miR-20a inhibits TCR-mediated signaling and cytokine production in human naïve CD4+ T cells.

2.50
Hdl Handle:
http://hdl.handle.net/10033/578625
Title:
miR-20a inhibits TCR-mediated signaling and cytokine production in human naïve CD4+ T cells.
Authors:
Reddycherla, Amarendra V; Meinert, Ines; Reinhold, Annegret; Reinhold, Dirk; Schraven, Burkhart; Simeoni, Luca
Abstract:
Upon TCR stimulation by peptide-MHC complexes, CD4+ T cells undergo activation and proliferation. This process will ultimately culminate in T-cell differentiation and the acquisition of effector functions. The production of specific cytokines by differentiated CD4+ T cells is crucial for the generation of the appropriate immune response. Altered CD4+ T-cell activation and cytokine production result in chronic inflammatory conditions and autoimmune disorders. miRNAs have been shown to be important regulators of T-cell biology. In this study, we have focused our investigation on miR-20a, a member of the miR-17-92 cluster, whose expression is decreased in patients suffering from multiple sclerosis. We have found that miR-20a is rapidly induced upon TCR-triggering in primary human naïve CD4+ T cells and that its transcription is regulated in a Erk-, NF-κB-, and Ca++-dependent manner. We have further shown that overexpression of miR-20a inhibits TCR-mediated signaling but not the proliferation of primary human naïve CD4+ T cells. However, miR-20a overexpression strongly suppresses IL-10 secretion and moderately decreases IL-2, IL-6 and IL8 production, which are crucial regulators of inflammatory responses. Our study suggests that miR-20a is a new player in the regulation of TCR signaling strength and cytokine production.
Affiliation:
Helmholtz Centre for infection research, Inhoffenstr. 7, D-38124 Braunschweig, Germany.
Citation:
miR-20a inhibits TCR-mediated signaling and cytokine production in human naïve CD4+ T cells. 2015, 10 (4):e0125311 PLoS ONE
Journal:
PloS one
Issue Date:
2015
URI:
http://hdl.handle.net/10033/578625
DOI:
10.1371/journal.pone.0125311
PubMed ID:
25884400
Type:
Article
Language:
en
ISSN:
1932-6203
Appears in Collections:
publications of the department of immune control (IMMK)

Full metadata record

DC FieldValue Language
dc.contributor.authorReddycherla, Amarendra Ven
dc.contributor.authorMeinert, Inesen
dc.contributor.authorReinhold, Annegreten
dc.contributor.authorReinhold, Dirken
dc.contributor.authorSchraven, Burkharten
dc.contributor.authorSimeoni, Lucaen
dc.date.accessioned2015-09-23T12:03:29Zen
dc.date.available2015-09-23T12:03:29Zen
dc.date.issued2015en
dc.identifier.citationmiR-20a inhibits TCR-mediated signaling and cytokine production in human naïve CD4+ T cells. 2015, 10 (4):e0125311 PLoS ONEen
dc.identifier.issn1932-6203en
dc.identifier.pmid25884400en
dc.identifier.doi10.1371/journal.pone.0125311en
dc.identifier.urihttp://hdl.handle.net/10033/578625en
dc.description.abstractUpon TCR stimulation by peptide-MHC complexes, CD4+ T cells undergo activation and proliferation. This process will ultimately culminate in T-cell differentiation and the acquisition of effector functions. The production of specific cytokines by differentiated CD4+ T cells is crucial for the generation of the appropriate immune response. Altered CD4+ T-cell activation and cytokine production result in chronic inflammatory conditions and autoimmune disorders. miRNAs have been shown to be important regulators of T-cell biology. In this study, we have focused our investigation on miR-20a, a member of the miR-17-92 cluster, whose expression is decreased in patients suffering from multiple sclerosis. We have found that miR-20a is rapidly induced upon TCR-triggering in primary human naïve CD4+ T cells and that its transcription is regulated in a Erk-, NF-κB-, and Ca++-dependent manner. We have further shown that overexpression of miR-20a inhibits TCR-mediated signaling but not the proliferation of primary human naïve CD4+ T cells. However, miR-20a overexpression strongly suppresses IL-10 secretion and moderately decreases IL-2, IL-6 and IL8 production, which are crucial regulators of inflammatory responses. Our study suggests that miR-20a is a new player in the regulation of TCR signaling strength and cytokine production.en
dc.language.isoenen
dc.titlemiR-20a inhibits TCR-mediated signaling and cytokine production in human naïve CD4+ T cells.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection research, Inhoffenstr. 7, D-38124 Braunschweig, Germany.en
dc.identifier.journalPloS oneen

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