2.50
Hdl Handle:
http://hdl.handle.net/10033/616926
Title:
Topical imiquimod yields systemic effects due to unintended oral uptake.
Authors:
Grine, Lynda; Steeland, Sophie; Van Ryckeghem, Sara; Ballegeer, Marlies; Lienenklaus, Stefan; Weiss, Siegfried; Sanders, Niek N; Vandenbroucke, Roosmarijn E; Libert, Claude
Abstract:
Repetitive application of topical imiquimod is used as an experimental model for the induction of psoriasiform skin lesions in mice. The model is characterized by several inflammatory processes, including cytokine production both locally and systemically, cellular infiltration, and splenomegaly. To investigate the production of type I interferons in response to imiquimod-containing Aldara cream, IFNβ-luciferase reporter mice were imaged in vivo and ex vivo. Type I interferons were found to be produced in the skin, but also in the intestinal system caused by unintended ingestion of imiquimod by the mice. Through the use of Elizabethan collars to prevent ingestion, these effects, including psoriasiform lesions were nearly completely prevented. Our findings reveal that topical treatment with Aldara induces a psoriasiform skin inflammation, but that its mode of action depends on ingestion of the chemical, which leads to systemic responses and affects local inflammation. Therefore, potential ingestion of topical treatments during experimental procedures should be taken into account during assessment of cutaneous inflammatory parameters in skin disease models.
Affiliation:
Helmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.
Citation:
Topical imiquimod yields systemic effects due to unintended oral uptake. 2016, 6:20134 Sci Rep
Journal:
Scientific reports
Issue Date:
2016
URI:
http://hdl.handle.net/10033/616926
DOI:
10.1038/srep20134
PubMed ID:
26818707
Type:
Article
Language:
en
ISSN:
2045-2322
Appears in Collections:
publications of the research group molecular Immunology (MOLI)

Full metadata record

DC FieldValue Language
dc.contributor.authorGrine, Lyndaen
dc.contributor.authorSteeland, Sophieen
dc.contributor.authorVan Ryckeghem, Saraen
dc.contributor.authorBallegeer, Marliesen
dc.contributor.authorLienenklaus, Stefanen
dc.contributor.authorWeiss, Siegfrieden
dc.contributor.authorSanders, Niek Nen
dc.contributor.authorVandenbroucke, Roosmarijn Een
dc.contributor.authorLibert, Claudeen
dc.date.accessioned2016-07-14T08:42:41Z-
dc.date.available2016-07-14T08:42:41Z-
dc.date.issued2016-
dc.identifier.citationTopical imiquimod yields systemic effects due to unintended oral uptake. 2016, 6:20134 Sci Repen
dc.identifier.issn2045-2322-
dc.identifier.pmid26818707-
dc.identifier.doi10.1038/srep20134-
dc.identifier.urihttp://hdl.handle.net/10033/616926-
dc.description.abstractRepetitive application of topical imiquimod is used as an experimental model for the induction of psoriasiform skin lesions in mice. The model is characterized by several inflammatory processes, including cytokine production both locally and systemically, cellular infiltration, and splenomegaly. To investigate the production of type I interferons in response to imiquimod-containing Aldara cream, IFNβ-luciferase reporter mice were imaged in vivo and ex vivo. Type I interferons were found to be produced in the skin, but also in the intestinal system caused by unintended ingestion of imiquimod by the mice. Through the use of Elizabethan collars to prevent ingestion, these effects, including psoriasiform lesions were nearly completely prevented. Our findings reveal that topical treatment with Aldara induces a psoriasiform skin inflammation, but that its mode of action depends on ingestion of the chemical, which leads to systemic responses and affects local inflammation. Therefore, potential ingestion of topical treatments during experimental procedures should be taken into account during assessment of cutaneous inflammatory parameters in skin disease models.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleTopical imiquimod yields systemic effects due to unintended oral uptake.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.en
dc.identifier.journalScientific reportsen

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