A novel protein quality control mechanism contributes to heat shock resistance of worldwide-distributed Pseudomonas aeruginosa clone C strains.

2.50
Hdl Handle:
http://hdl.handle.net/10033/619838
Title:
A novel protein quality control mechanism contributes to heat shock resistance of worldwide-distributed Pseudomonas aeruginosa clone C strains.
Authors:
Lee, Changhan; Wigren, Edvard; Trček, Janja; Peters, Verena; Kim, Jihong; Hasni, Muhammad Sharif; Nimtz, Manfred; Lindqvist, Ylva; Park, Chankyu; Curth, Ute; Lünsdorf, Heinrich; Römling, Ute
Abstract:
Pseudomonas aeruginosa is a highly successful nosocomial pathogen capable of causing a wide variety of infections with clone C strains most prevalent worldwide. In this study, we initially characterize a molecular mechanism of survival unique to clone C strains. We identified a P. aeruginosa clone C-specific genomic island (PACGI-1) that contains the highly expressed small heat shock protein sHsp20c, the founding member of a novel subclass of class B bacterial small heat shock proteins. sHsp20c and adjacent gene products are involved in resistance against heat shock. Heat stable sHsp20c is unconventionally expressed in stationary phase in a wide temperature range from 20 to 42°C. Purified sHsp20c has characteristic features of small heat shock protein class B as it is monodisperse, forms sphere-like 24-meric oligomers and exhibits significant chaperone activity. As the P. aeruginosa clone C population is significantly more heat shock resistant than genetically unrelated P. aeruginosa strains without sHsp20c, the horizontally acquired shsp20c operon might contribute to the survival of worldwide-distributed clone C strains.
Affiliation:
Helmholtz Centre for infection research, Inhoffenstr. 7,38124 Braunschweig, Germany.
Citation:
A novel protein quality control mechanism contributes to heat shock resistance of worldwide-distributed Pseudomonas aeruginosa clone C strains. 2015, 17 (11):4511-26 Environ. Microbiol.
Journal:
Environmental microbiology
Issue Date:
Nov-2015
URI:
http://hdl.handle.net/10033/619838
DOI:
10.1111/1462-2920.12915
PubMed ID:
26014207
Type:
Article
Language:
en
ISSN:
1462-2920
Appears in Collections:
publications of the central unit for microscopy (ZEIM)

Full metadata record

DC FieldValue Language
dc.contributor.authorLee, Changhanen
dc.contributor.authorWigren, Edvarden
dc.contributor.authorTrček, Janjaen
dc.contributor.authorPeters, Verenaen
dc.contributor.authorKim, Jihongen
dc.contributor.authorHasni, Muhammad Sharifen
dc.contributor.authorNimtz, Manfreden
dc.contributor.authorLindqvist, Ylvaen
dc.contributor.authorPark, Chankyuen
dc.contributor.authorCurth, Uteen
dc.contributor.authorLünsdorf, Heinrichen
dc.contributor.authorRömling, Uteen
dc.date.accessioned2016-09-05T11:50:09Z-
dc.date.available2016-09-05T11:50:09Z-
dc.date.issued2015-11-
dc.identifier.citationA novel protein quality control mechanism contributes to heat shock resistance of worldwide-distributed Pseudomonas aeruginosa clone C strains. 2015, 17 (11):4511-26 Environ. Microbiol.en
dc.identifier.issn1462-2920-
dc.identifier.pmid26014207-
dc.identifier.doi10.1111/1462-2920.12915-
dc.identifier.urihttp://hdl.handle.net/10033/619838-
dc.description.abstractPseudomonas aeruginosa is a highly successful nosocomial pathogen capable of causing a wide variety of infections with clone C strains most prevalent worldwide. In this study, we initially characterize a molecular mechanism of survival unique to clone C strains. We identified a P. aeruginosa clone C-specific genomic island (PACGI-1) that contains the highly expressed small heat shock protein sHsp20c, the founding member of a novel subclass of class B bacterial small heat shock proteins. sHsp20c and adjacent gene products are involved in resistance against heat shock. Heat stable sHsp20c is unconventionally expressed in stationary phase in a wide temperature range from 20 to 42°C. Purified sHsp20c has characteristic features of small heat shock protein class B as it is monodisperse, forms sphere-like 24-meric oligomers and exhibits significant chaperone activity. As the P. aeruginosa clone C population is significantly more heat shock resistant than genetically unrelated P. aeruginosa strains without sHsp20c, the horizontally acquired shsp20c operon might contribute to the survival of worldwide-distributed clone C strains.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subject.meshAmino Acid Sequenceen
dc.subject.meshBase Sequenceen
dc.subject.meshCross Infectionen
dc.subject.meshDNA, Bacterialen
dc.subject.meshGenomic Islandsen
dc.subject.meshHeat-Shock Proteinsen
dc.subject.meshHeat-Shock Responseen
dc.subject.meshHot Temperatureen
dc.subject.meshMolecular Sequence Dataen
dc.subject.meshPseudomonas aeruginosaen
dc.subject.meshSequence Analysis, DNAen
dc.titleA novel protein quality control mechanism contributes to heat shock resistance of worldwide-distributed Pseudomonas aeruginosa clone C strains.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection research, Inhoffenstr. 7,38124 Braunschweig, Germany.en
dc.identifier.journalEnvironmental microbiologyen
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