Efficient production of soluble recombinant single chain Fv fragments by a Pseudomonas putida strain KT2440 cell factory.
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Authors
Dammeyer, ThorbenSteinwand, Miriam
Krüger, Sarah-C
Dübel, Stefan
Hust, Michael
Timmis, Kenneth N
Issue Date
2011
Metadata
Show full item recordAbstract
Recombinant antibody fragments have a wide range of applications in research, diagnostics and therapy. For many of these, small fragments like single chain fragment variables (scFv) function well and can be produced inexpensively in bacterial expression systems. Although Escherichia coli K-12 production systems are convenient, yields of different fragments, even those produced from codon-optimized expression systems, vary significantly. Where yields are inadequate, alternative production systems are needed. Pseudomonas putida strain KT2440 is a versatile biosafety strain known for good expression of heterologous genes, so we have explored its utility as a cell factory for production of scFvs.Citation
Efficient production of soluble recombinant single chain Fv fragments by a Pseudomonas putida strain KT2440 cell factory. 2011, 10:11 Microb. Cell Fact.Affiliation
Helmholtz Centre for infection research, Inhoffenstr. 7, D-38124 Braunschweig, Germany.Journal
Microbial cell factoriesPubMed ID
21338491Type
ArticleLanguage
enISSN
1475-2859ae974a485f413a2113503eed53cd6c53
10.1186/1475-2859-10-11
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