Endophytic Streptomyces in the traditional medicinal plant Arnica montana L.: secondary metabolites and biological activity.

2.50
Hdl Handle:
http://hdl.handle.net/10033/582601
Title:
Endophytic Streptomyces in the traditional medicinal plant Arnica montana L.: secondary metabolites and biological activity.
Authors:
Wardecki, Tina; Brötz, Elke; De Ford, Christian; von Loewenich, Friederike D; Rebets, Yuriy; Tokovenko, Bogdan; Luzhetskyy, Andriy ( 0000-0001-6497-0047 ) ; Merfort, Irmgard
Abstract:
Arnica montana L. is a medical plant of the Asteraceae family and grows preferably on nutrient poor soils in mountainous environments. Such surroundings are known to make plants dependent on symbiosis with other organisms. Up to now only arbuscular mycorrhizal fungi were found to act as endophytic symbiosis partners for A. montana. Here we identified five Streptomyces strains, microorganisms also known to occur as endophytes in plants and to produce a huge variety of active secondary metabolites, as inhabitants of A. montana. The secondary metabolite spectrum of these strains does not contain sesquiterpene lactones, but consists of the glutarimide antibiotics cycloheximide and actiphenol as well as the diketopiperazines cyclo-prolyl-valyl, cyclo-prolyl-isoleucyl, cyclo-prolyl-leucyl and cyclo-prolyl-phenylalanyl. Notably, genome analysis of one strain was performed and indicated a huge genome size with a high number of natural products gene clusters among which genes for cycloheximide production were detected. Only weak activity against the Gram-positive bacterium Staphylococcus aureus was revealed, but the extracts showed a marked cytotoxic activity as well as an antifungal activity against Candida parapsilosis and Fusarium verticillioides. Altogether, our results provide evidence that A. montana and its endophytic Streptomyces benefit from each other by completing their protection against competitors and pathogens and by exchanging plant growth promoting signals with nutrients.
Affiliation:
Helmholtz Institute for Pharmaceutical Research Saarland,Saarbrücken, Saarland 66123, Germany.
Citation:
Endophytic Streptomyces in the traditional medicinal plant Arnica montana L.: secondary metabolites and biological activity. 2015, 108 (2):391-402 Antonie Van Leeuwenhoek
Journal:
Antonie van Leeuwenhoek
Issue Date:
Aug-2015
URI:
http://hdl.handle.net/10033/582601
DOI:
10.1007/s10482-015-0492-5
PubMed ID:
26036671
Type:
Article
Language:
en
ISSN:
1572-9699
Appears in Collections:
publications of the research group metabolic engineering of Actinomycetes ([HIPS]AMEG)

Full metadata record

DC FieldValue Language
dc.contributor.authorWardecki, Tinaen
dc.contributor.authorBrötz, Elkeen
dc.contributor.authorDe Ford, Christianen
dc.contributor.authorvon Loewenich, Friederike Den
dc.contributor.authorRebets, Yuriyen
dc.contributor.authorTokovenko, Bogdanen
dc.contributor.authorLuzhetskyy, Andriyen
dc.contributor.authorMerfort, Irmgarden
dc.date.accessioned2015-11-24T12:46:26Zen
dc.date.available2015-11-24T12:46:26Zen
dc.date.issued2015-08en
dc.identifier.citationEndophytic Streptomyces in the traditional medicinal plant Arnica montana L.: secondary metabolites and biological activity. 2015, 108 (2):391-402 Antonie Van Leeuwenhoeken
dc.identifier.issn1572-9699en
dc.identifier.pmid26036671en
dc.identifier.doi10.1007/s10482-015-0492-5en
dc.identifier.urihttp://hdl.handle.net/10033/582601en
dc.description.abstractArnica montana L. is a medical plant of the Asteraceae family and grows preferably on nutrient poor soils in mountainous environments. Such surroundings are known to make plants dependent on symbiosis with other organisms. Up to now only arbuscular mycorrhizal fungi were found to act as endophytic symbiosis partners for A. montana. Here we identified five Streptomyces strains, microorganisms also known to occur as endophytes in plants and to produce a huge variety of active secondary metabolites, as inhabitants of A. montana. The secondary metabolite spectrum of these strains does not contain sesquiterpene lactones, but consists of the glutarimide antibiotics cycloheximide and actiphenol as well as the diketopiperazines cyclo-prolyl-valyl, cyclo-prolyl-isoleucyl, cyclo-prolyl-leucyl and cyclo-prolyl-phenylalanyl. Notably, genome analysis of one strain was performed and indicated a huge genome size with a high number of natural products gene clusters among which genes for cycloheximide production were detected. Only weak activity against the Gram-positive bacterium Staphylococcus aureus was revealed, but the extracts showed a marked cytotoxic activity as well as an antifungal activity against Candida parapsilosis and Fusarium verticillioides. Altogether, our results provide evidence that A. montana and its endophytic Streptomyces benefit from each other by completing their protection against competitors and pathogens and by exchanging plant growth promoting signals with nutrients.en
dc.language.isoenen
dc.titleEndophytic Streptomyces in the traditional medicinal plant Arnica montana L.: secondary metabolites and biological activity.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Institute for Pharmaceutical Research Saarland,Saarbrücken, Saarland 66123, Germany.en
dc.identifier.journalAntonie van Leeuwenhoeken

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