2.50
Hdl Handle:
http://hdl.handle.net/10033/614799
Title:
Mixture Effects of Estrogenic Pesticides at the Human Estrogen Receptor α and β.
Authors:
Seeger, Bettina; Klawonn, Frank; Nguema Bekale, Boris; Steinberg, Pablo
Abstract:
Consumers of fruits and vegetables are frequently exposed to small amounts of hormonally active pesticides, some of them sharing a common mode of action such as the activation of the human estrogen receptor α (hERα) or β (hERβ). Therefore, it is of particular importance to evaluate risks emanating from chemical mixtures, in which the individual pesticides are present at human-relevant concentrations, below their corresponding maximum residue levels. Binary and ternary iso-effective mixtures of estrogenic pesticides at effect concentrations eliciting a 1 or 10% effect in the presence or absence of 17β-estradiol were tested experimentally at the hERα in the yeast-based estrogen screen (YES) assay as well as in the human U2-OS cell-based ERα chemical-activated luciferase gene expression (ERα CALUX) assay and at the hERβ in the ERβ CALUX assay. The outcome was then compared to predictions calculated by means of concentration addition. In most cases, additive effects were observed with the tested combinations in all three test systems, an observation that supports the need to expand the risk assessment of pesticides and consider cumulative risk assessment. An additional testing of mixture effects at the hERβ showed that most test substances being active at the hERα could also elicit additive effects at the hERβ, but the hERβ was less sensitive. In conclusion, effects of the same ligands at the hERα and the hERβ could influence the estrogenic outcome under physiological conditions.
Affiliation:
Helmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.
Citation:
Mixture Effects of Estrogenic Pesticides at the Human Estrogen Receptor α and β. 2016, 11 (1):e0147490 PLoS ONE
Journal:
PloS one
Issue Date:
2016
URI:
http://hdl.handle.net/10033/614799
DOI:
10.1371/journal.pone.0147490
PubMed ID:
26812056
Type:
Article
Language:
en
ISSN:
1932-6203
Appears in Collections:
publications of the research group Microbial Proteomics (MPRO)

Full metadata record

DC FieldValue Language
dc.contributor.authorSeeger, Bettinaen
dc.contributor.authorKlawonn, Franken
dc.contributor.authorNguema Bekale, Borisen
dc.contributor.authorSteinberg, Pabloen
dc.date.accessioned2016-06-27T09:32:08Z-
dc.date.available2016-06-27T09:32:08Z-
dc.date.issued2016-
dc.identifier.citationMixture Effects of Estrogenic Pesticides at the Human Estrogen Receptor α and β. 2016, 11 (1):e0147490 PLoS ONEen
dc.identifier.issn1932-6203-
dc.identifier.pmid26812056-
dc.identifier.doi10.1371/journal.pone.0147490-
dc.identifier.urihttp://hdl.handle.net/10033/614799-
dc.description.abstractConsumers of fruits and vegetables are frequently exposed to small amounts of hormonally active pesticides, some of them sharing a common mode of action such as the activation of the human estrogen receptor α (hERα) or β (hERβ). Therefore, it is of particular importance to evaluate risks emanating from chemical mixtures, in which the individual pesticides are present at human-relevant concentrations, below their corresponding maximum residue levels. Binary and ternary iso-effective mixtures of estrogenic pesticides at effect concentrations eliciting a 1 or 10% effect in the presence or absence of 17β-estradiol were tested experimentally at the hERα in the yeast-based estrogen screen (YES) assay as well as in the human U2-OS cell-based ERα chemical-activated luciferase gene expression (ERα CALUX) assay and at the hERβ in the ERβ CALUX assay. The outcome was then compared to predictions calculated by means of concentration addition. In most cases, additive effects were observed with the tested combinations in all three test systems, an observation that supports the need to expand the risk assessment of pesticides and consider cumulative risk assessment. An additional testing of mixture effects at the hERβ showed that most test substances being active at the hERα could also elicit additive effects at the hERβ, but the hERβ was less sensitive. In conclusion, effects of the same ligands at the hERα and the hERβ could influence the estrogenic outcome under physiological conditions.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleMixture Effects of Estrogenic Pesticides at the Human Estrogen Receptor α and β.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.en
dc.identifier.journalPloS oneen

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