2.50
Hdl Handle:
http://hdl.handle.net/10033/560745
Title:
In Vivo Efficacy of Antimicrobials against Biofilm-Producing Pseudomonas aeruginosa.
Authors:
Pawar, Vinay; Komor, Uliana; Kasnitz, Nadine; Bielecki, Piotr; Pils, Marina C; Gocht, Benjamin; Moter, Annette; Rohde, Manfred ( 0000-0003-0522-3580 ) ; Weiss, Siegfried; Häussler, Susanne
Abstract:
Patients suffering from cystic fibrosis (CF) are commonly affected by chronic Pseudomonas aeruginosa biofilm infections. This is the main cause for the high disease severity. In this study, we demonstrate that P. aeruginosa is able to efficiently colonize murine solid tumors after intravenous injection and to form biofilms in this tissue. Biofilm formation was evident by electron microscopy. Such structures could not be observed with transposon mutants, which were defective in biofilm formation. Comparative transcriptional profiling of P. aeruginosa indicated physiological similarity of the bacteria in the murine tumor model and the CF lung. The efficacy of currently available antibiotics for treatment of P. aeruginosa-infected CF lungs, such as ciprofloxacin, colistin, and tobramycin, could be tested in the tumor model. We found that clinically recommended doses of these antibiotics were unable to eliminate wild-type P. aeruginosa PA14 while being effective against biofilm-defective mutants. However, colistin-tobramycin combination therapy significantly reduced the number of P. aeruginosa PA14 cells in tumors at lower concentrations. Hence, we present a versatile experimental system that is providing a platform to test approved and newly developed antibiofilm compounds.
Affiliation:
Molecular Bacteriology, Twincore, Centre for Clinical and Experimental Infection Research, Hannover, Germany.
Citation:
In Vivo Efficacy of Antimicrobials against Biofilm-Producing Pseudomonas aeruginosa. 2015, 59 (8):4974-81 Antimicrob. Agents Chemother.
Journal:
Antimicrobial agents and chemotherapy
Issue Date:
Aug-2015
URI:
http://hdl.handle.net/10033/560745
DOI:
10.1128/AAC.00194-15
PubMed ID:
26055372
Type:
Article
Language:
en
ISSN:
1098-6596
Appears in Collections:
publications of the departmentment of molecular bacteriology(MOBA)

Full metadata record

DC FieldValue Language
dc.contributor.authorPawar, Vinayen
dc.contributor.authorKomor, Ulianaen
dc.contributor.authorKasnitz, Nadineen
dc.contributor.authorBielecki, Piotren
dc.contributor.authorPils, Marina Cen
dc.contributor.authorGocht, Benjaminen
dc.contributor.authorMoter, Annetteen
dc.contributor.authorRohde, Manfreden
dc.contributor.authorWeiss, Siegfrieden
dc.contributor.authorHäussler, Susanneen
dc.date.accessioned2015-07-20T13:07:42Zen
dc.date.available2015-07-20T13:07:42Zen
dc.date.issued2015-08en
dc.identifier.citationIn Vivo Efficacy of Antimicrobials against Biofilm-Producing Pseudomonas aeruginosa. 2015, 59 (8):4974-81 Antimicrob. Agents Chemother.en
dc.identifier.issn1098-6596en
dc.identifier.pmid26055372en
dc.identifier.doi10.1128/AAC.00194-15en
dc.identifier.urihttp://hdl.handle.net/10033/560745en
dc.description.abstractPatients suffering from cystic fibrosis (CF) are commonly affected by chronic Pseudomonas aeruginosa biofilm infections. This is the main cause for the high disease severity. In this study, we demonstrate that P. aeruginosa is able to efficiently colonize murine solid tumors after intravenous injection and to form biofilms in this tissue. Biofilm formation was evident by electron microscopy. Such structures could not be observed with transposon mutants, which were defective in biofilm formation. Comparative transcriptional profiling of P. aeruginosa indicated physiological similarity of the bacteria in the murine tumor model and the CF lung. The efficacy of currently available antibiotics for treatment of P. aeruginosa-infected CF lungs, such as ciprofloxacin, colistin, and tobramycin, could be tested in the tumor model. We found that clinically recommended doses of these antibiotics were unable to eliminate wild-type P. aeruginosa PA14 while being effective against biofilm-defective mutants. However, colistin-tobramycin combination therapy significantly reduced the number of P. aeruginosa PA14 cells in tumors at lower concentrations. Hence, we present a versatile experimental system that is providing a platform to test approved and newly developed antibiofilm compounds.en
dc.language.isoenen
dc.titleIn Vivo Efficacy of Antimicrobials against Biofilm-Producing Pseudomonas aeruginosa.en
dc.typeArticleen
dc.contributor.departmentMolecular Bacteriology, Twincore, Centre for Clinical and Experimental Infection Research, Hannover, Germany.en
dc.identifier.journalAntimicrobial agents and chemotherapyen

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