Damage of Streptococcus mutans biofilms by carolacton, a secondary metabolite from the myxobacterium Sorangium cellulosum

2.50
Hdl Handle:
http://hdl.handle.net/10033/620720
Title:
Damage of Streptococcus mutans biofilms by carolacton, a secondary metabolite from the myxobacterium Sorangium cellulosum
Authors:
Kunze, Brigitte; Reck, Michael; Dötsch, Andreas; Lemme, André; Schummer, Dietmar; Irschik, Herbert; Steinmetz, Heinrich; Wagner-Döbler, Irene
Abstract:
Abstract Background Streptococcus mutans is a major pathogen in human dental caries. One of its important virulence properties is the ability to form biofilms (dental plaque) on tooth surfaces. Eradication of such biofilms is extremely difficult. We therefore screened a library of secondary metabolites from myxobacteria for their ability to damage biofilms of S. mutans. Results Here we show that carolacton, a secondary metabolite isolated from Sorangium cellulosum, has high antibacterial activity against biofilms of S. mutans. Planktonic growth of bacteria was only slightly impaired and no acute cytotoxicity against mouse fibroblasts could be observed. Carolacton caused death of S. mutans biofilm cells, elongation of cell chains, and changes in cell morphology. At a concentration of 10 nM carolacton, biofilm damage was already at 35% under anaerobic conditions. A knock-out mutant for comD, encoding a histidine kinase specific for the competence stimulating peptide (CSP), was slightly less sensitive to carolacton than the wildtype. Expression of the competence related alternate sigma factor ComX was strongly reduced by carolacton, as determined by a pcomX luciferase reporter strain. Conclusions Carolacton possibly interferes with the density dependent signalling systems in S. mutans and may represent a novel approach for the prevention of dental caries.
Citation:
BMC Microbiology. 2010 Jul 26;10(1):199
Issue Date:
26-Jul-2010
URI:
http://dx.doi.org/10.1186/1471-2180-10-199; http://hdl.handle.net/10033/620720
Type:
Journal Article
Appears in Collections:
collections of the research group microbial communication (KOM)

Full metadata record

DC FieldValue Language
dc.contributor.authorKunze, Brigitteen
dc.contributor.authorReck, Michaelen
dc.contributor.authorDötsch, Andreasen
dc.contributor.authorLemme, Andréen
dc.contributor.authorSchummer, Dietmaren
dc.contributor.authorIrschik, Herberten
dc.contributor.authorSteinmetz, Heinrichen
dc.contributor.authorWagner-Döbler, Ireneen
dc.date.accessioned2017-01-17T10:41:51Z-
dc.date.available2017-01-17T10:41:51Z-
dc.date.issued2010-07-26en
dc.identifier.citationBMC Microbiology. 2010 Jul 26;10(1):199en
dc.identifier.urihttp://dx.doi.org/10.1186/1471-2180-10-199en
dc.identifier.urihttp://hdl.handle.net/10033/620720-
dc.description.abstractAbstract Background Streptococcus mutans is a major pathogen in human dental caries. One of its important virulence properties is the ability to form biofilms (dental plaque) on tooth surfaces. Eradication of such biofilms is extremely difficult. We therefore screened a library of secondary metabolites from myxobacteria for their ability to damage biofilms of S. mutans. Results Here we show that carolacton, a secondary metabolite isolated from Sorangium cellulosum, has high antibacterial activity against biofilms of S. mutans. Planktonic growth of bacteria was only slightly impaired and no acute cytotoxicity against mouse fibroblasts could be observed. Carolacton caused death of S. mutans biofilm cells, elongation of cell chains, and changes in cell morphology. At a concentration of 10 nM carolacton, biofilm damage was already at 35% under anaerobic conditions. A knock-out mutant for comD, encoding a histidine kinase specific for the competence stimulating peptide (CSP), was slightly less sensitive to carolacton than the wildtype. Expression of the competence related alternate sigma factor ComX was strongly reduced by carolacton, as determined by a pcomX luciferase reporter strain. Conclusions Carolacton possibly interferes with the density dependent signalling systems in S. mutans and may represent a novel approach for the prevention of dental caries.en
dc.titleDamage of Streptococcus mutans biofilms by carolacton, a secondary metabolite from the myxobacterium Sorangium cellulosumen
dc.typeJournal Articleen
dc.language.rfc3066enen
dc.rights.holderKunze et al.en
dc.date.updated2015-09-04T08:28:51Zen
All Items in HZI are protected by copyright, with all rights reserved, unless otherwise indicated.