Development of a genus-specific next generation sequencing approach for sensitive and quantitative determination of the Legionella microbiome in freshwater systems.

2.50
Hdl Handle:
http://hdl.handle.net/10033/620890
Title:
Development of a genus-specific next generation sequencing approach for sensitive and quantitative determination of the Legionella microbiome in freshwater systems.
Authors:
Pereira, Rui P A; Peplies, Jörg; Brettar, Ingrid; Höfle, Manfred G
Abstract:
Next Generation Sequencing (NGS) has revolutionized the analysis of natural and man-made microbial communities by using universal primers for bacteria in a PCR based approach targeting the 16S rRNA gene. In our study we narrowed primer specificity to a single, monophyletic genus because for many questions in microbiology only a specific part of the whole microbiome is of interest. We have chosen the genus Legionella, comprising more than 20 pathogenic species, due to its high relevance for water-based respiratory infections.
Affiliation:
Helmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.
Citation:
Development of a genus-specific next generation sequencing approach for sensitive and quantitative determination of the Legionella microbiome in freshwater systems. 2017, 17 (1):79 BMC Microbiol.
Journal:
BMC microbiology
Issue Date:
31-Mar-2017
URI:
http://hdl.handle.net/10033/620890
DOI:
10.1186/s12866-017-0987-5
PubMed ID:
28359254
Type:
Article
Language:
en
ISSN:
1471-2180
Appears in Collections:
publications of the research group microbial diagnosis (MIDI)

Full metadata record

DC FieldValue Language
dc.contributor.authorPereira, Rui P Aen
dc.contributor.authorPeplies, Jörgen
dc.contributor.authorBrettar, Ingriden
dc.contributor.authorHöfle, Manfred Gen
dc.date.accessioned2017-04-07T08:45:33Z-
dc.date.available2017-04-07T08:45:33Z-
dc.date.issued2017-03-31-
dc.identifier.citationDevelopment of a genus-specific next generation sequencing approach for sensitive and quantitative determination of the Legionella microbiome in freshwater systems. 2017, 17 (1):79 BMC Microbiol.en
dc.identifier.issn1471-2180-
dc.identifier.pmid28359254-
dc.identifier.doi10.1186/s12866-017-0987-5-
dc.identifier.urihttp://hdl.handle.net/10033/620890-
dc.description.abstractNext Generation Sequencing (NGS) has revolutionized the analysis of natural and man-made microbial communities by using universal primers for bacteria in a PCR based approach targeting the 16S rRNA gene. In our study we narrowed primer specificity to a single, monophyletic genus because for many questions in microbiology only a specific part of the whole microbiome is of interest. We have chosen the genus Legionella, comprising more than 20 pathogenic species, due to its high relevance for water-based respiratory infections.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleDevelopment of a genus-specific next generation sequencing approach for sensitive and quantitative determination of the Legionella microbiome in freshwater systems.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.en
dc.identifier.journalBMC microbiologyen

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