2.50
Hdl Handle:
http://hdl.handle.net/10033/601377
Title:
Interferon-γ-inducible Rab20 regulates endosomal morphology and EGFR degradation in macrophages.
Authors:
Pei, Gang; Schnettger, Laura; Bronietzki, Marc; Repnik, Urska; Griffiths, Gareth; Gutierrez, Maximiliano Gabriel
Abstract:
Little is known about the molecular players that regulate changes in the endocytic pathway during immune activation. Here we investigate the role of Rab20 in the endocytic pathway during activation of macrophages. Rab20 is associated with endocytic structures, but the function of this Rab GTPase in the endocytic pathway remains poorly characterized. We find that in macrophages, Rab20 expression and endosomal association significantly increase after interferon-γ (IFN-γ) treatment. Moreover, IFN-γ and Rab20 expression induce a dramatic enlargement of endosomes. These enlarged endosomes are the result of homotypic fusion promoted by Rab20 expression. The expression of Rab20 or the dominant-negative mutant Rab20T19N does not affect transferrin or dextran 70 kDa uptake. However, knockdown of Rab20 accelerates epidermal growth factor (EGF) trafficking to LAMP-2-positive compartments and EGF receptor degradation. Thus this work defines a function for Rab20 in the endocytic pathway during immune activation of macrophages.
Affiliation:
Helmholtz Centre for infection research (HZI), 38124 Braunschweig, Germany.
Citation:
Interferon-γ-inducible Rab20 regulates endosomal morphology and EGFR degradation in macrophages. 2015, 26 (17):3061-70 Mol. Biol. Cell
Journal:
Molecular biology of the cell
Issue Date:
1-Sep-2015
URI:
http://hdl.handle.net/10033/601377
DOI:
10.1091/mbc.E14-11-1547
PubMed ID:
26157167
Type:
Article
Language:
en
ISSN:
1939-4586
Appears in Collections:
Publications of the RG Phagosomen Biologie

Full metadata record

DC FieldValue Language
dc.contributor.authorPei, Gangen
dc.contributor.authorSchnettger, Lauraen
dc.contributor.authorBronietzki, Marcen
dc.contributor.authorRepnik, Urskaen
dc.contributor.authorGriffiths, Garethen
dc.contributor.authorGutierrez, Maximiliano Gabrielen
dc.date.accessioned2016-03-16T11:54:07Zen
dc.date.available2016-03-16T11:54:07Zen
dc.date.issued2015-09-01en
dc.identifier.citationInterferon-γ-inducible Rab20 regulates endosomal morphology and EGFR degradation in macrophages. 2015, 26 (17):3061-70 Mol. Biol. Cellen
dc.identifier.issn1939-4586en
dc.identifier.pmid26157167en
dc.identifier.doi10.1091/mbc.E14-11-1547en
dc.identifier.urihttp://hdl.handle.net/10033/601377en
dc.description.abstractLittle is known about the molecular players that regulate changes in the endocytic pathway during immune activation. Here we investigate the role of Rab20 in the endocytic pathway during activation of macrophages. Rab20 is associated with endocytic structures, but the function of this Rab GTPase in the endocytic pathway remains poorly characterized. We find that in macrophages, Rab20 expression and endosomal association significantly increase after interferon-γ (IFN-γ) treatment. Moreover, IFN-γ and Rab20 expression induce a dramatic enlargement of endosomes. These enlarged endosomes are the result of homotypic fusion promoted by Rab20 expression. The expression of Rab20 or the dominant-negative mutant Rab20T19N does not affect transferrin or dextran 70 kDa uptake. However, knockdown of Rab20 accelerates epidermal growth factor (EGF) trafficking to LAMP-2-positive compartments and EGF receptor degradation. Thus this work defines a function for Rab20 in the endocytic pathway during immune activation of macrophages.en
dc.language.isoenen
dc.titleInterferon-γ-inducible Rab20 regulates endosomal morphology and EGFR degradation in macrophages.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection research (HZI), 38124 Braunschweig, Germany.en
dc.identifier.journalMolecular biology of the cellen
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