2.50
Hdl Handle:
http://hdl.handle.net/10033/620778
Title:
Insights into naturally minimised Streptomyces albus J1074 genome
Authors:
Zaburannyi, Nestor; Rabyk, Mariia; Ostash, Bohdan; Fedorenko, Victor; Luzhetskyy, Andriy ( 0000-0001-6497-0047 )
Abstract:
Abstract Background The Streptomyces albus J1074 strain is one of the most widely used chassis for the heterologous production of bioactive natural products. The fast growth and an efficient genetic system make this strain an attractive model for expressing cryptic biosynthetic pathways to aid drug discovery. Results To improve its capabilities for the heterologous expression of biosynthetic gene clusters, the complete genomic sequence of S. albus J1074 was obtained. With a size of 6,841,649 bp, coding for 5,832 genes, its genome is the smallest within the genus streptomycetes. Genome analysis revealed a strong tendency to reduce the number of genetic duplicates. The whole transcriptomes were sequenced at different time points to identify the early metabolic switch from the exponential to the stationary phase in S. albus J1074. Conclusions S. albus J1074 carries the smallest genome among the completely sequenced species of the genus Streptomyces. The detailed genome and transcriptome analysis discloses its capability to serve as a premium host for the heterologous production of natural products. Moreover, the genome revealed 22 additional putative secondary metabolite gene clusters that reinforce the strain’s potential for natural product synthesis.
Citation:
BMC Genomics. 2014 Feb 05;15(1):97
Issue Date:
5-Feb-2014
URI:
http://dx.doi.org/10.1186/1471-2164-15-97; http://hdl.handle.net/10033/620778
Type:
Journal Article
Appears in Collections:
publications of the research group metabolic engineering of Actinomycetes ([HIPS]AMEG)

Full metadata record

DC FieldValue Language
dc.contributor.authorZaburannyi, Nestoren
dc.contributor.authorRabyk, Mariiaen
dc.contributor.authorOstash, Bohdanen
dc.contributor.authorFedorenko, Victoren
dc.contributor.authorLuzhetskyy, Andriyen
dc.date.accessioned2017-01-27T10:45:08Z-
dc.date.available2017-01-27T10:45:08Z-
dc.date.issued2014-02-05en
dc.identifier.citationBMC Genomics. 2014 Feb 05;15(1):97en
dc.identifier.urihttp://dx.doi.org/10.1186/1471-2164-15-97en
dc.identifier.urihttp://hdl.handle.net/10033/620778-
dc.description.abstractAbstract Background The Streptomyces albus J1074 strain is one of the most widely used chassis for the heterologous production of bioactive natural products. The fast growth and an efficient genetic system make this strain an attractive model for expressing cryptic biosynthetic pathways to aid drug discovery. Results To improve its capabilities for the heterologous expression of biosynthetic gene clusters, the complete genomic sequence of S. albus J1074 was obtained. With a size of 6,841,649 bp, coding for 5,832 genes, its genome is the smallest within the genus streptomycetes. Genome analysis revealed a strong tendency to reduce the number of genetic duplicates. The whole transcriptomes were sequenced at different time points to identify the early metabolic switch from the exponential to the stationary phase in S. albus J1074. Conclusions S. albus J1074 carries the smallest genome among the completely sequenced species of the genus Streptomyces. The detailed genome and transcriptome analysis discloses its capability to serve as a premium host for the heterologous production of natural products. Moreover, the genome revealed 22 additional putative secondary metabolite gene clusters that reinforce the strain’s potential for natural product synthesis.en
dc.titleInsights into naturally minimised Streptomyces albus J1074 genomeen
dc.typeJournal Articleen
dc.language.rfc3066enen
dc.rights.holderZaburannyi et al.; licensee BioMed Central Ltd.en
dc.date.updated2015-09-04T08:27:47Zen
All Items in HZI are protected by copyright, with all rights reserved, unless otherwise indicated.