2.50
Hdl Handle:
http://hdl.handle.net/10033/620782
Title:
Genetic variability of mutans streptococci revealed by wide whole-genome sequencing
Authors:
Song, Lifu; Wang, Wei; Conrads, Georg; Rheinberg, Anke; Sztajer, Helena; Reck, Michael; Wagner-Döbler, Irene; Zeng, An-Ping
Abstract:
Abstract Background Mutans streptococci are a group of bacteria significantly contributing to tooth decay. Their genetic variability is however still not well understood. Results Genomes of 6 clinical S. mutans isolates of different origins, one isolate of S. sobrinus (DSM 20742) and one isolate of S. ratti (DSM 20564) were sequenced and comparatively analyzed. Genome alignment revealed a mosaic-like structure of genome arrangement. Genes related to pathogenicity are found to have high variations among the strains, whereas genes for oxidative stress resistance are well conserved, indicating the importance of this trait in the dental biofilm community. Analysis of genome-scale metabolic networks revealed significant differences in 42 pathways. A striking dissimilarity is the unique presence of two lactate oxidases in S. sobrinus DSM 20742, probably indicating an unusual capability of this strain in producing H2O2 and expanding its ecological niche. In addition, lactate oxidases may form with other enzymes a novel energetic pathway in S. sobrinus DSM 20742 that can remedy its deficiency in citrate utilization pathway. Using 67 S. mutans genomes currently available including the strains sequenced in this study, we estimates the theoretical core genome size of S. mutans, and performed modeling of S. mutans pan-genome by applying different fitting models. An “open” pan-genome was inferred. Conclusions The comparative genome analyses revealed diversities in the mutans streptococci group, especially with respect to the virulence related genes and metabolic pathways. The results are helpful for better understanding the evolution and adaptive mechanisms of these oral pathogen microorganisms and for combating them.
Citation:
BMC Genomics. 2013 Jun 28;14(1):430
Issue Date:
28-Jun-2013
URI:
http://dx.doi.org/10.1186/1471-2164-14-430; http://hdl.handle.net/10033/620782
Type:
Journal Article
Appears in Collections:
collections of the research group microbial communication (KOM)

Full metadata record

DC FieldValue Language
dc.contributor.authorSong, Lifuen
dc.contributor.authorWang, Weien
dc.contributor.authorConrads, Georgen
dc.contributor.authorRheinberg, Ankeen
dc.contributor.authorSztajer, Helenaen
dc.contributor.authorReck, Michaelen
dc.contributor.authorWagner-Döbler, Ireneen
dc.contributor.authorZeng, An-Pingen
dc.date.accessioned2017-01-27T11:34:55Z-
dc.date.available2017-01-27T11:34:55Z-
dc.date.issued2013-06-28en
dc.identifier.citationBMC Genomics. 2013 Jun 28;14(1):430en
dc.identifier.urihttp://dx.doi.org/10.1186/1471-2164-14-430en
dc.identifier.urihttp://hdl.handle.net/10033/620782-
dc.description.abstractAbstract Background Mutans streptococci are a group of bacteria significantly contributing to tooth decay. Their genetic variability is however still not well understood. Results Genomes of 6 clinical S. mutans isolates of different origins, one isolate of S. sobrinus (DSM 20742) and one isolate of S. ratti (DSM 20564) were sequenced and comparatively analyzed. Genome alignment revealed a mosaic-like structure of genome arrangement. Genes related to pathogenicity are found to have high variations among the strains, whereas genes for oxidative stress resistance are well conserved, indicating the importance of this trait in the dental biofilm community. Analysis of genome-scale metabolic networks revealed significant differences in 42 pathways. A striking dissimilarity is the unique presence of two lactate oxidases in S. sobrinus DSM 20742, probably indicating an unusual capability of this strain in producing H2O2 and expanding its ecological niche. In addition, lactate oxidases may form with other enzymes a novel energetic pathway in S. sobrinus DSM 20742 that can remedy its deficiency in citrate utilization pathway. Using 67 S. mutans genomes currently available including the strains sequenced in this study, we estimates the theoretical core genome size of S. mutans, and performed modeling of S. mutans pan-genome by applying different fitting models. An “open” pan-genome was inferred. Conclusions The comparative genome analyses revealed diversities in the mutans streptococci group, especially with respect to the virulence related genes and metabolic pathways. The results are helpful for better understanding the evolution and adaptive mechanisms of these oral pathogen microorganisms and for combating them.en
dc.titleGenetic variability of mutans streptococci revealed by wide whole-genome sequencingen
dc.typeJournal Articleen
dc.language.rfc3066enen
dc.rights.holderSong et al.; licensee BioMed Central Ltd.en
dc.date.updated2015-09-04T08:27:14Zen
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