aroA-Deficient Salmonella enterica Serovar Typhimurium Is More Than a Metabolically Attenuated Mutant.

2.50
Hdl Handle:
http://hdl.handle.net/10033/620109
Title:
aroA-Deficient Salmonella enterica Serovar Typhimurium Is More Than a Metabolically Attenuated Mutant.
Authors:
Felgner, Sebastian; Frahm, Michael; Kocijancic, Dino; Rohde, M ( 0000-0003-0522-3580 ) ; Eckweiler, Denitsa; Bielecka, Agata; Bueno, Emilio; Cava, Felipe; Abraham, Wolf-Rainer ( 0000-0002-2850-2649 ) ; Curtiss, Roy; Häussler, Susanne; Erhardt, Marc; Weiss, Siegfried ( 0000-0002-5276-8658 )
Abstract:
Recombinant attenuated Salmonella enterica serovar Typhimurium strains are believed to act as powerful live vaccine carriers that are able to elicit protection against various pathogens. Auxotrophic mutations, such as a deletion of aroA, are commonly introduced into such bacteria for attenuation without incapacitating immunostimulation. In this study, we describe the surprising finding that deletion of aroA dramatically increased the virulence of attenuated Salmonella in mouse models. Mutant bacteria lacking aroA elicited increased levels of the proinflammatory cytokine tumor necrosis factor alpha (TNF-α) after systemic application. A detailed genetic and phenotypic characterization in combination with transcriptomic and metabolic profiling demonstrated that ΔaroA mutants display pleiotropic alterations in cellular physiology and lipid and amino acid metabolism, as well as increased sensitivity to penicillin, complement, and phagocytic uptake. In concert with other immunomodulating mutations, deletion of aroA affected flagellin phase variation and gene expression of the virulence-associated genes arnT and ansB Finally, ΔaroA strains displayed significantly improved tumor therapeutic activity. These results highlight the importance of a functional shikimate pathway to control homeostatic bacterial physiology. They further highlight the great potential of ΔaroA-attenuated Salmonella for the development of vaccines and cancer therapies with important implications for host-pathogen interactions and translational medicine.
Citation:
aroA-Deficient Salmonella enterica Serovar Typhimurium Is More Than a Metabolically Attenuated Mutant. 2016, 7 (5): MBio
Journal:
mBio
Issue Date:
2016
URI:
http://hdl.handle.net/10033/620109
DOI:
10.1128/mBio.01220-16
PubMed ID:
27601574
Type:
Article
Language:
en
ISSN:
2150-7511
Appears in Collections:
publications of the research group molecular Immunology (MOLI)

Full metadata record

DC FieldValue Language
dc.contributor.authorFelgner, Sebastianen
dc.contributor.authorFrahm, Michaelen
dc.contributor.authorKocijancic, Dinoen
dc.contributor.authorRohde, Men
dc.contributor.authorEckweiler, Denitsaen
dc.contributor.authorBielecka, Agataen
dc.contributor.authorBueno, Emilioen
dc.contributor.authorCava, Felipeen
dc.contributor.authorAbraham, Wolf-Raineren
dc.contributor.authorCurtiss, Royen
dc.contributor.authorHäussler, Susanneen
dc.contributor.authorErhardt, Marcen
dc.contributor.authorWeiss, Siegfrieden
dc.date.accessioned2016-09-14T14:34:24Z-
dc.date.available2016-09-14T14:34:24Z-
dc.date.issued2016-
dc.identifier.citationaroA-Deficient Salmonella enterica Serovar Typhimurium Is More Than a Metabolically Attenuated Mutant. 2016, 7 (5): MBioen
dc.identifier.issn2150-7511-
dc.identifier.pmid27601574-
dc.identifier.doi10.1128/mBio.01220-16-
dc.identifier.urihttp://hdl.handle.net/10033/620109-
dc.description.abstractRecombinant attenuated Salmonella enterica serovar Typhimurium strains are believed to act as powerful live vaccine carriers that are able to elicit protection against various pathogens. Auxotrophic mutations, such as a deletion of aroA, are commonly introduced into such bacteria for attenuation without incapacitating immunostimulation. In this study, we describe the surprising finding that deletion of aroA dramatically increased the virulence of attenuated Salmonella in mouse models. Mutant bacteria lacking aroA elicited increased levels of the proinflammatory cytokine tumor necrosis factor alpha (TNF-α) after systemic application. A detailed genetic and phenotypic characterization in combination with transcriptomic and metabolic profiling demonstrated that ΔaroA mutants display pleiotropic alterations in cellular physiology and lipid and amino acid metabolism, as well as increased sensitivity to penicillin, complement, and phagocytic uptake. In concert with other immunomodulating mutations, deletion of aroA affected flagellin phase variation and gene expression of the virulence-associated genes arnT and ansB Finally, ΔaroA strains displayed significantly improved tumor therapeutic activity. These results highlight the importance of a functional shikimate pathway to control homeostatic bacterial physiology. They further highlight the great potential of ΔaroA-attenuated Salmonella for the development of vaccines and cancer therapies with important implications for host-pathogen interactions and translational medicine.en
dc.language.isoenen
dc.relation'info:eu-repo/grantAgreement/EC/FP7/334030en
dc.rightsopenAccessen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titlearoA-Deficient Salmonella enterica Serovar Typhimurium Is More Than a Metabolically Attenuated Mutant.en
dc.typeArticleen
dc.identifier.journalmBioen
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