Dual control system - A novel scaffolding architecture of an inducible regulatory device for the precise regulation of gene expression.

2.50
Hdl Handle:
http://hdl.handle.net/10033/618499
Title:
Dual control system - A novel scaffolding architecture of an inducible regulatory device for the precise regulation of gene expression.
Authors:
Horbal, L; Luzhetskyy, A ( 0000-0001-6497-0047 )
Abstract:
Here, we present a novel scaffolding architecture of an inducible regulatory device. This dual control system is completely silent in the off stage and is coupled to the regulation of gene expression at both the transcriptional and translational levels. This system also functions as an AND gate. We demonstrated the effectiveness of the cumate-riboswitch dual control system for the control of pamamycin production in Streptomyces albus. Placing the cre recombinase gene under the control of this system permitted the construction of synthetic devices with non-volatile memory that sense the signal and respond by altering DNA at the chromosomal level, thereby producing changes that are heritable. In addition, we present a library of synthetic inducible promoters based on the previously described cumate switch. With only one inducer and different promoters, we demonstrate that simultaneous modulation of the expression of several genes to different levels in various operons is possible. Because all modules of the AND gates are functional in bacteria other than Streptomyces, we anticipate that these regulatory devices can be used to control gene expression in other Actinobacteria. The features described in this study make these systems promising tools for metabolic engineering and biotechnology in Actinobacteria.
Affiliation:
Helmholtz Institut für Pharmazeutische Forschung Saarland, Universitätscampus E8.1, 66123 Saarbrücken, Germany.
Citation:
Dual control system - A novel scaffolding architecture of an inducible regulatory device for the precise regulation of gene expression. 2016, 37:11-23 Metab. Eng.
Journal:
Metabolic engineering
Issue Date:
Sep-2016
URI:
http://hdl.handle.net/10033/618499
DOI:
10.1016/j.ymben.2016.03.008
PubMed ID:
27040671
Type:
Article
Language:
en
ISSN:
1096-7184
Appears in Collections:
publications of the research group metabolic engineering of Actinomycetes ([HIPS]AMEG)

Full metadata record

DC FieldValue Language
dc.contributor.authorHorbal, Len
dc.contributor.authorLuzhetskyy, Aen
dc.date.accessioned2016-08-17T14:06:58Z-
dc.date.available2016-08-17T14:06:58Z-
dc.date.issued2016-09-
dc.identifier.citationDual control system - A novel scaffolding architecture of an inducible regulatory device for the precise regulation of gene expression. 2016, 37:11-23 Metab. Eng.en
dc.identifier.issn1096-7184-
dc.identifier.pmid27040671-
dc.identifier.doi10.1016/j.ymben.2016.03.008-
dc.identifier.urihttp://hdl.handle.net/10033/618499-
dc.description.abstractHere, we present a novel scaffolding architecture of an inducible regulatory device. This dual control system is completely silent in the off stage and is coupled to the regulation of gene expression at both the transcriptional and translational levels. This system also functions as an AND gate. We demonstrated the effectiveness of the cumate-riboswitch dual control system for the control of pamamycin production in Streptomyces albus. Placing the cre recombinase gene under the control of this system permitted the construction of synthetic devices with non-volatile memory that sense the signal and respond by altering DNA at the chromosomal level, thereby producing changes that are heritable. In addition, we present a library of synthetic inducible promoters based on the previously described cumate switch. With only one inducer and different promoters, we demonstrate that simultaneous modulation of the expression of several genes to different levels in various operons is possible. Because all modules of the AND gates are functional in bacteria other than Streptomyces, we anticipate that these regulatory devices can be used to control gene expression in other Actinobacteria. The features described in this study make these systems promising tools for metabolic engineering and biotechnology in Actinobacteria.en
dc.language.isoenen
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/613877en
dc.rightsopenAccessen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleDual control system - A novel scaffolding architecture of an inducible regulatory device for the precise regulation of gene expression.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Institut für Pharmazeutische Forschung Saarland, Universitätscampus E8.1, 66123 Saarbrücken, Germany.en
dc.identifier.journalMetabolic engineeringen

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