2.50
Hdl Handle:
http://hdl.handle.net/10033/615497
Title:
Temperature-responsive in vitro RNA structurome of Yersinia pseudotuberculosis.
Authors:
Righetti, Francesco; Nuss, Aaron M; Twittenhoff, Christian; Beele, Sascha; Urban, Kristina; Will, Sebastian; Bernhart, Stephan H; Stadler, Peter F; Dersch, Petra ( 0000-0001-8177-3280 ) ; Narberhaus, Franz
Abstract:
RNA structures are fundamentally important for RNA function. Dynamic, condition-dependent structural changes are able to modulate gene expression as shown for riboswitches and RNA thermometers. By parallel analysis of RNA structures, we mapped the RNA structurome of Yersinia pseudotuberculosis at three different temperatures. This human pathogen is exquisitely responsive to host body temperature (37 °C), which induces a major metabolic transition. Our analysis profiles the structure of more than 1,750 RNAs at 25 °C, 37 °C, and 42 °C. Average mRNAs tend to be unstructured around the ribosome binding site. We searched for 5'-UTRs that are folded at low temperature and identified novel thermoresponsive RNA structures from diverse gene categories. The regulatory potential of 16 candidates was validated. In summary, we present a dynamic bacterial RNA structurome and find that the expression of virulence-relevant functions in Y. pseudotuberculosis and reprogramming of its metabolism in response to temperature is associated with a restructuring of numerous mRNAs.
Affiliation:
Helmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.
Citation:
Temperature-responsive in vitro RNA structurome of Yersinia pseudotuberculosis. 2016, 113 (26):7237-42 Proc. Natl. Acad. Sci. U.S.A.
Journal:
Proceedings of the National Academy of Sciences of the United States of America
Issue Date:
28-Jun-2016
URI:
http://hdl.handle.net/10033/615497
DOI:
10.1073/pnas.1523004113
PubMed ID:
27298343
Type:
Article
Language:
en
ISSN:
1091-6490
Appears in Collections:
publications of the department of molecular Infectionbiology (MIBI)

Full metadata record

DC FieldValue Language
dc.contributor.authorRighetti, Francescoen
dc.contributor.authorNuss, Aaron Men
dc.contributor.authorTwittenhoff, Christianen
dc.contributor.authorBeele, Saschaen
dc.contributor.authorUrban, Kristinaen
dc.contributor.authorWill, Sebastianen
dc.contributor.authorBernhart, Stephan Hen
dc.contributor.authorStadler, Peter Fen
dc.contributor.authorDersch, Petraen
dc.contributor.authorNarberhaus, Franzen
dc.date.accessioned2016-07-05T09:05:34Z-
dc.date.available2016-07-05T09:05:34Z-
dc.date.issued2016-06-28-
dc.identifier.citationTemperature-responsive in vitro RNA structurome of Yersinia pseudotuberculosis. 2016, 113 (26):7237-42 Proc. Natl. Acad. Sci. U.S.A.en
dc.identifier.issn1091-6490-
dc.identifier.pmid27298343-
dc.identifier.doi10.1073/pnas.1523004113-
dc.identifier.urihttp://hdl.handle.net/10033/615497-
dc.description.abstractRNA structures are fundamentally important for RNA function. Dynamic, condition-dependent structural changes are able to modulate gene expression as shown for riboswitches and RNA thermometers. By parallel analysis of RNA structures, we mapped the RNA structurome of Yersinia pseudotuberculosis at three different temperatures. This human pathogen is exquisitely responsive to host body temperature (37 °C), which induces a major metabolic transition. Our analysis profiles the structure of more than 1,750 RNAs at 25 °C, 37 °C, and 42 °C. Average mRNAs tend to be unstructured around the ribosome binding site. We searched for 5'-UTRs that are folded at low temperature and identified novel thermoresponsive RNA structures from diverse gene categories. The regulatory potential of 16 candidates was validated. In summary, we present a dynamic bacterial RNA structurome and find that the expression of virulence-relevant functions in Y. pseudotuberculosis and reprogramming of its metabolism in response to temperature is associated with a restructuring of numerous mRNAs.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleTemperature-responsive in vitro RNA structurome of Yersinia pseudotuberculosis.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.en
dc.identifier.journalProceedings of the National Academy of Sciences of the United States of Americaen

Related articles on PubMed

This item is licensed under a Creative Commons License
Creative Commons
All Items in HZI are protected by copyright, with all rights reserved, unless otherwise indicated.