Cohort Study of Airway Mycobiome in Adult Cystic Fibrosis Patients: Differences in Community Structure between Fungi and Bacteria Reveal Predominance of Transient Fungal Elements.

2.50
Hdl Handle:
http://hdl.handle.net/10033/611567
Title:
Cohort Study of Airway Mycobiome in Adult Cystic Fibrosis Patients: Differences in Community Structure between Fungi and Bacteria Reveal Predominance of Transient Fungal Elements.
Authors:
Kramer, Rolf; Sauer-Heilborn, Annette; Welte, Tobias; Guzman, Carlos A; Abraham, Wolf-Rainer ( 0000-0002-2850-2649 ) ; Höfle, Manfred G
Abstract:
The respiratory mycobiome is an important but understudied component of the human microbiota. Like bacteria, fungi can cause severe lung diseases, but their infection rates are much lower. This study compared the bacterial and fungal communities of sputum samples from a large cohort of 56 adult patients with cystic fibrosis (CF) during nonexacerbation periods and under continuous antibiotic treatment. Molecular fingerprinting based on single-strand conformation polymorphism (SSCP) analysis revealed fundamental differences between bacterial and fungal communities. Both groups of microorganisms were taxonomically classified by identification of gene sequences (16S rRNA and internal transcript spacer), and prevalences of single taxa were determined for the entire cohort. Major bacterial pathogens were frequently observed, whereas fungi of known pathogenicity in CF were detected only in low numbers. Fungal species richness increased without reaching a constant level (saturation), whereas bacterial richness showed saturation after 50 patients were analyzed. In contrast to bacteria, a large number of fungal species were observed together with high fluctuations over time and among patients. These findings demonstrated that the mycobiome was dominated by transient species, which strongly suggested that the main driving force was their presence in inhaled air rather than colonization. Considering the high exposure of human airways to fungal spores, we concluded that fungi have low colonization abilities in CF, and colonization by pathogenic fungal species may be considered a rare event. A comprehensive understanding of the conditions promoting fungal colonization may offer the opportunity to prevent colonization and substantially reduce or even eliminate fungus-related disease progression in CF.
Affiliation:
Helmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.
Citation:
Cohort Study of Airway Mycobiome in Adult Cystic Fibrosis Patients: Differences in Community Structure between Fungi and Bacteria Reveal Predominance of Transient Fungal Elements. 2015, 53 (9):2900-7 J. Clin. Microbiol.
Journal:
Journal of clinical microbiology
Issue Date:
Sep-2015
URI:
http://hdl.handle.net/10033/611567
DOI:
10.1128/JCM.01094-15
PubMed ID:
26135861
Type:
Article
Language:
en
ISSN:
1098-660X
Appears in Collections:
publications of the research group microbial diagnosis (MIDI)

Full metadata record

DC FieldValue Language
dc.contributor.authorKramer, Rolfen
dc.contributor.authorSauer-Heilborn, Annetteen
dc.contributor.authorWelte, Tobiasen
dc.contributor.authorGuzman, Carlos Aen
dc.contributor.authorAbraham, Wolf-Raineren
dc.contributor.authorHöfle, Manfred Gen
dc.date.accessioned2016-06-02T14:00:58Zen
dc.date.available2016-06-02T14:00:58Zen
dc.date.issued2015-09en
dc.identifier.citationCohort Study of Airway Mycobiome in Adult Cystic Fibrosis Patients: Differences in Community Structure between Fungi and Bacteria Reveal Predominance of Transient Fungal Elements. 2015, 53 (9):2900-7 J. Clin. Microbiol.en
dc.identifier.issn1098-660Xen
dc.identifier.pmid26135861en
dc.identifier.doi10.1128/JCM.01094-15en
dc.identifier.urihttp://hdl.handle.net/10033/611567en
dc.description.abstractThe respiratory mycobiome is an important but understudied component of the human microbiota. Like bacteria, fungi can cause severe lung diseases, but their infection rates are much lower. This study compared the bacterial and fungal communities of sputum samples from a large cohort of 56 adult patients with cystic fibrosis (CF) during nonexacerbation periods and under continuous antibiotic treatment. Molecular fingerprinting based on single-strand conformation polymorphism (SSCP) analysis revealed fundamental differences between bacterial and fungal communities. Both groups of microorganisms were taxonomically classified by identification of gene sequences (16S rRNA and internal transcript spacer), and prevalences of single taxa were determined for the entire cohort. Major bacterial pathogens were frequently observed, whereas fungi of known pathogenicity in CF were detected only in low numbers. Fungal species richness increased without reaching a constant level (saturation), whereas bacterial richness showed saturation after 50 patients were analyzed. In contrast to bacteria, a large number of fungal species were observed together with high fluctuations over time and among patients. These findings demonstrated that the mycobiome was dominated by transient species, which strongly suggested that the main driving force was their presence in inhaled air rather than colonization. Considering the high exposure of human airways to fungal spores, we concluded that fungi have low colonization abilities in CF, and colonization by pathogenic fungal species may be considered a rare event. A comprehensive understanding of the conditions promoting fungal colonization may offer the opportunity to prevent colonization and substantially reduce or even eliminate fungus-related disease progression in CF.en
dc.language.isoenen
dc.subject.meshAdolescenten
dc.subject.meshAdulten
dc.subject.meshBacteriaen
dc.subject.meshBiotaen
dc.subject.meshCohort Studiesen
dc.subject.meshCystic Fibrosisen
dc.subject.meshDNA, Bacterialen
dc.subject.meshDNA, Fungalen
dc.subject.meshDNA, Intergenicen
dc.subject.meshDNA, Ribosomalen
dc.subject.meshFemaleen
dc.subject.meshFungien
dc.subject.meshHumansen
dc.subject.meshMaleen
dc.subject.meshMiddle Ageden
dc.subject.meshMolecular Sequence Dataen
dc.subject.meshPhylogenyen
dc.subject.meshRNA, Ribosomal, 16Sen
dc.subject.meshRespiratory Tract Infectionsen
dc.subject.meshSequence Analysis, DNAen
dc.subject.meshSputumen
dc.subject.meshYoung Adulten
dc.titleCohort Study of Airway Mycobiome in Adult Cystic Fibrosis Patients: Differences in Community Structure between Fungi and Bacteria Reveal Predominance of Transient Fungal Elements.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.en
dc.identifier.journalJournal of clinical microbiologyen

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