2.50
Hdl Handle:
http://hdl.handle.net/10033/620781
Title:
Elevated expression of VEGFR-3 in lymphatic endothelial cells from lymphangiomas
Authors:
Norgall, Susanne; Papoutsi, Maria; Rössler, Jochen; Schweigerer, Lothar; Wilting, Jörg; Weich, Herbert A
Abstract:
Abstract Background Lymphangiomas are neoplasias of childhood. Their etiology is unknown and a causal therapy does not exist. The recent discovery of highly specific markers for lymphatic endothelial cells (LECs) has permitted their isolation and characterization, but expression levels and stability of molecular markers on LECs from healthy and lymphangioma tissues have not been studied yet. We addressed this problem by profiling LECs from normal dermis and two children suffering from lymphangioma, and also compared them with blood endothelial cells (BECs) from umbilical vein, aorta and myometrial microvessels. Methods Lymphangioma tissue samples were obtained from two young patients suffering from lymphangioma in the axillary and upper arm region. Initially isolated with anti-CD31 (PECAM-1) antibodies, the cells were separated by FACS sorting and magnetic beads using anti-podoplanin and/or LYVE-1 antibodies. Characterization was performed by FACS analysis, immunofluorescence staining, ELISA and micro-array gene analysis. Results LECs from foreskin and lymphangioma had an almost identical pattern of lymphendothelial markers such as podoplanin, Prox1, reelin, cMaf and integrin-α1 and -α9. However, LYVE-1 was down-regulated and VEGFR-2 and R-3 were up-regulated in lymphangiomas. Prox1 was constantly expressed in LECs but not in any of the BECs. Conclusion LECs from different sources express slightly variable molecular markers, but can always be distinguished from BECs by their Prox1 expression. High levels of VEGFR-3 and -2 seem to contribute to the etiology of lymphangiomas.
Citation:
BMC Cancer. 2007 Jun 21;7(1):105
Issue Date:
21-Jun-2007
URI:
http://dx.doi.org/10.1186/1471-2407-7-105; http://hdl.handle.net/10033/620781
Type:
Journal Article
Appears in Collections:
Publications of the research group Chemical Biology (CBIO)

Full metadata record

DC FieldValue Language
dc.contributor.authorNorgall, Susanneen
dc.contributor.authorPapoutsi, Mariaen
dc.contributor.authorRössler, Jochenen
dc.contributor.authorSchweigerer, Lotharen
dc.contributor.authorWilting, Jörgen
dc.contributor.authorWeich, Herbert Aen
dc.date.accessioned2017-01-27T11:33:16Z-
dc.date.available2017-01-27T11:33:16Z-
dc.date.issued2007-06-21en
dc.identifier.citationBMC Cancer. 2007 Jun 21;7(1):105en
dc.identifier.urihttp://dx.doi.org/10.1186/1471-2407-7-105en
dc.identifier.urihttp://hdl.handle.net/10033/620781-
dc.description.abstractAbstract Background Lymphangiomas are neoplasias of childhood. Their etiology is unknown and a causal therapy does not exist. The recent discovery of highly specific markers for lymphatic endothelial cells (LECs) has permitted their isolation and characterization, but expression levels and stability of molecular markers on LECs from healthy and lymphangioma tissues have not been studied yet. We addressed this problem by profiling LECs from normal dermis and two children suffering from lymphangioma, and also compared them with blood endothelial cells (BECs) from umbilical vein, aorta and myometrial microvessels. Methods Lymphangioma tissue samples were obtained from two young patients suffering from lymphangioma in the axillary and upper arm region. Initially isolated with anti-CD31 (PECAM-1) antibodies, the cells were separated by FACS sorting and magnetic beads using anti-podoplanin and/or LYVE-1 antibodies. Characterization was performed by FACS analysis, immunofluorescence staining, ELISA and micro-array gene analysis. Results LECs from foreskin and lymphangioma had an almost identical pattern of lymphendothelial markers such as podoplanin, Prox1, reelin, cMaf and integrin-α1 and -α9. However, LYVE-1 was down-regulated and VEGFR-2 and R-3 were up-regulated in lymphangiomas. Prox1 was constantly expressed in LECs but not in any of the BECs. Conclusion LECs from different sources express slightly variable molecular markers, but can always be distinguished from BECs by their Prox1 expression. High levels of VEGFR-3 and -2 seem to contribute to the etiology of lymphangiomas.en
dc.titleElevated expression of VEGFR-3 in lymphatic endothelial cells from lymphangiomasen
dc.typeJournal Articleen
dc.language.rfc3066enen
dc.rights.holderNorgall et al.en
dc.date.updated2015-09-04T08:27:25Zen
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