Overproduction of Magnetosomes by Genomic Amplification of Biosynthesis-Related Gene Clusters in a Magnetotactic Bacterium.

2.50
Hdl Handle:
http://hdl.handle.net/10033/611256
Title:
Overproduction of Magnetosomes by Genomic Amplification of Biosynthesis-Related Gene Clusters in a Magnetotactic Bacterium.
Authors:
Lohße, Anna; Kolinko, Isabel; Raschdorf, Oliver; Uebe, René; Borg, Sarah; Brachmann, Andreas; Plitzko, Jürgen M; Müller, Rolf ( 0000-0002-1042-5665 ) ; Zhang, Youming; Schüler, Dirk
Abstract:
Magnetotactic bacteria biosynthesize specific organelles, the magnetosomes, which are membrane-enclosed crystals of a magnetic iron mineral that are aligned in a linear chain. The number and size of magnetosome particles have to be critically controlled to build a sensor sufficiently strong to ensure the efficient alignment of cells within Earth's weak magnetic field while at the same time minimizing the metabolic costs imposed by excessive magnetosome biosynthesis. Apart from their biological function, bacterial magnetosomes have gained considerable interest since they provide a highly useful model for prokaryotic organelle formation and represent biogenic magnetic nanoparticles with exceptional properties. However, potential applications have been hampered by the difficult cultivation of these fastidious bacteria and their poor yields of magnetosomes. In this study, we found that the size and number of magnetosomes within the cell are controlled by many different Mam and Mms proteins. We present a strategy for the overexpression of magnetosome biosynthesis genes in the alphaproteobacterium Magnetospirillum gryphiswaldense by chromosomal multiplication of individual and multiple magnetosome gene clusters via transposition. While stepwise amplification of the mms6 operon resulted in the formation of increasingly larger crystals (increase of ∼35%), the duplication of all major magnetosome operons (mamGFDC, mamAB, mms6, and mamXY, comprising 29 genes in total) yielded an overproducing strain in which magnetosome numbers were 2.2-fold increased. We demonstrate that the tuned expression of the mam and mms clusters provides a powerful strategy for the control of magnetosome size and number, thereby setting the stage for high-yield production of tailored magnetic nanoparticles by synthetic biology approaches.
Affiliation:
Helmholtz-Institut für pharmaceutische Forschung Saarland, Universitätscampus E8.1, 66123 Saarbrücken, Germany.
Citation:
Overproduction of Magnetosomes by Genomic Amplification of Biosynthesis-Related Gene Clusters in a Magnetotactic Bacterium. 2016, 82 (10):3032-41 Appl. Environ. Microbiol.
Journal:
Applied and environmental microbiology
Issue Date:
15-May-2016
URI:
http://hdl.handle.net/10033/611256
DOI:
10.1128/AEM.03860-15
PubMed ID:
26969709
Type:
Article
Language:
en
ISSN:
1098-5336
Appears in Collections:
publications of the department of microbial natural substances ([HIPS]MINS)

Full metadata record

DC FieldValue Language
dc.contributor.authorLohße, Annaen
dc.contributor.authorKolinko, Isabelen
dc.contributor.authorRaschdorf, Oliveren
dc.contributor.authorUebe, Renéen
dc.contributor.authorBorg, Sarahen
dc.contributor.authorBrachmann, Andreasen
dc.contributor.authorPlitzko, Jürgen Men
dc.contributor.authorMüller, Rolfen
dc.contributor.authorZhang, Youmingen
dc.contributor.authorSchüler, Dirken
dc.date.accessioned2016-05-31T14:11:54Zen
dc.date.available2016-05-31T14:11:54Zen
dc.date.issued2016-05-15en
dc.identifier.citationOverproduction of Magnetosomes by Genomic Amplification of Biosynthesis-Related Gene Clusters in a Magnetotactic Bacterium. 2016, 82 (10):3032-41 Appl. Environ. Microbiol.en
dc.identifier.issn1098-5336en
dc.identifier.pmid26969709en
dc.identifier.doi10.1128/AEM.03860-15en
dc.identifier.urihttp://hdl.handle.net/10033/611256en
dc.description.abstractMagnetotactic bacteria biosynthesize specific organelles, the magnetosomes, which are membrane-enclosed crystals of a magnetic iron mineral that are aligned in a linear chain. The number and size of magnetosome particles have to be critically controlled to build a sensor sufficiently strong to ensure the efficient alignment of cells within Earth's weak magnetic field while at the same time minimizing the metabolic costs imposed by excessive magnetosome biosynthesis. Apart from their biological function, bacterial magnetosomes have gained considerable interest since they provide a highly useful model for prokaryotic organelle formation and represent biogenic magnetic nanoparticles with exceptional properties. However, potential applications have been hampered by the difficult cultivation of these fastidious bacteria and their poor yields of magnetosomes. In this study, we found that the size and number of magnetosomes within the cell are controlled by many different Mam and Mms proteins. We present a strategy for the overexpression of magnetosome biosynthesis genes in the alphaproteobacterium Magnetospirillum gryphiswaldense by chromosomal multiplication of individual and multiple magnetosome gene clusters via transposition. While stepwise amplification of the mms6 operon resulted in the formation of increasingly larger crystals (increase of ∼35%), the duplication of all major magnetosome operons (mamGFDC, mamAB, mms6, and mamXY, comprising 29 genes in total) yielded an overproducing strain in which magnetosome numbers were 2.2-fold increased. We demonstrate that the tuned expression of the mam and mms clusters provides a powerful strategy for the control of magnetosome size and number, thereby setting the stage for high-yield production of tailored magnetic nanoparticles by synthetic biology approaches.en
dc.language.isoenen
dc.titleOverproduction of Magnetosomes by Genomic Amplification of Biosynthesis-Related Gene Clusters in a Magnetotactic Bacterium.en
dc.typeArticleen
dc.contributor.departmentHelmholtz-Institut für pharmaceutische Forschung Saarland, Universitätscampus E8.1, 66123 Saarbrücken, Germany.en
dc.identifier.journalApplied and environmental microbiologyen

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