection Research, Inhoffenstraße 7, 38124 Braunschweig, Germany.
Citation:
Spatial distribution of Legionella pneumophila MLVA-genotypes in a drinking water system. 2015, 77:119-32 Water Res.
Journal:
Water research
Issue Date:
15-Jun-2015
URI:
http://hdl.handle.net/10033/558451
DOI:
10.1016/j.watres.2015.03.010
PubMed ID:
25864003
Type:
Article
Language:
en
ISSN:
1879-2448
Appears in Collections:
publications of the research group microbial diagnosis (MIDI)

Full metadata record

DC FieldValue Language
dc.contributor.authorRodríguez-Martínez, Sarahen
dc.contributor.authorSharaby, Yehonatanen
dc.contributor.authorPecellín, Marinaen
dc.contributor.authorBrettar, Ingriden
dc.contributor.authorHöfle, Manfreden
dc.contributor.authorHalpern, Malkaen
dc.date.accessioned2015-06-23T09:15:53Zen
dc.date.available2015-06-23T09:15:53Zen
dc.date.issued2015-06-15en
dc.identifier.citationSpatial distribution of Legionella pneumophila MLVA-genotypes in a drinking water system. 2015, 77:119-32 Water Res.en
dc.identifier.issn1879-2448en
dc.identifier.pmid25864003en
dc.identifier.doi10.1016/j.watres.2015.03.010en
dc.identifier.urihttp://hdl.handle.net/10033/558451en
dc.description.abstractBacteria of the genus Legionella cause water-based infections, resulting in severe pneumonia. To improve our knowledge about Legionella spp. ecology, its prevalence and its relationships with environmental factors were studied. Seasonal samples were taken from both water and biofilm at seven sampling points of a small drinking water distribution system in Israel. Representative isolates were obtained from each sample and identified to the species level. Legionella pneumophila was further determined to the serotype and genotype level. High resolution genotyping of L. pneumophila isolates was achieved by Multiple-Locus Variable number of tandem repeat Analysis (MLVA). Within the studied water system, Legionella plate counts were higher in summer and highly variable even between adjacent sampling points. Legionella was present in six out of the seven selected sampling points, with counts ranging from 1.0 × 10(1) to 5.8 × 10(3) cfu/l. Water counts were significantly higher in points where Legionella was present in biofilms. The main fraction of the isolated Legionella was L. pneumophila serogroup 1. Serogroup 3 and Legionella sainthelensis were also isolated. Legionella counts were positively correlated with heterotrophic plate counts at 37 °C and negatively correlated with chlorine. Five MLVA-genotypes of L. pneumophila were identified at different buildings of the sampled area. The presence of a specific genotype, "MLVA-genotype 4", consistently co-occurred with high Legionella counts and seemed to "trigger" high Legionella counts in cold water. Our hypothesis is that both the presence of L. pneumophila in biofilm and the presence of specific genotypes, may indicate and/or even lead to high Legionella concentration in water. This observation deserves further studies in a broad range of drinking water systems to assess its potential for general use in drinking water monitoring and management.en
dc.language.isoenen
dc.titleSpatial distribution of Legionella pneumophila MLVA-genotypes in a drinking water system.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for Infection Research, Inhoffenstraße 7, 38124 Braunschweig, Germany.en
dc.identifier.journalWater researchen

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