2.50
Hdl Handle:
http://hdl.handle.net/10033/617266
Title:
Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
Authors:
Zühlke, Daniela; Dörries, Kirsten; Bernhardt, Jörg; Maaß, Sandra; Muntel, Jan; Liebscher, Volkmar; Pané-Farré, Jan; Riedel, Katharina; Lalk, Michael; Völker, Uwe; Engelmann, Susanne; Becher, Dörte; Fuchs, Stephan; Hecker, Michael
Abstract:
Absolute protein quantification was applied to follow the dynamics of the cytoplasmic proteome of Staphylococcus aureus in response to long-term oxygen starvation. For 1,168 proteins, the majority of all expressed proteins, molecule numbers per cell have been determined to monitor the cellular investments in single branches of bacterial life for the first time. In the presence of glucose the anaerobic protein pattern is characterized by increased amounts of glycolytic and fermentative enzymes such as Eno, GapA1, Ldh1, and PflB. Interestingly, the ferritin-like protein FtnA belongs to the most abundant proteins during anaerobic growth. Depletion of glucose finally leads to an accumulation of different enzymes such as ArcB1, ArcB2, and ArcC2 involved in arginine deiminase pathway. Concentrations of 29 exo- and 78 endometabolites were comparatively assessed and have been integrated to the metabolic networks. Here we provide an almost complete picture on the response to oxygen starvation, from signal transduction pathways to gene expression pattern, from metabolic reorganization after oxygen depletion to beginning cell death and lysis after glucose exhaustion. This experimental approach can be considered as a proof of principle how to combine cell physiology with quantitative proteomics for a new dimension in understanding simple life processes as an entity.
Affiliation:
Institute of Microbiology, Ernst-Moritz-Arndt-University Greifswald, F.-L.-Jahn-Strasse 15, D-17487 Greifswald, Germany.
Citation:
Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis. 2016, 6:28172 Sci Rep
Journal:
Scientific reports
Issue Date:
2016
URI:
http://hdl.handle.net/10033/617266
DOI:
10.1038/srep28172
PubMed ID:
27344979
Type:
Article
Language:
en
ISSN:
2045-2322
Appears in Collections:
publications of the research group Microbial Proteomics (MPRO)

Full metadata record

DC FieldValue Language
dc.contributor.authorZühlke, Danielaen
dc.contributor.authorDörries, Kirstenen
dc.contributor.authorBernhardt, Jörgen
dc.contributor.authorMaaß, Sandraen
dc.contributor.authorMuntel, Janen
dc.contributor.authorLiebscher, Volkmaren
dc.contributor.authorPané-Farré, Janen
dc.contributor.authorRiedel, Katharinaen
dc.contributor.authorLalk, Michaelen
dc.contributor.authorVölker, Uween
dc.contributor.authorEngelmann, Susanneen
dc.contributor.authorBecher, Dörteen
dc.contributor.authorFuchs, Stephanen
dc.contributor.authorHecker, Michaelen
dc.date.accessioned2016-07-20T14:43:18Z-
dc.date.available2016-07-20T14:43:18Z-
dc.date.issued2016-
dc.identifier.citationCosts of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis. 2016, 6:28172 Sci Repen
dc.identifier.issn2045-2322-
dc.identifier.pmid27344979-
dc.identifier.doi10.1038/srep28172-
dc.identifier.urihttp://hdl.handle.net/10033/617266-
dc.description.abstractAbsolute protein quantification was applied to follow the dynamics of the cytoplasmic proteome of Staphylococcus aureus in response to long-term oxygen starvation. For 1,168 proteins, the majority of all expressed proteins, molecule numbers per cell have been determined to monitor the cellular investments in single branches of bacterial life for the first time. In the presence of glucose the anaerobic protein pattern is characterized by increased amounts of glycolytic and fermentative enzymes such as Eno, GapA1, Ldh1, and PflB. Interestingly, the ferritin-like protein FtnA belongs to the most abundant proteins during anaerobic growth. Depletion of glucose finally leads to an accumulation of different enzymes such as ArcB1, ArcB2, and ArcC2 involved in arginine deiminase pathway. Concentrations of 29 exo- and 78 endometabolites were comparatively assessed and have been integrated to the metabolic networks. Here we provide an almost complete picture on the response to oxygen starvation, from signal transduction pathways to gene expression pattern, from metabolic reorganization after oxygen depletion to beginning cell death and lysis after glucose exhaustion. This experimental approach can be considered as a proof of principle how to combine cell physiology with quantitative proteomics for a new dimension in understanding simple life processes as an entity.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleCosts of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.en
dc.typeArticleen
dc.contributor.departmentInstitute of Microbiology, Ernst-Moritz-Arndt-University Greifswald, F.-L.-Jahn-Strasse 15, D-17487 Greifswald, Germany.en
dc.identifier.journalScientific reportsen

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