Expression and function of the epithelial sodium channel δ-subunit in human respiratory epithelial cells in vitro.

2.50
Hdl Handle:
http://hdl.handle.net/10033/592820
Title:
Expression and function of the epithelial sodium channel δ-subunit in human respiratory epithelial cells in vitro.
Authors:
Schwagerus, Elena; Sladek, Svenja; Buckley, Stephen T; Armas-Capote, Natalia; Alvarez de la Rosa, Diego; Harvey, Brian J; Fischer, Horst; Illek, Beate; Huwer, Hanno; Schneider-Daum, Nicole; Lehr, Claus-Michael; Ehrhardt, Carsten
Abstract:
Using human airway epithelial cell lines (i.e. NCI-H441 and Calu-3) as well as human alveolar epithelial type I-like (ATI) cells in primary culture, we studied the contribution of the epithelial sodium channel δ-subunit (δ-ENaC) to transepithelial sodium transport in human lung in vitro. Endogenous δ-ENaC protein was present in all three cell types tested; however, protein abundance was low, and no expression was detected in the apical cell membrane of these cells. Similarly, known modulators of δ-ENaC activity, such as capsazepine and icilin (activators) and Evans blue (inhibitor), did not show effects on short-circuit current (I SC), suggesting that δ-ENaC is not involved in the modulation of transcellular sodium absorption in NCI-H441 cell monolayers. Over-expression of δ-ENaC in NCI-H441 cells resulted in detectable protein expression in the apical cell membrane, as well as capsazepine and icilin-stimulated increases in I SC that were effectively blocked by Evans blue and that were consistent with δ-ENaC activation and inhibition, respectively. Consequently, these observations suggest that δ-ENaC expression is low in NCI-H441, Calu-3, and ATI cells and does not contribute to transepithelial sodium absorption.
Affiliation:
Helmholtz Institute for Pharmaceutical Research Saarland, 66123 Saarbrücken, Germany
Citation:
Expression and function of the epithelial sodium channel δ-subunit in human respiratory epithelial cells in vitro. 2015, 467 (11):2257-73 Pflugers Arch.
Journal:
Pflügers Archiv : European journal of physiology
Issue Date:
Nov-2015
URI:
http://hdl.handle.net/10033/592820
DOI:
10.1007/s00424-015-1693-5
PubMed ID:
25677639
Type:
Article
Language:
en
ISSN:
1432-2013
Appears in Collections:
publications of the department drug delivery ([TC] DDEL)

Full metadata record

DC FieldValue Language
dc.contributor.authorSchwagerus, Elenaen
dc.contributor.authorSladek, Svenjaen
dc.contributor.authorBuckley, Stephen Ten
dc.contributor.authorArmas-Capote, Nataliaen
dc.contributor.authorAlvarez de la Rosa, Diegoen
dc.contributor.authorHarvey, Brian Jen
dc.contributor.authorFischer, Horsten
dc.contributor.authorIllek, Beateen
dc.contributor.authorHuwer, Hannoen
dc.contributor.authorSchneider-Daum, Nicoleen
dc.contributor.authorLehr, Claus-Michaelen
dc.contributor.authorEhrhardt, Carstenen
dc.date.accessioned2016-01-05T09:05:20Zen
dc.date.available2016-01-05T09:05:20Zen
dc.date.issued2015-11en
dc.identifier.citationExpression and function of the epithelial sodium channel δ-subunit in human respiratory epithelial cells in vitro. 2015, 467 (11):2257-73 Pflugers Arch.en
dc.identifier.issn1432-2013en
dc.identifier.pmid25677639en
dc.identifier.doi10.1007/s00424-015-1693-5en
dc.identifier.urihttp://hdl.handle.net/10033/592820en
dc.description.abstractUsing human airway epithelial cell lines (i.e. NCI-H441 and Calu-3) as well as human alveolar epithelial type I-like (ATI) cells in primary culture, we studied the contribution of the epithelial sodium channel δ-subunit (δ-ENaC) to transepithelial sodium transport in human lung in vitro. Endogenous δ-ENaC protein was present in all three cell types tested; however, protein abundance was low, and no expression was detected in the apical cell membrane of these cells. Similarly, known modulators of δ-ENaC activity, such as capsazepine and icilin (activators) and Evans blue (inhibitor), did not show effects on short-circuit current (I SC), suggesting that δ-ENaC is not involved in the modulation of transcellular sodium absorption in NCI-H441 cell monolayers. Over-expression of δ-ENaC in NCI-H441 cells resulted in detectable protein expression in the apical cell membrane, as well as capsazepine and icilin-stimulated increases in I SC that were effectively blocked by Evans blue and that were consistent with δ-ENaC activation and inhibition, respectively. Consequently, these observations suggest that δ-ENaC expression is low in NCI-H441, Calu-3, and ATI cells and does not contribute to transepithelial sodium absorption.en
dc.language.isoenen
dc.titleExpression and function of the epithelial sodium channel δ-subunit in human respiratory epithelial cells in vitro.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Institute for Pharmaceutical Research Saarland, 66123 Saarbrücken, Germanyen
dc.identifier.journalPflügers Archiv : European journal of physiologyen

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