Extracellular milieu grossly alters pathogen-specific immune response of mammary epithelial cells.
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Issue Date
2015
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Show full item recordAbstract
Considerably divergent data have been published from attempts to model the E. coli vs. S. aureus specific immune reaction of the udder using primary cultures of bovine mammary epithelial cells from cows (pbMEC). Some groups reported a swift, strong and transient inflammatory response against challenges with E. coli and only a weak and retarded response against S. aureus, in agreement with the respective reaction of the udder. Others found almost the reverse. Presence or absence of fetal calf serum distinguished the experimental setting between both groups. We examined here if this causes the divergent reaction of the pbMEC towards both pathogen species. We challenged pbMEC with proteins from heat killed E. coli or S. aureus pathogens or purified TLR2 and TLR4 ligands. The stimuli were applied in normal growth medium with (SM10) or without (SM0) 10% fetal calf serum, or in the basal medium supplemented with 10 mg/ml milk proteins (SM Milk).Citation
Extracellular milieu grossly alters pathogen-specific immune response of mammary epithelial cells. 2015, 11:172 BMC Vet. Res.Affiliation
Helmholtz Center for Infection ResearchJournal
BMC veterinary researchPubMed ID
26219462Type
ArticleLanguage
enISSN
1746-6148ae974a485f413a2113503eed53cd6c53
10.1186/s12917-015-0489-3
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