Multiple antibody targets on herpes B glycoproteins B and D identified by screening sera of infected rhesus macaques with peptide microarrays.

2.50
Hdl Handle:
http://hdl.handle.net/10033/620740
Title:
Multiple antibody targets on herpes B glycoproteins B and D identified by screening sera of infected rhesus macaques with peptide microarrays.
Authors:
Hotop, Sven-Kevin; Abd El Wahed, Ahmed; Beutling, Ulrike; Jentsch, Dieter; Motzkus, Dirk; Frank, Ronald; Hunsmann, Gerhard; Stahl-Hennig, Christiane; Fritz, Hans-Joachim
Abstract:
Herpes B virus (or Herpesvirus simiae or Macacine herpesvirus 1) is endemic in many populations of macaques, both in the wild and in captivity. The virus elicits only mild clinical symptoms (if any) in monkeys, but can be transmitted by various routes, most commonly via bites, to humans where it causes viral encephalitis with a high mortality rate. Hence, herpes B constitutes a considerable occupational hazard for animal caretakers, veterinarians and laboratory personnel. Efforts are therefore being made to reduce the risk of zoonotic infection and to improve prognosis after accidental exposure. Among the measures envisaged are serological surveillance of monkey colonies and specific diagnosis of herpes B zoonosis against a background of antibodies recognizing the closely related human herpes simplex virus (HSV). 422 pentadecapeptides covering, in an overlapping fashion, the entire amino acid sequences of herpes B proteins gB and gD were synthesized and immobilized on glass slides. Antibodies present in monkey sera that bind to subsets of the peptide collection were detected by microserological techniques. With 42 different rhesus macaque sera, 114 individual responses to 18 different antibody target regions (ATRs) were recorded, 17 of which had not been described earlier. This finding may pave the way for a peptide-based, herpes B specific serological diagnostic test.
Affiliation:
Helmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.
Citation:
Multiple antibody targets on herpes B glycoproteins B and D identified by screening sera of infected rhesus macaques with peptide microarrays. 2014, 9 (1):e86857 PLoS ONE
Journal:
PloS one
Issue Date:
2014
URI:
http://hdl.handle.net/10033/620740
DOI:
10.1371/journal.pone.0086857
PubMed ID:
24497986
Type:
Article
Language:
en
ISSN:
1932-6203
Appears in Collections:
Publications of the research group Chemical Biology (CBIO)

Full metadata record

DC FieldValue Language
dc.contributor.authorHotop, Sven-Kevinen
dc.contributor.authorAbd El Wahed, Ahmeden
dc.contributor.authorBeutling, Ulrikeen
dc.contributor.authorJentsch, Dieteren
dc.contributor.authorMotzkus, Dirken
dc.contributor.authorFrank, Ronalden
dc.contributor.authorHunsmann, Gerharden
dc.contributor.authorStahl-Hennig, Christianeen
dc.contributor.authorFritz, Hans-Joachimen
dc.date.accessioned2017-01-23T14:19:39Z-
dc.date.available2017-01-23T14:19:39Z-
dc.date.issued2014-
dc.identifier.citationMultiple antibody targets on herpes B glycoproteins B and D identified by screening sera of infected rhesus macaques with peptide microarrays. 2014, 9 (1):e86857 PLoS ONEen
dc.identifier.issn1932-6203-
dc.identifier.pmid24497986-
dc.identifier.doi10.1371/journal.pone.0086857-
dc.identifier.urihttp://hdl.handle.net/10033/620740-
dc.description.abstractHerpes B virus (or Herpesvirus simiae or Macacine herpesvirus 1) is endemic in many populations of macaques, both in the wild and in captivity. The virus elicits only mild clinical symptoms (if any) in monkeys, but can be transmitted by various routes, most commonly via bites, to humans where it causes viral encephalitis with a high mortality rate. Hence, herpes B constitutes a considerable occupational hazard for animal caretakers, veterinarians and laboratory personnel. Efforts are therefore being made to reduce the risk of zoonotic infection and to improve prognosis after accidental exposure. Among the measures envisaged are serological surveillance of monkey colonies and specific diagnosis of herpes B zoonosis against a background of antibodies recognizing the closely related human herpes simplex virus (HSV). 422 pentadecapeptides covering, in an overlapping fashion, the entire amino acid sequences of herpes B proteins gB and gD were synthesized and immobilized on glass slides. Antibodies present in monkey sera that bind to subsets of the peptide collection were detected by microserological techniques. With 42 different rhesus macaque sera, 114 individual responses to 18 different antibody target regions (ATRs) were recorded, 17 of which had not been described earlier. This finding may pave the way for a peptide-based, herpes B specific serological diagnostic test.en
dc.language.isoenen
dc.subject.meshAmino Acid Sequenceen
dc.subject.meshAnimalsen
dc.subject.meshAntibodies, Viralen
dc.subject.meshEnzyme-Linked Immunosorbent Assayen
dc.subject.meshEpitopesen
dc.subject.meshHerpesviridae Infectionsen
dc.subject.meshHerpesvirus 1, Cercopithecineen
dc.subject.meshHost-Pathogen Interactionsen
dc.subject.meshHumansen
dc.subject.meshMacaca mulattaen
dc.subject.meshModels, Molecularen
dc.subject.meshMolecular Sequence Dataen
dc.subject.meshPeptidesen
dc.subject.meshProtein Array Analysisen
dc.subject.meshProtein Structure, Tertiaryen
dc.subject.meshSensitivity and Specificityen
dc.subject.meshSequence Homology, Amino Aciden
dc.subject.meshSimplexvirusen
dc.subject.meshViral Envelope Proteinsen
dc.subject.meshZoonosesen
dc.titleMultiple antibody targets on herpes B glycoproteins B and D identified by screening sera of infected rhesus macaques with peptide microarrays.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.en
dc.identifier.journalPloS oneen

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