Cross talk between the response regulators PhoB and TctD allows for the integration of diverse environmental signals in Pseudomonas aeruginosa.

2.50
Hdl Handle:
http://hdl.handle.net/10033/581417
Title:
Cross talk between the response regulators PhoB and TctD allows for the integration of diverse environmental signals in Pseudomonas aeruginosa.
Authors:
Bielecki, Piotr; Jensen, Vanessa; Schulze, Wiebke; Gödeke, Julia; Strehmel, Janine; Eckweiler, Denitsa; Nicolai, Tanja; Bielecka, Agata; Wille, Thorsten; Gerlach, Roman G; Häussler, Susanne
Abstract:
Two-component systems (TCS) serve as stimulus-response coupling mechanisms to allow organisms to adapt to a variety of environmental conditions. The opportunistic pathogen Pseudomonas aeruginosa encodes for more than 100 TCS components. To avoid unwanted cross-talk, signaling cascades are very specific, with one sensor talking to its cognate response regulator (RR). However, cross-regulation may provide means to integrate different environmental stimuli into a harmonized output response. By applying a split luciferase complementation assay, we identified a functional interaction of two RRs of the OmpR/PhoB subfamily, namely PhoB and TctD in P. aeruginosa. Transcriptional profiling, ChIP-seq analysis and a global motif scan uncovered the regulons of the two RRs as well as a quadripartite binding motif in six promoter regions. Phosphate limitation resulted in PhoB-dependent expression of the downstream genes, whereas the presence of TctD counteracted this activation. Thus, the integration of two important environmental signals e.g. phosphate availability and the carbon source are achieved by a titration of the relative amounts of two phosphorylated RRs that inversely regulate a common subset of genes. In conclusion, our results on the PhoB and TctD mediated two-component signal transduction pathways exemplify how P. aeruginosa may exploit cross-regulation to adapt bacterial behavior to complex environments.
Affiliation:
HZI-Helmholzzentrum für Infektionsforschung
Citation:
Cross talk between the response regulators PhoB and TctD allows for the integration of diverse environmental signals in Pseudomonas aeruginosa. 2015, 43 (13):6413-25 Nucleic Acids Res.
Journal:
Nucleic acids research
Issue Date:
27-Jul-2015
URI:
http://hdl.handle.net/10033/581417
DOI:
10.1093/nar/gkv599
PubMed ID:
26082498
Type:
Article
Language:
en
ISSN:
1362-4962
Appears in Collections:
publications of the departmentment of molecular bacteriology(MOBA)

Full metadata record

DC FieldValue Language
dc.contributor.authorBielecki, Piotren
dc.contributor.authorJensen, Vanessaen
dc.contributor.authorSchulze, Wiebkeen
dc.contributor.authorGödeke, Juliaen
dc.contributor.authorStrehmel, Janineen
dc.contributor.authorEckweiler, Denitsaen
dc.contributor.authorNicolai, Tanjaen
dc.contributor.authorBielecka, Agataen
dc.contributor.authorWille, Thorstenen
dc.contributor.authorGerlach, Roman Gen
dc.contributor.authorHäussler, Susanneen
dc.date.accessioned2015-10-29T11:59:02Zen
dc.date.available2015-10-29T11:59:02Zen
dc.date.issued2015-07-27en
dc.identifier.citationCross talk between the response regulators PhoB and TctD allows for the integration of diverse environmental signals in Pseudomonas aeruginosa. 2015, 43 (13):6413-25 Nucleic Acids Res.en
dc.identifier.issn1362-4962en
dc.identifier.pmid26082498en
dc.identifier.doi10.1093/nar/gkv599en
dc.identifier.urihttp://hdl.handle.net/10033/581417en
dc.description.abstractTwo-component systems (TCS) serve as stimulus-response coupling mechanisms to allow organisms to adapt to a variety of environmental conditions. The opportunistic pathogen Pseudomonas aeruginosa encodes for more than 100 TCS components. To avoid unwanted cross-talk, signaling cascades are very specific, with one sensor talking to its cognate response regulator (RR). However, cross-regulation may provide means to integrate different environmental stimuli into a harmonized output response. By applying a split luciferase complementation assay, we identified a functional interaction of two RRs of the OmpR/PhoB subfamily, namely PhoB and TctD in P. aeruginosa. Transcriptional profiling, ChIP-seq analysis and a global motif scan uncovered the regulons of the two RRs as well as a quadripartite binding motif in six promoter regions. Phosphate limitation resulted in PhoB-dependent expression of the downstream genes, whereas the presence of TctD counteracted this activation. Thus, the integration of two important environmental signals e.g. phosphate availability and the carbon source are achieved by a titration of the relative amounts of two phosphorylated RRs that inversely regulate a common subset of genes. In conclusion, our results on the PhoB and TctD mediated two-component signal transduction pathways exemplify how P. aeruginosa may exploit cross-regulation to adapt bacterial behavior to complex environments.en
dc.language.isoenen
dc.subject.meshBacterial Proteinsen
dc.subject.meshBase Sequenceen
dc.subject.meshBinding Sitesen
dc.subject.meshConsensus Sequenceen
dc.subject.meshDNA-Binding Proteinsen
dc.subject.meshGene Expression Regulation, Bacterialen
dc.subject.meshLuciferasesen
dc.subject.meshPromoter Regions, Geneticen
dc.subject.meshPseudomonas aeruginosaen
dc.subject.meshRegulonen
dc.subject.meshSignal Transductionen
dc.subject.meshTranscription, Geneticen
dc.titleCross talk between the response regulators PhoB and TctD allows for the integration of diverse environmental signals in Pseudomonas aeruginosa.en
dc.typeArticleen
dc.contributor.departmentHZI-Helmholzzentrum für Infektionsforschungen
dc.identifier.journalNucleic acids researchen
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