2.50
Hdl Handle:
http://hdl.handle.net/10033/611687
Title:
Screening of Compounds against Gardnerella vaginalis Biofilms.
Authors:
Gottschick, Cornelia; Szafranski, Szymon P; Kunze, Brigitte; Sztajer, Helena; Masur, Clarissa; Abels, Christoph; Wagner-Döbler, Irene
Abstract:
Bacterial vaginosis (BV) is a common infection in reproductive age woman and is characterized by dysbiosis of the healthy vaginal flora which is dominated by Lactobacilli, followed by growth of bacteria like Gardnerella vaginalis. The ability of G. vaginalis to form biofilms contributes to the high rates of recurrence that are typical for BV and which unfortunately make repeated antibiotic therapy inevitable. Here we developed a biofilm model for G. vaginalis and screened a large spectrum of compounds for their ability to prevent biofilm formation and to resolve an existing G. vaginalis biofilm. The antibiotics metronidazole and tobramycin were highly effective in preventing biofilm formation, but had no effect on an established biofilm. The application of the amphoteric tenside sodium cocoamphoacetate (SCAA) led to disintegration of existing biofilms, reducing biomass by 51% and viability by 61% and it was able to increase the effect of metronidazole by 40% (biomass) and 61% (viability). Our data show that attacking the biofilm and the bacterial cells by the combination of an amphoteric tenside with the antibiotic metronidazole might be a useful strategy against BV.
Affiliation:
Helmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.
Citation:
Screening of Compounds against Gardnerella vaginalis Biofilms. 2016, 11 (4):e0154086 PLoS ONE
Journal:
PloS one
Issue Date:
2016
URI:
http://hdl.handle.net/10033/611687
DOI:
10.1371/journal.pone.0154086
PubMed ID:
27111438
Type:
Article
Language:
en
ISSN:
1932-6203
Appears in Collections:
collections of the research group microbial communication (KOM)

Full metadata record

DC FieldValue Language
dc.contributor.authorGottschick, Corneliaen
dc.contributor.authorSzafranski, Szymon Pen
dc.contributor.authorKunze, Brigitteen
dc.contributor.authorSztajer, Helenaen
dc.contributor.authorMasur, Clarissaen
dc.contributor.authorAbels, Christophen
dc.contributor.authorWagner-Döbler, Ireneen
dc.date.accessioned2016-06-03T10:36:52Zen
dc.date.available2016-06-03T10:36:52Zen
dc.date.issued2016en
dc.identifier.citationScreening of Compounds against Gardnerella vaginalis Biofilms. 2016, 11 (4):e0154086 PLoS ONEen
dc.identifier.issn1932-6203en
dc.identifier.pmid27111438en
dc.identifier.doi10.1371/journal.pone.0154086en
dc.identifier.urihttp://hdl.handle.net/10033/611687en
dc.description.abstractBacterial vaginosis (BV) is a common infection in reproductive age woman and is characterized by dysbiosis of the healthy vaginal flora which is dominated by Lactobacilli, followed by growth of bacteria like Gardnerella vaginalis. The ability of G. vaginalis to form biofilms contributes to the high rates of recurrence that are typical for BV and which unfortunately make repeated antibiotic therapy inevitable. Here we developed a biofilm model for G. vaginalis and screened a large spectrum of compounds for their ability to prevent biofilm formation and to resolve an existing G. vaginalis biofilm. The antibiotics metronidazole and tobramycin were highly effective in preventing biofilm formation, but had no effect on an established biofilm. The application of the amphoteric tenside sodium cocoamphoacetate (SCAA) led to disintegration of existing biofilms, reducing biomass by 51% and viability by 61% and it was able to increase the effect of metronidazole by 40% (biomass) and 61% (viability). Our data show that attacking the biofilm and the bacterial cells by the combination of an amphoteric tenside with the antibiotic metronidazole might be a useful strategy against BV.en
dc.language.isoenen
dc.titleScreening of Compounds against Gardnerella vaginalis Biofilms.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.en
dc.identifier.journalPloS oneen
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