Testing the utility of site-specific recombinases for manipulations of genome of moenomycin producer Streptomyces ghanaensis ATCC14672.

2.50
Hdl Handle:
http://hdl.handle.net/10033/583485
Title:
Testing the utility of site-specific recombinases for manipulations of genome of moenomycin producer Streptomyces ghanaensis ATCC14672.
Authors:
Lopatniuk, M; Ostash, B ( 0000-0001-5904-5957 ) ; Makitrynskyy, R; Walker, S; Luzhetskyy, A ( 0000-0001-6497-0047 ) ; Fedorenko, V ( 0000-0002-7672-1897 )
Abstract:
Streptomyces ghanaensis ATCC14672 is the producer of phosphoglycolipid antibiotics moenomycins that for almost 40 years were used worldwide as an animal feed additive. As the use of moenomycins narrows down (due to bans in the EU and some other countries), it opens the opportunity to develop much-needed antibiotics against Gram-positive human pathogens, such as cocci. It is desirable to develop ATCC14672 strains accumulating only certain members of moenomycin family which would facilitate their purification, analysis and/or chemical modification. Here we tested site-specific recombinases (SSRs) as a tool to manipulate the genome of ATCC14672 and to achieve aforementioned goals. We show that of three SSRs tested - Cre, Dre, and Flp - the first two efficiently catalyzed recombination reactions, while Flp showed no activity in ATCC14672 cells. Cre recombinase can be reused at least three times to modify ATCC14672 genome without detrimental effects, such as large-scale inversions or deletions. Properties of the generated strains and SSRs are discussed.
Affiliation:
Helmholtz Institute for Pharmaceutical Research Saarland,Saarbrücken, Saarland 66123, Germany.
Citation:
Testing the utility of site-specific recombinases for manipulations of genome of moenomycin producer Streptomyces ghanaensis ATCC14672. 2015, 56 (4):547-50 J. Appl. Genet.
Journal:
Journal of applied genetics
Issue Date:
Nov-2015
URI:
http://hdl.handle.net/10033/583485
DOI:
10.1007/s13353-015-0283-8
PubMed ID:
25801470
Type:
Article
Language:
en
ISSN:
2190-3883
Appears in Collections:
publications of the research group metabolic engineering of Actinomycetes ([HIPS]AMEG)

Full metadata record

DC FieldValue Language
dc.contributor.authorLopatniuk, Men
dc.contributor.authorOstash, Ben
dc.contributor.authorMakitrynskyy, Ren
dc.contributor.authorWalker, Sen
dc.contributor.authorLuzhetskyy, Aen
dc.contributor.authorFedorenko, Ven
dc.date.accessioned2015-12-09T13:27:03Zen
dc.date.available2015-12-09T13:27:03Zen
dc.date.issued2015-11en
dc.identifier.citationTesting the utility of site-specific recombinases for manipulations of genome of moenomycin producer Streptomyces ghanaensis ATCC14672. 2015, 56 (4):547-50 J. Appl. Genet.en
dc.identifier.issn2190-3883en
dc.identifier.pmid25801470en
dc.identifier.doi10.1007/s13353-015-0283-8en
dc.identifier.urihttp://hdl.handle.net/10033/583485en
dc.description.abstractStreptomyces ghanaensis ATCC14672 is the producer of phosphoglycolipid antibiotics moenomycins that for almost 40 years were used worldwide as an animal feed additive. As the use of moenomycins narrows down (due to bans in the EU and some other countries), it opens the opportunity to develop much-needed antibiotics against Gram-positive human pathogens, such as cocci. It is desirable to develop ATCC14672 strains accumulating only certain members of moenomycin family which would facilitate their purification, analysis and/or chemical modification. Here we tested site-specific recombinases (SSRs) as a tool to manipulate the genome of ATCC14672 and to achieve aforementioned goals. We show that of three SSRs tested - Cre, Dre, and Flp - the first two efficiently catalyzed recombination reactions, while Flp showed no activity in ATCC14672 cells. Cre recombinase can be reused at least three times to modify ATCC14672 genome without detrimental effects, such as large-scale inversions or deletions. Properties of the generated strains and SSRs are discussed.en
dc.language.isoenen
dc.titleTesting the utility of site-specific recombinases for manipulations of genome of moenomycin producer Streptomyces ghanaensis ATCC14672.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Institute for Pharmaceutical Research Saarland,Saarbrücken, Saarland 66123, Germany.en
dc.identifier.journalJournal of applied geneticsen

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