Virus-like particle production with yeast: ultrastructural and immunocytochemical insights into Pichia pastoris producing high levels of the hepatitis B surface antigen.

2.50
Hdl Handle:
http://hdl.handle.net/10033/620742
Title:
Virus-like particle production with yeast: ultrastructural and immunocytochemical insights into Pichia pastoris producing high levels of the hepatitis B surface antigen.
Authors:
Lünsdorf, Heinrich; Gurramkonda, Chandrasekhar; Adnan, Ahmad; Khanna, Navin; Rinas, Ursula
Abstract:
A protective immune response against Hepatitis B infection can be obtained through the administration of a single viral polypeptide, the Hepatitis B surface antigen (HBsAg). Thus, the Hepatitis B vaccine is generated through the utilization of recombinant DNA technology, preferentially by using yeast-based expression systems. However, the polypeptide needs to assemble into spherical particles, so-called virus-like particles (VLPs), to elicit the required protective immune response. So far, no clear evidence has been presented showing whether HBsAg assembles in vivo inside the yeast cell into VLPs or later in vitro during down-stream processing and purification.
Affiliation:
Helmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.
Citation:
Virus-like particle production with yeast: ultrastructural and immunocytochemical insights into Pichia pastoris producing high levels of the hepatitis B surface antigen. 2011, 10:48 Microb. Cell Fact.
Journal:
Microbial cell factories
Issue Date:
26-Jun-2011
URI:
http://hdl.handle.net/10033/620742
DOI:
10.1186/1475-2859-10-48
PubMed ID:
21703024
Type:
Article
Language:
en
ISSN:
1475-2859
Appears in Collections:
publications of the research group vaccinology and applied microbiology (VAC)

Full metadata record

DC FieldValue Language
dc.contributor.authorLünsdorf, Heinrichen
dc.contributor.authorGurramkonda, Chandrasekharen
dc.contributor.authorAdnan, Ahmaden
dc.contributor.authorKhanna, Navinen
dc.contributor.authorRinas, Ursulaen
dc.date.accessioned2017-01-24T14:40:32Z-
dc.date.available2017-01-24T14:40:32Z-
dc.date.issued2011-06-26-
dc.identifier.citationVirus-like particle production with yeast: ultrastructural and immunocytochemical insights into Pichia pastoris producing high levels of the hepatitis B surface antigen. 2011, 10:48 Microb. Cell Fact.en
dc.identifier.issn1475-2859-
dc.identifier.pmid21703024-
dc.identifier.doi10.1186/1475-2859-10-48-
dc.identifier.urihttp://hdl.handle.net/10033/620742-
dc.description.abstractA protective immune response against Hepatitis B infection can be obtained through the administration of a single viral polypeptide, the Hepatitis B surface antigen (HBsAg). Thus, the Hepatitis B vaccine is generated through the utilization of recombinant DNA technology, preferentially by using yeast-based expression systems. However, the polypeptide needs to assemble into spherical particles, so-called virus-like particles (VLPs), to elicit the required protective immune response. So far, no clear evidence has been presented showing whether HBsAg assembles in vivo inside the yeast cell into VLPs or later in vitro during down-stream processing and purification.en
dc.language.isoenen
dc.subject.meshAldehyde Oxidaseen
dc.subject.meshEndoplasmic Reticulumen
dc.subject.meshHepatitis B Surface Antigensen
dc.subject.meshImmunohistochemistryen
dc.subject.meshMethanolen
dc.subject.meshMicroscopy, Electron, Transmissionen
dc.subject.meshPichiaen
dc.subject.meshVaccines, Syntheticen
dc.subject.meshVaccines, Virus-Like Particleen
dc.titleVirus-like particle production with yeast: ultrastructural and immunocytochemical insights into Pichia pastoris producing high levels of the hepatitis B surface antigen.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.en
dc.identifier.journalMicrobial cell factoriesen

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