Murine cytomegalovirus infection via the intranasal route offers a robust model of immunity upon mucosal CMV infection.

2.50
Hdl Handle:
http://hdl.handle.net/10033/582776
Title:
Murine cytomegalovirus infection via the intranasal route offers a robust model of immunity upon mucosal CMV infection.
Authors:
Oduro, Jennifer D; Redeker, Anke; Lemmermann, Niels A W; Ebermann, Linda; Marandu, Thomas F; Dekhtiarenko, Iryna; Holzki, Julia K; Busch, Dirk; Arens, Ramon; Cicin-Sain, Luka ( 0000-0003-3978-778X )
Abstract:
Cytomegalovirus (CMV) is a ubiquitous virus, causing the most common congenital infection in humans, yet a vaccine against this virus is not available. The experimental study of immunity against CMV in animal models of infection, such as the infection of mice with the mouse CMV (MCMV), has relied on systemic intraperitoneal infection protocols, although the infection naturally transmits by mucosal routes via body fluids containing CMV. To characterize the biology of infections by mucosal routes, we have compared the kinetics of virus replication, the latent viral load, and CD8 T cell responses in lymphoid organs upon experimental intranasal and intragastric infection to intraperitoneal infection of two unrelated mouse strains. We have observed that intranasal infection induces robust and persistent virus replication in lungs and salivary glands, but a poor one in the spleen. CD8 T cell responses were somewhat weaker than upon intraperitoneal infection, but showed similar kinetic profiles and phenotypes of antigen-specific cells. On the other hand, intragastric infection resulted in abortive or poor virus replication in all tested organs, and poor T cell responses to the virus, especially at late times after infection. Consistent with the T cell kinetics, the MCMV latent load was high in the lungs, but low in the spleen of intranasally infected mice and lowest in all tested organs upon intragastric infection. In conclusion, we show here that intranasal, but not intragastric infection of mice with MCMV represents a robust model to study short and long-term biology of CMV infection by a mucosal route.
Affiliation:
Helmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.
Citation:
Murine cytomegalovirus infection via the intranasal route offers a robust model of immunity upon mucosal CMV infection. 2015: J. Gen. Virol.
Journal:
The Journal of general virology
Issue Date:
10-Nov-2015
URI:
http://hdl.handle.net/10033/582776
DOI:
10.1099/jgv.0.000339
PubMed ID:
26555192
Type:
Article
ISSN:
1465-2099
Appears in Collections:
publications of the research group immune aging and chronic infections (IMCI)

Full metadata record

DC FieldValue Language
dc.contributor.authorOduro, Jennifer Den
dc.contributor.authorRedeker, Ankeen
dc.contributor.authorLemmermann, Niels A Wen
dc.contributor.authorEbermann, Lindaen
dc.contributor.authorMarandu, Thomas Fen
dc.contributor.authorDekhtiarenko, Irynaen
dc.contributor.authorHolzki, Julia Ken
dc.contributor.authorBusch, Dirken
dc.contributor.authorArens, Ramonen
dc.contributor.authorCicin-Sain, Lukaen
dc.date.accessioned2015-11-27T10:04:27Zen
dc.date.available2015-11-27T10:04:27Zen
dc.date.issued2015-11-10en
dc.identifier.citationMurine cytomegalovirus infection via the intranasal route offers a robust model of immunity upon mucosal CMV infection. 2015: J. Gen. Virol.en
dc.identifier.issn1465-2099en
dc.identifier.pmid26555192en
dc.identifier.doi10.1099/jgv.0.000339en
dc.identifier.urihttp://hdl.handle.net/10033/582776en
dc.description.abstractCytomegalovirus (CMV) is a ubiquitous virus, causing the most common congenital infection in humans, yet a vaccine against this virus is not available. The experimental study of immunity against CMV in animal models of infection, such as the infection of mice with the mouse CMV (MCMV), has relied on systemic intraperitoneal infection protocols, although the infection naturally transmits by mucosal routes via body fluids containing CMV. To characterize the biology of infections by mucosal routes, we have compared the kinetics of virus replication, the latent viral load, and CD8 T cell responses in lymphoid organs upon experimental intranasal and intragastric infection to intraperitoneal infection of two unrelated mouse strains. We have observed that intranasal infection induces robust and persistent virus replication in lungs and salivary glands, but a poor one in the spleen. CD8 T cell responses were somewhat weaker than upon intraperitoneal infection, but showed similar kinetic profiles and phenotypes of antigen-specific cells. On the other hand, intragastric infection resulted in abortive or poor virus replication in all tested organs, and poor T cell responses to the virus, especially at late times after infection. Consistent with the T cell kinetics, the MCMV latent load was high in the lungs, but low in the spleen of intranasally infected mice and lowest in all tested organs upon intragastric infection. In conclusion, we show here that intranasal, but not intragastric infection of mice with MCMV represents a robust model to study short and long-term biology of CMV infection by a mucosal route.en
dc.languageENGen
dc.relationnfo:eu-repo/grantAgreement/EC/FP7/ERC-2010- 26093en
dc.rightsembargoedAccessen
dc.titleMurine cytomegalovirus infection via the intranasal route offers a robust model of immunity upon mucosal CMV infection.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.en
dc.identifier.journalThe Journal of general virologyen
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