Iron-chelating agent desferrioxamine stimulates formation of neutrophil extracellular traps (NETs) in human blood-derived neutrophils.

2.50
Hdl Handle:
http://hdl.handle.net/10033/610674
Title:
Iron-chelating agent desferrioxamine stimulates formation of neutrophil extracellular traps (NETs) in human blood-derived neutrophils.
Authors:
Völlger, Lena; Akong-Moore, Kathryn; Cox, Linda; Goldmann, Oliver; Wang, Yanming; Schäfer, Simon T; Naim, Hassan Y; Nizet, Victor; von Köckritz-Blickwede, Maren
Abstract:
Neutrophil extracellular trap (NET) formation is a significant innate immune defense mechanism against microbial infection that complements other neutrophil functions including phagocytosis and degranulation of antimicrobial peptides. NETs are decondensed chromatin structures in which antimicrobial components (histones, antimicrobial peptides and proteases) are deployed and mediate immobilization of microbes. Here we describe an effect of iron chelation on the phenotype of NET formation. Iron-chelating agent desferrioxamine (DFO) showed a modest but significant induction of NETs by freshly isolated human neutrophils as visualized and quantified by immunocytochemistry against histone-DNA complexes. Further analyses revealed that NET induction by iron chelation required NADPH-dependent production of reactive oxygen species (ROS) as well as protease and peptidyl-arginine-deiminase 4 (PAD4) activities, three key mechanistic pathways previously linked to NET formation. Our results demonstrate that iron chelation by DFO contributes to the formation of NETs and suggest a target for pharmacological manipulation of NET activity.
Affiliation:
Helmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.
Citation:
Iron-chelating agent desferrioxamine stimulates formation of neutrophil extracellular traps (NETs) in human blood-derived neutrophils. 2016, 36 (3): Biosci. Rep.
Journal:
Bioscience reports
Issue Date:
Jul-2016
URI:
http://hdl.handle.net/10033/610674
DOI:
10.1042/BSR20160031
PubMed ID:
27129288
Type:
Article
Language:
en
ISSN:
1573-4935
Appears in Collections:
publications of the research group immunology of infection (INI)

Full metadata record

DC FieldValue Language
dc.contributor.authorVöllger, Lenaen
dc.contributor.authorAkong-Moore, Kathrynen
dc.contributor.authorCox, Lindaen
dc.contributor.authorGoldmann, Oliveren
dc.contributor.authorWang, Yanmingen
dc.contributor.authorSchäfer, Simon Ten
dc.contributor.authorNaim, Hassan Yen
dc.contributor.authorNizet, Victoren
dc.contributor.authorvon Köckritz-Blickwede, Marenen
dc.date.accessioned2016-05-25T08:54:40Zen
dc.date.available2016-05-25T08:54:40Zen
dc.date.issued2016-07en
dc.identifier.citationIron-chelating agent desferrioxamine stimulates formation of neutrophil extracellular traps (NETs) in human blood-derived neutrophils. 2016, 36 (3): Biosci. Rep.en
dc.identifier.issn1573-4935en
dc.identifier.pmid27129288en
dc.identifier.doi10.1042/BSR20160031en
dc.identifier.urihttp://hdl.handle.net/10033/610674en
dc.description.abstractNeutrophil extracellular trap (NET) formation is a significant innate immune defense mechanism against microbial infection that complements other neutrophil functions including phagocytosis and degranulation of antimicrobial peptides. NETs are decondensed chromatin structures in which antimicrobial components (histones, antimicrobial peptides and proteases) are deployed and mediate immobilization of microbes. Here we describe an effect of iron chelation on the phenotype of NET formation. Iron-chelating agent desferrioxamine (DFO) showed a modest but significant induction of NETs by freshly isolated human neutrophils as visualized and quantified by immunocytochemistry against histone-DNA complexes. Further analyses revealed that NET induction by iron chelation required NADPH-dependent production of reactive oxygen species (ROS) as well as protease and peptidyl-arginine-deiminase 4 (PAD4) activities, three key mechanistic pathways previously linked to NET formation. Our results demonstrate that iron chelation by DFO contributes to the formation of NETs and suggest a target for pharmacological manipulation of NET activity.en
dc.language.isoenen
dc.titleIron-chelating agent desferrioxamine stimulates formation of neutrophil extracellular traps (NETs) in human blood-derived neutrophils.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.en
dc.identifier.journalBioscience reportsen

Related articles on PubMed

This item is licensed under a Creative Commons License
Creative Commons
All Items in HZI are protected by copyright, with all rights reserved, unless otherwise indicated.