Echinocandin resistance and population structure of invasive Candida glabrata isolates from two university hospitals in Germany and Austria.
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Authors
Klotz, UlrikeSchmidt, Dirk
Willinger, Birgit
Steinmann, Eike
Buer, Jan
Rath, Peter-Michael
Steinmann, Joerg
Issue Date
2016-05
Metadata
Show full item recordAbstract
Echinocandin resistance in Candida glabrata is emerging and is associated with the presence of FKS mutations. In this study, we analysed the antifungal susceptibility, presence of FKS mutations and clonality of C. glabrata blood culture isolates from two hospitals in Germany and Austria. Susceptibility testing of 64 C. glabrata bloodstream isolates from two university hospitals was performed with broth microdilution method according to EUCAST. In addition, all isolates were screened for FKS mutations. Molecular fingerprinting was performed by microsatellite PCR with three separate primer pairs and semiautomated repetitive sequenced-based PCR (rep-PCR). One C. glabrata isolate from Germany (1.5%) was echinocandin resistant, with a corresponding mutation in FKS2 gene hot spot 1. The discriminatory power of microsatellite PCR was higher than that of rep-PCR (Simpson Index of 0.94 vs. 0.88); microsatellite PCR created 31 separate genotypes, whereas rep-PCR created 17. Predominant genotypes or clusters of isolates from Germany and Austria were present, with no epidemiological evidence of nosocomial transmissions. Although we found a low incidence of echinocandin resistance in C. glabrata in our settings, further surveillance projects in central Europe are warranted for monitoring future epidemiological trends. The genetic population structure of C. glabrata demonstrates overrepresented geographical clusters.Citation
Echinocandin resistance and population structure of invasive Candida glabrata isolates from two university hospitals in Germany and Austria. 2016, 59 (5):312-8 MycosesAffiliation
Twincore Centre for Experimental and Clinical Infection Research, Hannover, Germany.Journal
MycosesPubMed ID
26806376Type
ArticleLanguage
enISSN
1439-0507ae974a485f413a2113503eed53cd6c53
10.1111/myc.12472
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