Characterization of Novel Factors Involved in Swimming and Swarming Motility in Salmonella enterica Serovar Typhimurium.

2.50
Hdl Handle:
http://hdl.handle.net/10033/615603
Title:
Characterization of Novel Factors Involved in Swimming and Swarming Motility in Salmonella enterica Serovar Typhimurium.
Authors:
Deditius, Julia Andrea; Felgner, Sebastian; Spöring, Imke; Kühne, Caroline; Frahm, Michael; Rohde, Manfred ( 0000-0003-0522-3580 ) ; Weiß, Siegfried ( 0000-0002-5276-8658 ) ; Erhardt, Marc
Abstract:
Salmonella enterica utilizes flagellar motility to swim through liquid environments and on surfaces. The biosynthesis of the flagellum is regulated on various levels, including transcriptional and posttranscriptional mechanisms. Here, we investigated the motility phenotype of 24 selected single gene deletions that were previously described to display swimming and swarming motility effects. Mutations in flgE, fliH, ydiV, rfaG, yjcC, STM1267 and STM3363 showed an altered motility phenotype. Deletions of flgE and fliH displayed a non-motile phenotype in both swimming and swarming motility assays as expected. The deletions of STM1267, STM3363, ydiV, rfaG and yjcC were further analyzed in detail for flagellar and fimbrial gene expression and filament formation. A ΔydiV mutant showed increased swimming motility, but a decrease in swarming motility, which coincided with derepression of curli fimbriae. A deletion of yjcC, encoding for an EAL domain-containing protein, increased swimming motility independent on flagellar gene expression. A ΔSTM1267 mutant displayed a hypermotile phenotype on swarm agar plates and was found to have increased numbers of flagella. In contrast, a knockout of STM3363 did also display an increase in swarming motility, but did not alter flagella numbers. Finally, a deletion of the LPS biosynthesis-related protein RfaG reduced swimming and swarming motility, associated with a decrease in transcription from flagellar class II and class III promoters and a lack of flagellar filaments.
Affiliation:
Helmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.
Citation:
Characterization of Novel Factors Involved in Swimming and Swarming Motility in Salmonella enterica Serovar Typhimurium. 2015, 10 (8):e0135351 PLoS ONE
Journal:
PloS one
Issue Date:
2015
URI:
http://hdl.handle.net/10033/615603
DOI:
10.1371/journal.pone.0135351
PubMed ID:
26267246
Type:
Article
Language:
en
ISSN:
1932-6203
Appears in Collections:
publications of the junior research group infection biology of Salmonella (IBIS)

Full metadata record

DC FieldValue Language
dc.contributor.authorDeditius, Julia Andreaen
dc.contributor.authorFelgner, Sebastianen
dc.contributor.authorSpöring, Imkeen
dc.contributor.authorKühne, Carolineen
dc.contributor.authorFrahm, Michaelen
dc.contributor.authorRohde, Manfreden
dc.contributor.authorWeiß, Siegfrieden
dc.contributor.authorErhardt, Marcen
dc.date.accessioned2016-07-06T08:57:33Z-
dc.date.available2016-07-06T08:57:33Z-
dc.date.issued2015-
dc.identifier.citationCharacterization of Novel Factors Involved in Swimming and Swarming Motility in Salmonella enterica Serovar Typhimurium. 2015, 10 (8):e0135351 PLoS ONEen
dc.identifier.issn1932-6203-
dc.identifier.pmid26267246-
dc.identifier.doi10.1371/journal.pone.0135351-
dc.identifier.urihttp://hdl.handle.net/10033/615603-
dc.description.abstractSalmonella enterica utilizes flagellar motility to swim through liquid environments and on surfaces. The biosynthesis of the flagellum is regulated on various levels, including transcriptional and posttranscriptional mechanisms. Here, we investigated the motility phenotype of 24 selected single gene deletions that were previously described to display swimming and swarming motility effects. Mutations in flgE, fliH, ydiV, rfaG, yjcC, STM1267 and STM3363 showed an altered motility phenotype. Deletions of flgE and fliH displayed a non-motile phenotype in both swimming and swarming motility assays as expected. The deletions of STM1267, STM3363, ydiV, rfaG and yjcC were further analyzed in detail for flagellar and fimbrial gene expression and filament formation. A ΔydiV mutant showed increased swimming motility, but a decrease in swarming motility, which coincided with derepression of curli fimbriae. A deletion of yjcC, encoding for an EAL domain-containing protein, increased swimming motility independent on flagellar gene expression. A ΔSTM1267 mutant displayed a hypermotile phenotype on swarm agar plates and was found to have increased numbers of flagella. In contrast, a knockout of STM3363 did also display an increase in swarming motility, but did not alter flagella numbers. Finally, a deletion of the LPS biosynthesis-related protein RfaG reduced swimming and swarming motility, associated with a decrease in transcription from flagellar class II and class III promoters and a lack of flagellar filaments.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subject.meshBacterial Proteinsen
dc.subject.meshFlagellaen
dc.subject.meshGene Deletionen
dc.subject.meshMovementen
dc.subject.meshSalmonella typhimuriumen
dc.titleCharacterization of Novel Factors Involved in Swimming and Swarming Motility in Salmonella enterica Serovar Typhimurium.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.en
dc.identifier.journalPloS oneen
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