Autologous co-culture of primary human alveolar macrophages and epithelial cells for investigating aerosol medicines. Part II: evaluation of IL-10-loaded microparticles for the treatment of lung inflammation.

2.50
Hdl Handle:
http://hdl.handle.net/10033/620580
Title:
Autologous co-culture of primary human alveolar macrophages and epithelial cells for investigating aerosol medicines. Part II: evaluation of IL-10-loaded microparticles for the treatment of lung inflammation.
Authors:
Hittinger, Marius; Mell, Nico Alexander; Huwer, Hanno; Loretz, Brigitta; Schneider-Daum, Nicole; Lehr, Claus Michael
Abstract:
Acute respiratory distress syndrome is linked to inflammatory processes in the human lung. The aim of this study was to mimic in vitro the treatment of lung inflammation by using a cell-based human autologous co-culture model. As a potential trial medication, we developed a pulmonary dry powder formulation loaded with interleukin-10 (IL-10), a potent anti-inflammatory cytokine. The inflammatory immune response was stimulated by lipopolysaccharide. The co-culture was combined with the Pharmaceutical Aerosol Deposition Device on Cell Cultures )PADDOCC), to deposit the IL-10-loaded microparticles on the inflamed co-culture model at the air-liquid interface. This treatment significantly reduced the secretion of interleukin-6 and tumour necrosis factor, as compared to the deposition of placebo (unloaded) particles. Our results show that the alveolar co-culture model, in combination with a deposition device such as the PADDOCC, may serve as a powerful tool for testing the safety and efficacy of dry powder formulations for pulmonary drug delivery.
Affiliation:
Helmholtz-Institute for Pharmaceutical Research Saarland,Universitätscampus E8.1, 66123 Saarbrücken, Germany.
Citation:
Autologous co-culture of primary human alveolar macrophages and epithelial cells for investigating aerosol medicines. Part II: evaluation of IL-10-loaded microparticles for the treatment of lung inflammation. 2016, 44 (4):349-360 Altern Lab Anim
Journal:
Alternatives to laboratory animals : ATLA
Issue Date:
Sep-2016
URI:
http://hdl.handle.net/10033/620580
PubMed ID:
27685186
Type:
Article
Language:
en
ISSN:
0261-1929
Appears in Collections:
publications of the department drug delivery ([TC] DDEL)

Full metadata record

DC FieldValue Language
dc.contributor.authorHittinger, Mariusen
dc.contributor.authorMell, Nico Alexanderen
dc.contributor.authorHuwer, Hannoen
dc.contributor.authorLoretz, Brigittaen
dc.contributor.authorSchneider-Daum, Nicoleen
dc.contributor.authorLehr, Claus Michaelen
dc.date.accessioned2016-11-15T13:09:07Z-
dc.date.available2016-11-15T13:09:07Z-
dc.date.issued2016-09-
dc.identifier.citationAutologous co-culture of primary human alveolar macrophages and epithelial cells for investigating aerosol medicines. Part II: evaluation of IL-10-loaded microparticles for the treatment of lung inflammation. 2016, 44 (4):349-360 Altern Lab Animen
dc.identifier.issn0261-1929-
dc.identifier.pmid27685186-
dc.identifier.urihttp://hdl.handle.net/10033/620580-
dc.description.abstractAcute respiratory distress syndrome is linked to inflammatory processes in the human lung. The aim of this study was to mimic in vitro the treatment of lung inflammation by using a cell-based human autologous co-culture model. As a potential trial medication, we developed a pulmonary dry powder formulation loaded with interleukin-10 (IL-10), a potent anti-inflammatory cytokine. The inflammatory immune response was stimulated by lipopolysaccharide. The co-culture was combined with the Pharmaceutical Aerosol Deposition Device on Cell Cultures )PADDOCC), to deposit the IL-10-loaded microparticles on the inflamed co-culture model at the air-liquid interface. This treatment significantly reduced the secretion of interleukin-6 and tumour necrosis factor, as compared to the deposition of placebo (unloaded) particles. Our results show that the alveolar co-culture model, in combination with a deposition device such as the PADDOCC, may serve as a powerful tool for testing the safety and efficacy of dry powder formulations for pulmonary drug delivery.en
dc.languageENG-
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleAutologous co-culture of primary human alveolar macrophages and epithelial cells for investigating aerosol medicines. Part II: evaluation of IL-10-loaded microparticles for the treatment of lung inflammation.en
dc.typeArticleen
dc.contributor.departmentHelmholtz-Institute for Pharmaceutical Research Saarland,Universitätscampus E8.1, 66123 Saarbrücken, Germany.en
dc.identifier.journalAlternatives to laboratory animals : ATLAen
This item is licensed under a Creative Commons License
Creative Commons
All Items in HZI are protected by copyright, with all rights reserved, unless otherwise indicated.