2.50
Hdl Handle:
http://hdl.handle.net/10033/620836
Title:
Soil myxobacteria as a potential source of polyketide-peptide substances.
Authors:
Charousová, Ivana; Steinmetz, Heinrich; Medo, Juraj; Javoreková, Soňa; Wink, Joachim
Abstract:
Myxobacteria, a group of antimicrobial producing bacteria, have been successfully cultured and characterized from ten soil samples collected from different parts of Slovakia. A total of 79 myxobacteria belonging to four genera (Myxococcus, Corallococcus, Sorangium, and Polyangium) were isolated based on aspects of their life cycle. Twenty-five of them were purified, fermented, and screened for antimicrobial activities against 11 test microorganisms. Results indicated that crude extracts showed more significant activities against Gram-positive than against Gram-negative bacteria or fungi. Based on a higher degree and broader range of antimicrobial production, the two most potential extracts (K9-5, V3-1) were selected for HPLC fractionation against Micrococcus luteus and Staphylococcus aureus and LC/MS analysis of potential antibiotic metabolites. The analysis resulted in the identification of polyketide-peptide antibiotics, namely corallopyronin A and B (K9-5) and myxalamid B and C (V3-1), which were responsible for important Gram-positive activity in the observed strains. A sequence similarity search through BLAST revealed that these strains showed the highest sequence similarity to Corallococcus coralloides (K9-5, NCBI accession number KX256198) and Myxococcus xanthus (V3-1, NCBI accession number KX256197). Although screening of myxobacteria is laborious, due to difficulties in isolating cultures, this research represented the first report covering the isolation and cultivation of this challenging bacterial group from Slovakian soils as well as the screening of their antimicrobial activity, cultural identification, and secondary metabolite identification.
Affiliation:
Helmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.
Citation:
Soil myxobacteria as a potential source of polyketide-peptide substances. 2017 Folia Microbiol. (Praha)
Journal:
Folia microbiologica
Issue Date:
4-Feb-2017
URI:
http://hdl.handle.net/10033/620836
DOI:
10.1007/s12223-017-0502-2
PubMed ID:
28161814
Type:
Article
Language:
en
ISSN:
1874-9356
Appears in Collections:
publications of the research group microbial strain collection (MISG)

Full metadata record

DC FieldValue Language
dc.contributor.authorCharousová, Ivanaen
dc.contributor.authorSteinmetz, Heinrichen
dc.contributor.authorMedo, Jurajen
dc.contributor.authorJavoreková, Soňaen
dc.contributor.authorWink, Joachimen
dc.date.accessioned2017-02-22T15:05:32Z-
dc.date.available2017-02-22T15:05:32Z-
dc.date.issued2017-02-04-
dc.identifier.citationSoil myxobacteria as a potential source of polyketide-peptide substances. 2017 Folia Microbiol. (Praha)en
dc.identifier.issn1874-9356-
dc.identifier.pmid28161814-
dc.identifier.doi10.1007/s12223-017-0502-2-
dc.identifier.urihttp://hdl.handle.net/10033/620836-
dc.description.abstractMyxobacteria, a group of antimicrobial producing bacteria, have been successfully cultured and characterized from ten soil samples collected from different parts of Slovakia. A total of 79 myxobacteria belonging to four genera (Myxococcus, Corallococcus, Sorangium, and Polyangium) were isolated based on aspects of their life cycle. Twenty-five of them were purified, fermented, and screened for antimicrobial activities against 11 test microorganisms. Results indicated that crude extracts showed more significant activities against Gram-positive than against Gram-negative bacteria or fungi. Based on a higher degree and broader range of antimicrobial production, the two most potential extracts (K9-5, V3-1) were selected for HPLC fractionation against Micrococcus luteus and Staphylococcus aureus and LC/MS analysis of potential antibiotic metabolites. The analysis resulted in the identification of polyketide-peptide antibiotics, namely corallopyronin A and B (K9-5) and myxalamid B and C (V3-1), which were responsible for important Gram-positive activity in the observed strains. A sequence similarity search through BLAST revealed that these strains showed the highest sequence similarity to Corallococcus coralloides (K9-5, NCBI accession number KX256198) and Myxococcus xanthus (V3-1, NCBI accession number KX256197). Although screening of myxobacteria is laborious, due to difficulties in isolating cultures, this research represented the first report covering the isolation and cultivation of this challenging bacterial group from Slovakian soils as well as the screening of their antimicrobial activity, cultural identification, and secondary metabolite identification.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleSoil myxobacteria as a potential source of polyketide-peptide substances.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.en
dc.identifier.journalFolia microbiologicaen

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