High level transient production of recombinant antibodies and antibody fusion proteins in HEK293 cells.

2.50
Hdl Handle:
http://hdl.handle.net/10033/615999
Title:
High level transient production of recombinant antibodies and antibody fusion proteins in HEK293 cells.
Authors:
Jäger, Volker; Büssow, Konrad; Wagner, Andreas; Weber, Susanne; Hust, Michael; Frenzel, André; Schirrmann, Thomas
Abstract:
The demand of monospecific high affinity binding reagents, particularly monoclonal antibodies, has been steadily increasing over the last years. Enhanced throughput of antibody generation has been addressed by optimizing in vitro selection using phage display which moved the major bottleneck to the production and purification of recombinant antibodies in an end-user friendly format. Single chain (sc)Fv antibody fragments require additional tags for detection and are not as suitable as immunoglobulins (Ig)G in many immunoassays. In contrast, the bivalent scFv-Fc antibody format shares many properties with IgG and has a very high application compatibility.
Affiliation:
Helmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.
Citation:
High level transient production of recombinant antibodies and antibody fusion proteins in HEK293 cells. 2013, 13:52 BMC Biotechnol.
Journal:
BMC biotechnology
Issue Date:
2013
URI:
http://hdl.handle.net/10033/615999
DOI:
10.1186/1472-6750-13-52
PubMed ID:
23802841
Type:
Article
Language:
en
ISSN:
1472-6750
Appears in Collections:
publications of the research group recombinant protein expression (RPEX)

Full metadata record

DC FieldValue Language
dc.contributor.authorJäger, Volkeren
dc.contributor.authorBüssow, Konraden
dc.contributor.authorWagner, Andreasen
dc.contributor.authorWeber, Susanneen
dc.contributor.authorHust, Michaelen
dc.contributor.authorFrenzel, Andréen
dc.contributor.authorSchirrmann, Thomasen
dc.date.accessioned2016-07-13T13:14:38Z-
dc.date.available2016-07-13T13:14:38Z-
dc.date.issued2013-
dc.identifier.citationHigh level transient production of recombinant antibodies and antibody fusion proteins in HEK293 cells. 2013, 13:52 BMC Biotechnol.en
dc.identifier.issn1472-6750-
dc.identifier.pmid23802841-
dc.identifier.doi10.1186/1472-6750-13-52-
dc.identifier.urihttp://hdl.handle.net/10033/615999-
dc.description.abstractThe demand of monospecific high affinity binding reagents, particularly monoclonal antibodies, has been steadily increasing over the last years. Enhanced throughput of antibody generation has been addressed by optimizing in vitro selection using phage display which moved the major bottleneck to the production and purification of recombinant antibodies in an end-user friendly format. Single chain (sc)Fv antibody fragments require additional tags for detection and are not as suitable as immunoglobulins (Ig)G in many immunoassays. In contrast, the bivalent scFv-Fc antibody format shares many properties with IgG and has a very high application compatibility.en
dc.language.isoenen
dc.subject.meshCloning, Molecularen
dc.subject.meshGenetic Vectorsen
dc.subject.meshHEK293 Cellsen
dc.subject.meshHumansen
dc.subject.meshImmunoglobulin Fc Fragmentsen
dc.subject.meshPeptide Libraryen
dc.subject.meshRecombinant Fusion Proteinsen
dc.subject.meshRibonucleasesen
dc.subject.meshSingle-Chain Antibodiesen
dc.titleHigh level transient production of recombinant antibodies and antibody fusion proteins in HEK293 cells.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.en
dc.identifier.journalBMC biotechnologyen

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