2.50
Hdl Handle:
http://hdl.handle.net/10033/605860
Title:
Mutations improving production and secretion of extracellular lipase by Burkholderia glumae PG1.
Authors:
Knapp, Andreas; Voget, Sonja; Gao, Rong; Zaburannyi, Nestor; Krysciak, Dagmar; Breuer, Michael; Hauer, Bernhard; Streit, Wolfgang R; Müller, Rolf ( 0000-0002-1042-5665 ) ; Daniel, Rolf; Jaeger, Karl-Erich
Abstract:
Burkholderia glumae is a Gram-negative phytopathogenic bacterium known as the causative agent of rice panicle blight. Strain B. glumae PG1 is used for the production of a biotechnologically relevant lipase, which is secreted into the culture supernatant via a type II secretion pathway. We have comparatively analyzed the genome sequences of B. glumae PG1 wild type and a lipase overproducing strain obtained by classical strain mutagenesis. Among a total number of 72 single nucleotide polymorphisms (SNPs) identified in the genome of the production strain, two were localized in front of the lipAB operon and were analyzed in detail. Both mutations contribute to a 100-fold overproduction of extracellular lipase in B. glumae PG1 by affecting transcription of the lipAB operon and efficiency of lipase secretion. We analyzed each of the two SNPs separately and observed a stronger influence of the promoter mutation than of the signal peptide modification but also a cumulative effect of both mutations. Furthermore, fusion of the mutated LipA signal peptide resulted in a 2-fold increase in secretion of the heterologous reporter alkaline phosphatase from Escherichia coli.
Affiliation:
Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS),Saarland 9 University, 66123 Saarbrücken, Germany.
Citation:
Mutations improving production and secretion of extracellular lipase by Burkholderia glumae PG1. 2015: Appl. Microbiol. Biotechnol.
Journal:
Applied microbiology and biotechnology
Issue Date:
17-Oct-2015
URI:
http://hdl.handle.net/10033/605860
DOI:
10.1007/s00253-015-7041-z
PubMed ID:
26476653
Type:
Article
ISSN:
1432-0614
Appears in Collections:
publications of the department of microbial natural substances ([HIPS]MINS)

Full metadata record

DC FieldValue Language
dc.contributor.authorKnapp, Andreasen
dc.contributor.authorVoget, Sonjaen
dc.contributor.authorGao, Rongen
dc.contributor.authorZaburannyi, Nestoren
dc.contributor.authorKrysciak, Dagmaren
dc.contributor.authorBreuer, Michaelen
dc.contributor.authorHauer, Bernharden
dc.contributor.authorStreit, Wolfgang Ren
dc.contributor.authorMüller, Rolfen
dc.contributor.authorDaniel, Rolfen
dc.contributor.authorJaeger, Karl-Erichen
dc.date.accessioned2016-04-19T09:34:05Zen
dc.date.available2016-04-19T09:34:05Zen
dc.date.issued2015-10-17en
dc.identifier.citationMutations improving production and secretion of extracellular lipase by Burkholderia glumae PG1. 2015: Appl. Microbiol. Biotechnol.en
dc.identifier.issn1432-0614en
dc.identifier.pmid26476653en
dc.identifier.doi10.1007/s00253-015-7041-zen
dc.identifier.urihttp://hdl.handle.net/10033/605860en
dc.description.abstractBurkholderia glumae is a Gram-negative phytopathogenic bacterium known as the causative agent of rice panicle blight. Strain B. glumae PG1 is used for the production of a biotechnologically relevant lipase, which is secreted into the culture supernatant via a type II secretion pathway. We have comparatively analyzed the genome sequences of B. glumae PG1 wild type and a lipase overproducing strain obtained by classical strain mutagenesis. Among a total number of 72 single nucleotide polymorphisms (SNPs) identified in the genome of the production strain, two were localized in front of the lipAB operon and were analyzed in detail. Both mutations contribute to a 100-fold overproduction of extracellular lipase in B. glumae PG1 by affecting transcription of the lipAB operon and efficiency of lipase secretion. We analyzed each of the two SNPs separately and observed a stronger influence of the promoter mutation than of the signal peptide modification but also a cumulative effect of both mutations. Furthermore, fusion of the mutated LipA signal peptide resulted in a 2-fold increase in secretion of the heterologous reporter alkaline phosphatase from Escherichia coli.en
dc.languageENGen
dc.titleMutations improving production and secretion of extracellular lipase by Burkholderia glumae PG1.en
dc.typeArticleen
dc.contributor.departmentHelmholtz-Institute for Pharmaceutical Research Saarland (HIPS),Saarland 9 University, 66123 Saarbrücken, Germany.en
dc.identifier.journalApplied microbiology and biotechnologyen

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