2.50
Hdl Handle:
http://hdl.handle.net/10033/564203
Title:
Stool metatranscriptomics: A technical guideline for mRNA stabilisation and isolation.
Authors:
Reck, Michael; Tomasch, Jürgen; Deng, Zhiluo; Jarek, Michael; Husemann, Peter; Wagner-Döbler, Irene
Abstract:
The complex microbiome of the gut has an enormous impact on human health. Analysis of the transcriptional activity of microorganisms through mRNA sequencing (metatranscriptomics) opens a completely new window into their activity in vivo, but it is highly challenging due to numerous technical and bioinformatical obstacles. Here we present an optimized pipeline for extraction of high quality mRNA from stool samples.
Affiliation:
Helmholtz Centre for Infection Research, Inhoffenstraße 7, 38124 Braunschweig, Germany.
Citation:
Stool metatranscriptomics: A technical guideline for mRNA stabilisation and isolation. 2015, 16:494 BMC Genomics
Journal:
BMC genomics
Issue Date:
2015
URI:
http://hdl.handle.net/10033/564203
DOI:
10.1186/s12864-015-1694-y
PubMed ID:
26140923
Type:
Article
Language:
en
ISSN:
1471-2164
Appears in Collections:
collections of the research group microbial communication (KOM)

Full metadata record

DC FieldValue Language
dc.contributor.authorReck, Michaelen
dc.contributor.authorTomasch, Jürgenen
dc.contributor.authorDeng, Zhiluoen
dc.contributor.authorJarek, Michaelen
dc.contributor.authorHusemann, Peteren
dc.contributor.authorWagner-Döbler, Ireneen
dc.date.accessioned2015-08-03T13:36:17Zen
dc.date.available2015-08-03T13:36:17Zen
dc.date.issued2015en
dc.identifier.citationStool metatranscriptomics: A technical guideline for mRNA stabilisation and isolation. 2015, 16:494 BMC Genomicsen
dc.identifier.issn1471-2164en
dc.identifier.pmid26140923en
dc.identifier.doi10.1186/s12864-015-1694-yen
dc.identifier.urihttp://hdl.handle.net/10033/564203en
dc.description.abstractThe complex microbiome of the gut has an enormous impact on human health. Analysis of the transcriptional activity of microorganisms through mRNA sequencing (metatranscriptomics) opens a completely new window into their activity in vivo, but it is highly challenging due to numerous technical and bioinformatical obstacles. Here we present an optimized pipeline for extraction of high quality mRNA from stool samples.en
dc.language.isoenen
dc.titleStool metatranscriptomics: A technical guideline for mRNA stabilisation and isolation.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for Infection Research, Inhoffenstraße 7, 38124 Braunschweig, Germany.en
dc.identifier.journalBMC genomicsen

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