Human alveolar epithelial cells expressing tight junctions to model the air-blood barrier.

2.50
Hdl Handle:
http://hdl.handle.net/10033/604746
Title:
Human alveolar epithelial cells expressing tight junctions to model the air-blood barrier.
Authors:
Kuehn, Anna; Kletting, Stephanie; de Souza Carvalho-Wodarz, Cristiane; Repnik, Urska; Griffiths, Gareth; Fischer, Ulrike; Meese, Eckart; Huwer, Hanno; Wirth, Dagmar; May, Tobias; Schneider-Daum, Nicole; Lehr, Claus Michael
Abstract:
This paper describes a new human alveolar epithelial cell line (hAELVi - human Alveolar Epithelial Lentivirus immortalized) with type I-like characteristics and functional tight junctions, suitable to model the air-blood barrier of the peripheral lung. Primary human alveolar epithelial cells were immortalized by a novel regimen, grown as monolayers on permeable filter supports and characterized morphologically, biochemically and biophysically. hAELVi cells maintain the capacity to form tight intercellular junctions, with high trans-epithelial electrical resistance (> 1000 Ω*cm²). The cells could be kept in culture over several days, up to passage 75, under liquid-liquid as well as air-liquid conditions. Ultrastructural analysis and real time PCR revealed type I-like cell properties, such as the presence of caveolae, expression of caveolin-1, and absence of surfactant protein C. Accounting for the barrier properties, inter-digitations sealed with tight junctions and desmosomes were also observed. Low permeability of the hydrophilic marker sodium fluorescein confirmed the suitability of hAELVi cells for in vitro transport studies across the alveolar epithelium. These results suggest that hAELVi cells reflect the essential features of the air-blood barrier, as needed for an alternative to animal testing to study absorption and toxicity of inhaled drugs, chemicals and nanomaterials.
Affiliation:
Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarland University, Campus C2.3, D-66123 Saarbrücken, Germany.
Citation:
Human alveolar epithelial cells expressing tight junctions to model the air-blood barrier. 2016: ALTEX
Journal:
ALTEX
Issue Date:
17-Mar-2016
URI:
http://hdl.handle.net/10033/604746
DOI:
10.14573/altex.1511131
PubMed ID:
26985677
Type:
Article
ISSN:
1868-596X
Appears in Collections:
publications of the department drug delivery ([TC] DDEL)

Full metadata record

DC FieldValue Language
dc.contributor.authorKuehn, Annaen
dc.contributor.authorKletting, Stephanieen
dc.contributor.authorde Souza Carvalho-Wodarz, Cristianeen
dc.contributor.authorRepnik, Urskaen
dc.contributor.authorGriffiths, Garethen
dc.contributor.authorFischer, Ulrikeen
dc.contributor.authorMeese, Eckarten
dc.contributor.authorHuwer, Hannoen
dc.contributor.authorWirth, Dagmaren
dc.contributor.authorMay, Tobiasen
dc.contributor.authorSchneider-Daum, Nicoleen
dc.contributor.authorLehr, Claus Michaelen
dc.date.accessioned2016-04-07T14:39:20Zen
dc.date.available2016-04-07T14:39:20Zen
dc.date.issued2016-03-17en
dc.identifier.citationHuman alveolar epithelial cells expressing tight junctions to model the air-blood barrier. 2016: ALTEXen
dc.identifier.issn1868-596Xen
dc.identifier.pmid26985677en
dc.identifier.doi10.14573/altex.1511131en
dc.identifier.urihttp://hdl.handle.net/10033/604746en
dc.description.abstractThis paper describes a new human alveolar epithelial cell line (hAELVi - human Alveolar Epithelial Lentivirus immortalized) with type I-like characteristics and functional tight junctions, suitable to model the air-blood barrier of the peripheral lung. Primary human alveolar epithelial cells were immortalized by a novel regimen, grown as monolayers on permeable filter supports and characterized morphologically, biochemically and biophysically. hAELVi cells maintain the capacity to form tight intercellular junctions, with high trans-epithelial electrical resistance (> 1000 Ω*cm²). The cells could be kept in culture over several days, up to passage 75, under liquid-liquid as well as air-liquid conditions. Ultrastructural analysis and real time PCR revealed type I-like cell properties, such as the presence of caveolae, expression of caveolin-1, and absence of surfactant protein C. Accounting for the barrier properties, inter-digitations sealed with tight junctions and desmosomes were also observed. Low permeability of the hydrophilic marker sodium fluorescein confirmed the suitability of hAELVi cells for in vitro transport studies across the alveolar epithelium. These results suggest that hAELVi cells reflect the essential features of the air-blood barrier, as needed for an alternative to animal testing to study absorption and toxicity of inhaled drugs, chemicals and nanomaterials.en
dc.languageENGen
dc.titleHuman alveolar epithelial cells expressing tight junctions to model the air-blood barrier.en
dc.typeArticleen
dc.contributor.departmentHelmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarland University, Campus C2.3, D-66123 Saarbrücken, Germany.en
dc.identifier.journalALTEXen

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