Generation of anti-TLR2 intrabody mediating inhibition of macrophage surface TLR2 expression and TLR2-driven cell activation.

2.50
Hdl Handle:
http://hdl.handle.net/10033/594417
Title:
Generation of anti-TLR2 intrabody mediating inhibition of macrophage surface TLR2 expression and TLR2-driven cell activation.
Authors:
Kirschning, Carsten J; Dreher, Stefan; Maass, Björn; Fichte, Sylvia; Schade, Jutta; Köster, Mario; Noack, Andreas; Lindenmaier, Werner; Wagner, Hermann; Böldicke, Thomas
Abstract:
Toll-like receptor (TLR) 2 is a component of the innate immune system and senses specific pathogen associated molecular patterns (PAMPs) of both microbial and viral origin. Cell activation via TLR2 and other pattern recognition receptors (PRRs) contributes to sepsis pathology and chronic inflammation both relying on overamplification of an immune response. Intracellular antibodies expressed and retained inside the endoplasmatic reticulum (ER-intrabodies) are applied to block translocation of secreted and cell surface molecules from the ER to the cell surface resulting in functional inhibition of the target protein. Here we describe generation and application of a functional anti-TLR2 ER intrabody (alphaT2ib) which was generated from an antagonistic monoclonal antibody (mAb) towards human and murine TLR2 (T2.5) to inhibit the function of TLR2. alphaT2ib is a scFv fragment comprising the variable domain of the heavy chain and the variable domain of the light chain of mAb T2.5 linked together by a synthetic (Gly4Ser)3 amino acid sequence.
Affiliation:
Helmholtz Centre for infection research, Inhoffenstr. 7, D-38124 Braunschweig, Germany.
Citation:
Generation of anti-TLR2 intrabody mediating inhibition of macrophage surface TLR2 expression and TLR2-driven cell activation. 2010, 10:31 BMC Biotechnol.
Journal:
BMC biotechnology
Issue Date:
2010
URI:
http://hdl.handle.net/10033/594417
DOI:
10.1186/1472-6750-10-31
PubMed ID:
20388199
Type:
Article
Language:
en
ISSN:
1472-6750
Appears in Collections:
publications of the research group recombinant protein expression (RPEX)

Full metadata record

DC FieldValue Language
dc.contributor.authorKirschning, Carsten Jen
dc.contributor.authorDreher, Stefanen
dc.contributor.authorMaass, Björnen
dc.contributor.authorFichte, Sylviaen
dc.contributor.authorSchade, Juttaen
dc.contributor.authorKöster, Marioen
dc.contributor.authorNoack, Andreasen
dc.contributor.authorLindenmaier, Werneren
dc.contributor.authorWagner, Hermannen
dc.contributor.authorBöldicke, Thomasen
dc.date.accessioned2016-01-20T14:17:07Zen
dc.date.available2016-01-20T14:17:07Zen
dc.date.issued2010en
dc.identifier.citationGeneration of anti-TLR2 intrabody mediating inhibition of macrophage surface TLR2 expression and TLR2-driven cell activation. 2010, 10:31 BMC Biotechnol.en
dc.identifier.issn1472-6750en
dc.identifier.pmid20388199en
dc.identifier.doi10.1186/1472-6750-10-31en
dc.identifier.urihttp://hdl.handle.net/10033/594417en
dc.description.abstractToll-like receptor (TLR) 2 is a component of the innate immune system and senses specific pathogen associated molecular patterns (PAMPs) of both microbial and viral origin. Cell activation via TLR2 and other pattern recognition receptors (PRRs) contributes to sepsis pathology and chronic inflammation both relying on overamplification of an immune response. Intracellular antibodies expressed and retained inside the endoplasmatic reticulum (ER-intrabodies) are applied to block translocation of secreted and cell surface molecules from the ER to the cell surface resulting in functional inhibition of the target protein. Here we describe generation and application of a functional anti-TLR2 ER intrabody (alphaT2ib) which was generated from an antagonistic monoclonal antibody (mAb) towards human and murine TLR2 (T2.5) to inhibit the function of TLR2. alphaT2ib is a scFv fragment comprising the variable domain of the heavy chain and the variable domain of the light chain of mAb T2.5 linked together by a synthetic (Gly4Ser)3 amino acid sequence.en
dc.language.isoenen
dc.subject.meshAdenoviridaeen
dc.subject.meshAmino Acid Sequenceen
dc.subject.meshAnimalsen
dc.subject.meshAntibodies, Monoclonalen
dc.subject.meshAntibody Specificityen
dc.subject.meshBase Sequenceen
dc.subject.meshCell Lineen
dc.subject.meshEndoplasmic Reticulumen
dc.subject.meshGenetic Vectorsen
dc.subject.meshHumansen
dc.subject.meshInterleukin-6en
dc.subject.meshMacrophagesen
dc.subject.meshMiceen
dc.subject.meshMice, Inbred C57BLen
dc.subject.meshMolecular Sequence Dataen
dc.subject.meshSignal Transductionen
dc.subject.meshSingle-Chain Antibodiesen
dc.subject.meshToll-Like Receptor 2en
dc.subject.meshTransfectionen
dc.subject.meshTumor Necrosis Factor-alphaen
dc.titleGeneration of anti-TLR2 intrabody mediating inhibition of macrophage surface TLR2 expression and TLR2-driven cell activation.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection research, Inhoffenstr. 7, D-38124 Braunschweig, Germany.en
dc.identifier.journalBMC biotechnologyen

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