Integrated analysis of gene expression and metabolic fluxes in PHA-producing Pseudomonas putida grown on glycerol.

2.50
Hdl Handle:
http://hdl.handle.net/10033/612003
Title:
Integrated analysis of gene expression and metabolic fluxes in PHA-producing Pseudomonas putida grown on glycerol.
Authors:
Beckers, Veronique; Poblete-Castro, Ignacio; Tomasch, Jürgen; Wittmann, Christoph
Abstract:
Given its high surplus and low cost, glycerol has emerged as interesting carbon substrate for the synthesis of value-added chemicals. The soil bacterium Pseudomonas putida KT2440 can use glycerol to synthesize medium-chain-length poly(3-hydroxyalkanoates) (mcl-PHA), a class of biopolymers of industrial interest. Here, glycerol metabolism in P. putida KT2440 was studied on the level of gene expression (transcriptome) and metabolic fluxes (fluxome), using precisely adjusted chemostat cultures, growth kinetics and stoichiometry, to gain a systematic understanding of the underlying metabolic and regulatory network.
Affiliation:
Helmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.
Citation:
Integrated analysis of gene expression and metabolic fluxes in PHA-producing Pseudomonas putida grown on glycerol. 2016, 15 (1):73 Microb. Cell Fact.
Journal:
Microbial cell factories
Issue Date:
2016
URI:
http://hdl.handle.net/10033/612003
DOI:
10.1186/s12934-016-0470-2
PubMed ID:
27142075
Type:
Article
Language:
en
ISSN:
1475-2859
Appears in Collections:
collections of the research group microbial communication (KOM)

Full metadata record

DC FieldValue Language
dc.contributor.authorBeckers, Veroniqueen
dc.contributor.authorPoblete-Castro, Ignacioen
dc.contributor.authorTomasch, Jürgenen
dc.contributor.authorWittmann, Christophen
dc.date.accessioned2016-06-07T13:47:24Zen
dc.date.available2016-06-07T13:47:24Zen
dc.date.issued2016en
dc.identifier.citationIntegrated analysis of gene expression and metabolic fluxes in PHA-producing Pseudomonas putida grown on glycerol. 2016, 15 (1):73 Microb. Cell Fact.en
dc.identifier.issn1475-2859en
dc.identifier.pmid27142075en
dc.identifier.doi10.1186/s12934-016-0470-2en
dc.identifier.urihttp://hdl.handle.net/10033/612003en
dc.description.abstractGiven its high surplus and low cost, glycerol has emerged as interesting carbon substrate for the synthesis of value-added chemicals. The soil bacterium Pseudomonas putida KT2440 can use glycerol to synthesize medium-chain-length poly(3-hydroxyalkanoates) (mcl-PHA), a class of biopolymers of industrial interest. Here, glycerol metabolism in P. putida KT2440 was studied on the level of gene expression (transcriptome) and metabolic fluxes (fluxome), using precisely adjusted chemostat cultures, growth kinetics and stoichiometry, to gain a systematic understanding of the underlying metabolic and regulatory network.en
dc.language.isoenen
dc.titleIntegrated analysis of gene expression and metabolic fluxes in PHA-producing Pseudomonas putida grown on glycerol.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.en
dc.identifier.journalMicrobial cell factoriesen

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