2.50
Hdl Handle:
http://hdl.handle.net/10033/559154
Title:
The structure of the SOLE element of oskar mRNA.
Authors:
Simon, Bernd; Masiewicz, Pawel; Ephrussi, Anne; Carlomagno, Teresa ( 0000-0002-2437-2760 )
Abstract:
mRNA localization by active transport is a regulated process that requires association of mRNPs with protein motors for transport along either the microtubule or the actin cytoskeleton. oskar mRNA localization at the posterior pole of the Drosophila oocyte requires a specific mRNA sequence, termed the SOLE, which comprises nucleotides of both exon 1 and exon 2 and is assembled upon splicing. The SOLE folds into a stem-loop structure. Both SOLE RNA and the exon junction complex (EJC) are required for oskar mRNA transport along the microtubules by kinesin. The SOLE RNA likely constitutes a recognition element for a yet unknown protein, which either belongs to the EJC or functions as a bridge between the EJC and the mRNA. Here, we determine the solution structure of the SOLE RNA by Nuclear Magnetic Resonance spectroscopy. We show that the SOLE forms a continuous helical structure, including a few noncanonical base pairs, capped by a pentanucleotide loop. The helix displays a widened major groove, which could accommodate a protein partner. In addition, the apical helical segment undergoes complex dynamics, with potential functional significance.
Affiliation:
Helmholtz Centre for Infection Research, Inhoffenstraße 7, 38124 Braunschweig, Germany.
Citation:
The structure of the SOLE element of oskar mRNA. 2015: RNA
Journal:
RNA (New York, N.Y.)
Issue Date:
18-Jun-2015
URI:
http://hdl.handle.net/10033/559154
DOI:
10.1261/rna.049601.115
PubMed ID:
26089324
Type:
Article
ISSN:
1469-9001
Appears in Collections:
Publications of the working group NMR-based structural chemistry (NBSC)

Full metadata record

DC FieldValue Language
dc.contributor.authorSimon, Bernden
dc.contributor.authorMasiewicz, Pawelen
dc.contributor.authorEphrussi, Anneen
dc.contributor.authorCarlomagno, Teresaen
dc.date.accessioned2015-07-07T13:33:47Zen
dc.date.available2015-07-07T13:33:47Zen
dc.date.issued2015-06-18en
dc.identifier.citationThe structure of the SOLE element of oskar mRNA. 2015: RNAen
dc.identifier.issn1469-9001en
dc.identifier.pmid26089324en
dc.identifier.doi10.1261/rna.049601.115en
dc.identifier.urihttp://hdl.handle.net/10033/559154en
dc.description.abstractmRNA localization by active transport is a regulated process that requires association of mRNPs with protein motors for transport along either the microtubule or the actin cytoskeleton. oskar mRNA localization at the posterior pole of the Drosophila oocyte requires a specific mRNA sequence, termed the SOLE, which comprises nucleotides of both exon 1 and exon 2 and is assembled upon splicing. The SOLE folds into a stem-loop structure. Both SOLE RNA and the exon junction complex (EJC) are required for oskar mRNA transport along the microtubules by kinesin. The SOLE RNA likely constitutes a recognition element for a yet unknown protein, which either belongs to the EJC or functions as a bridge between the EJC and the mRNA. Here, we determine the solution structure of the SOLE RNA by Nuclear Magnetic Resonance spectroscopy. We show that the SOLE forms a continuous helical structure, including a few noncanonical base pairs, capped by a pentanucleotide loop. The helix displays a widened major groove, which could accommodate a protein partner. In addition, the apical helical segment undergoes complex dynamics, with potential functional significance.en
dc.languageENGen
dc.titleThe structure of the SOLE element of oskar mRNA.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for Infection Research, Inhoffenstraße 7, 38124 Braunschweig, Germany.en
dc.identifier.journalRNA (New York, N.Y.)en

Related articles on PubMed

This item is licensed under a Creative Commons License
Creative Commons
All Items in HZI are protected by copyright, with all rights reserved, unless otherwise indicated.