2.50
Hdl Handle:
http://hdl.handle.net/10033/606026
Title:
RNA sequencing uncovers antisense RNAs and novel small RNAs in Streptococcus pyogenes.
Authors:
Le Rhun, Anaïs; Beer, Yan Yan; Reimegård, Johan; Chylinski, Krzysztof; Charpentier, Emmanuelle
Abstract:
Streptococcus pyogenes is a human pathogen responsible for a wide spectrum of diseases ranging from mild to life-threatening infections. During the infectious process, the temporal and spatial expression of pathogenicity factors is tightly controlled by a complex network of protein and RNA regulators acting in response to various environmental signals. Here, we focus on the class of small RNA regulators (sRNAs) and present the first complete analysis of sRNA sequencing data in S. pyogenes. In the SF370 clinical isolate (M1 serotype), we identified 197 and 428 putative regulatory RNAs by visual inspection and bioinformatics screening of the sequencing data, respectively. Only 35 from the 197 candidates identified by visual screening were assigned a predicted function (T-boxes, ribosomal protein leaders, characterized riboswitches or sRNAs), indicating how little is known about sRNA regulation in S. pyogenes. By comparing our list of predicted sRNAs with previous S. pyogenes sRNA screens using bioinformatics or microarrays, 92 novel sRNAs were revealed, including antisense RNAs that are for the first time shown to be expressed in this pathogen. We experimentally validated the expression of 30 novel sRNAs and antisense RNAs. We show that the expression profile of 9 sRNAs including 2 predicted regulatory elements is affected by the endoribonucleases RNase III and/or RNase Y, highlighting the critical role of these enzymes in sRNA regulation.
Affiliation:
Helmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.
Citation:
RNA sequencing uncovers antisense RNAs and novel small RNAs in Streptococcus pyogenes. 2016, 13 (2):177-95 RNA Biol
Journal:
RNA biology
Issue Date:
Feb-2016
URI:
http://hdl.handle.net/10033/606026
DOI:
10.1080/15476286.2015.1110674
PubMed ID:
26580233
Type:
Article
Language:
en
ISSN:
1555-8584
Appears in Collections:
publications of the department Regulation of infection

Full metadata record

DC FieldValue Language
dc.contributor.authorLe Rhun, Anaïsen
dc.contributor.authorBeer, Yan Yanen
dc.contributor.authorReimegård, Johanen
dc.contributor.authorChylinski, Krzysztofen
dc.contributor.authorCharpentier, Emmanuelleen
dc.date.accessioned2016-04-20T08:28:53Zen
dc.date.available2016-04-20T08:28:53Zen
dc.date.issued2016-02en
dc.identifier.citationRNA sequencing uncovers antisense RNAs and novel small RNAs in Streptococcus pyogenes. 2016, 13 (2):177-95 RNA Biolen
dc.identifier.issn1555-8584en
dc.identifier.pmid26580233en
dc.identifier.doi10.1080/15476286.2015.1110674en
dc.identifier.urihttp://hdl.handle.net/10033/606026en
dc.description.abstractStreptococcus pyogenes is a human pathogen responsible for a wide spectrum of diseases ranging from mild to life-threatening infections. During the infectious process, the temporal and spatial expression of pathogenicity factors is tightly controlled by a complex network of protein and RNA regulators acting in response to various environmental signals. Here, we focus on the class of small RNA regulators (sRNAs) and present the first complete analysis of sRNA sequencing data in S. pyogenes. In the SF370 clinical isolate (M1 serotype), we identified 197 and 428 putative regulatory RNAs by visual inspection and bioinformatics screening of the sequencing data, respectively. Only 35 from the 197 candidates identified by visual screening were assigned a predicted function (T-boxes, ribosomal protein leaders, characterized riboswitches or sRNAs), indicating how little is known about sRNA regulation in S. pyogenes. By comparing our list of predicted sRNAs with previous S. pyogenes sRNA screens using bioinformatics or microarrays, 92 novel sRNAs were revealed, including antisense RNAs that are for the first time shown to be expressed in this pathogen. We experimentally validated the expression of 30 novel sRNAs and antisense RNAs. We show that the expression profile of 9 sRNAs including 2 predicted regulatory elements is affected by the endoribonucleases RNase III and/or RNase Y, highlighting the critical role of these enzymes in sRNA regulation.en
dc.language.isoenen
dc.titleRNA sequencing uncovers antisense RNAs and novel small RNAs in Streptococcus pyogenes.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.en
dc.identifier.journalRNA biologyen

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